【摘要】：背景:糖皮質(zhì)激素的應(yīng)用已成為中國(guó)股骨頭壞死的主要因素,建立一個(gè)可信的、有效的、低死亡率的激素性股骨頭壞死模型,可為更好的探索股骨頭壞死的治療創(chuàng)造條件。目的:通過聯(lián)合靜脈注射不同劑量的馬血清和不同劑量的糖皮質(zhì)激素以建立激素誘導(dǎo)型早期股骨頭壞死兔模型。(10方法:將30只健康雄性新西蘭白兔隨機(jī)分為6組,其中4組分別耳緣靜脈注射低、高劑量馬血清,20 mg/kg)并聯(lián)合肌肉注射低、高劑量地塞米松(5,10 mg/kg)進(jìn)行造模,地塞米松組僅用高劑量地塞米松(10 mg/kg)造模,對(duì)照組靜脈注射不同劑量生理鹽水(20,2 mL/kg)。結(jié)果與討論:(1)MRI骨壞死異常信號(hào):高劑量地塞米松聯(lián)合高劑量馬血清組白兔的股骨頭在干預(yù)后第2周于MRI上首先出現(xiàn)骨壞死異常信號(hào),而除對(duì)照組外的其他4組則在干預(yù)后第4周出現(xiàn)骨壞死異常信號(hào)。干預(yù)后第6周,高劑量地塞米松聯(lián)合高劑量馬血清組中80%的兔表現(xiàn)出骨壞死信號(hào),顯著高于低劑量地塞米松聯(lián)合高劑量馬血清組(50%)、高劑量地塞米松聯(lián)合低劑量馬血清組(40%)、低劑量地塞米松聯(lián)合高劑量馬血清組(25%)、地塞米松組(20%)(P0.05);(2)血脂水平:地塞米松聯(lián)合馬血清組血清三酰甘油和總膽固醇水平在進(jìn)行第1次地塞米松注射后的第3,7,14,30天明顯高于對(duì)照組(P0.05);(3)空骨陷窩程度:與對(duì)照組相比,高劑量地塞米松聯(lián)合高劑量馬血清組空骨陷窩程度有輕度增加(P0.05);(4)結(jié)果說明,高劑量地塞米松聯(lián)合高劑量馬血清組能夠成功建立早期激素性股骨頭壞死模型,且更安全有效。
[Abstract]:Background: the application of glucocorticoid has become the main factor of osteonecrosis of femoral head in China. To establish a credible, effective and low mortality model of steroid-induced osteonecrosis of femoral head can create conditions for better exploration of treatment of osteonecrosis of femoral head. Objective: to establish a steroid-induced early necrotic rabbit model of femoral head by intravenous injection of different doses of horse serum and glucocorticoid. (10 methods: 30 healthy male New Zealand white rabbits were randomly divided into 6 groups. In the 4 groups, low, high dose horse serum (20 mg/kg) was injected intravenously into the ear margin and combined with intramuscular injection of low and high dose dexamethasone (5, 10 mg/kg). The dexamethasone group was made only by high dose dexamethasone (10 mg/kg). The control group was intravenously injected with different doses of saline (20, 2 mL/kg). Results and discussion: (1) abnormal signal of MRI osteonecrosis: the femoral head of rabbits in high-dose dexamethasone combined with high-dose horse serum group first appeared abnormal signal of osteonecrosis on MRI at the 2nd week after intervention. Except the control group, the other 4 groups showed abnormal osteonecrosis signal at the 4th week after intervention. At the 6th week after intervention, 80% of rabbits in the high-dose dexamethasone combined with high-dose horse serum group showed osteonecrosis signal, which was significantly higher than that in the low-dose dexamethasone combined with high-dose horse serum group (50%). High-dose dexamethasone combined with low-dose horse serum group (40%), low-dose dexamethasone combined with high-dose horse serum group (25%), dexamethasone group (20%) (P0.05); (2) Blood lipid level: the levels of serum triglyceride and total cholesterol in dexamethasone combined with horse serum group were significantly higher than those in control group at 3,7,14,30 days after the first injection of dexamethasone (P0.05). (3) the degree of empty bone lacuna: compared with the control group, the degree of empty bone lacuna in the high-dose dexamethasone combined with high-dose horse serum group increased slightly (P0.05); (4) the results showed that the early steroid-induced osteonecrosis of femoral head could be successfully established in the high-dose dexamethasone combined with high-dose horse serum group, and it was more safe and effective.
【作者單位】： 南京中醫(yī)藥大學(xué);江蘇省中醫(yī)院;淮安市中醫(yī)院;宣城市中醫(yī)院;
【基金】：江蘇省2011年度“六大人才高峰”D類資金(2011-WS-102)~~
【分類號(hào)】：R681.8;R-332

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