【摘要】：目的探討TRPV3通道在干酵母致發(fā)熱大鼠下丘腦組織中的表達變化及其對體溫的影響。方法采用20%干酵母混懸液背部皮下注射復制發(fā)熱大鼠模型(模型組),對照組大鼠皮下注射相應體積的生理鹽水。觀察大鼠的體溫、下丘腦組織Ca~(2+)濃度和環(huán)磷酸腺苷的變化;通過RT-PCR和Western blot法檢測TRPV3通道在下丘腦中的表達變化趨勢,分析其與體溫之間的相關性。結果模型組大鼠的體溫在注射干酵母混懸液后明顯上升(P0.01)。模型組大鼠下丘腦組織環(huán)磷酸腺苷與對照組比較顯著增加(P0.01),Ca~(2+)濃度差異無統(tǒng)計學意義(P0.05)。模型組大鼠下丘腦組織TRPV3表達趨勢與對照組比較顯著增多(P0.05);TRPV3蛋白表達量變化與體溫變化值呈正相關(P0.05)。結論干酵母導致大鼠發(fā)熱過程中,下丘腦組織TRPV3通道被激活,參與體溫調(diào)節(jié)。
[Abstract]:Objective to investigate the expression of TRPV3 channel in hypothalamus of rats with fever induced by dry yeast and its effect on body temperature. Methods Fever rats were subcutaneously injected with 20% dry yeast suspension (model group), and rats in control group were subcutaneously injected with normal saline. The changes of temperature, the concentration of Ca~ (2) and cyclic adenosine monophosphate in hypothalamus of rats were observed, and the expression trend of TRPV3 channel in hypothalamus was detected by RT-PCR and Western blot methods, and the correlation between TRPV3 channel and body temperature was analyzed. Results the body temperature of the model group increased significantly after injection of dry yeast suspension (P0.01). The concentration of cyclic adenosine monophosphate in the model group was significantly higher than that in the control group (P0.01). There was no significant difference in the concentration of Ca~ (2) between the model group and the control group (P0.05). The expression trend of TRPV3 in hypothalamus of the model group was significantly higher than that of the control group (P0.05), and the expression of TRPV3 protein was positively correlated with the change of body temperature (P0.05). Conclusion in the process of fever induced by dry yeast, the TRPV3 channel in hypothalamus is activated and participates in the regulation of temperature.
【作者單位】： 江西中醫(yī)藥大學科教學院;江西中醫(yī)藥大學實驗動物科技中心;江西中醫(yī)藥大學藥學院;
【基金】：國家自然科學基金資助項目(編號:81603402) 江西省衛(wèi)生廳中醫(yī)藥科研基金課題(編號:2013A061)
【分類號】：R33
，

本文編號：2432864