【摘要】：目的:建立Ph~+急性淋巴細(xì)胞白血病(ALL)小鼠模型,為Ph~+ALL研究提供工具。方法:用M ig190逆轉(zhuǎn)錄病毒轉(zhuǎn)染BABL/c小鼠骨髓細(xì)胞產(chǎn)生CML樣小鼠,分選CML樣小鼠BCR-ABL~+B細(xì)胞,輸注同系小鼠建立Ph~+ALL模型;應(yīng)用流式細(xì)胞術(shù)鑒定免疫表型,RT-PCR和Western blot鑒定BCR-ABL轉(zhuǎn)錄和蛋白表達(dá)。結(jié)果:M ig190逆轉(zhuǎn)錄病毒轉(zhuǎn)染后小鼠迅速發(fā)生CM L樣病變,輸注來(lái)自CM L樣小鼠的BCR-ABL~+B細(xì)胞后受鼠均發(fā)生急性淋巴細(xì)胞白血病,免疫分型顯示白血病細(xì)胞CD19~+;RT-PCR和Western Blot均檢測(cè)到BCR-ABL,符合Ph~+ALL的免疫表型和分子生物學(xué)特征。結(jié)論:成功開(kāi)發(fā)了一種新型Ph~+ALL小鼠模型的建立方法,能夠?yàn)檠芯縋h~+ALL提供一有力工具。
[Abstract]:Objective: to establish Ph~ acute lymphoblastic leukemia (Ph~) (ALL) mouse model and to provide a tool for the study of Ph~ ALL. Methods: M ig190 retrovirus was transfected into the bone marrow cells of BABL/c mice to produce CML like mice. BCR-ABL~ B cells of CML like mice were selected and the Ph~ ALL model was established in the same strain mice. Flow cytometry was used to identify the immunophenotype, and RT-PCR and Western blot were used to identify the transcription and protein expression of BCR-ABL. Results: M ig190 retrovirus-transfected mice developed CM L-like lesions rapidly, and acute lymphoblastic leukemia (ALL) occurred in all recipient mice after transfection of BCR-ABL~ B cells from CM L-like mice. Both RT-PCR and Western Blot detected BCR-ABL, in accordance with the immunophenotype and molecular biological characteristics of Ph~ ALL. Conclusion: a new Ph~ ALL mouse model was successfully developed, which can provide a powerful tool for the study of Ph~ ALL.
【作者單位】： 徐州醫(yī)科大學(xué)血液病研究所;徐州醫(yī)科大學(xué)附屬醫(yī)院血液科;
【基金】：國(guó)家自然科學(xué)基金(81471580、81272206、81300399) 江蘇省普通高校研究生科研創(chuàng)新計(jì)劃(SJEE14-0199)
【分類號(hào)】：R733.71;R-332

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