【摘要】：目的研究銀杏葉黃酮對(duì)急性腦梗死模型小鼠血清低氧誘導(dǎo)因子-1α(HIF-1α)、腫瘤壞死因子-α(TNF-α)及半胱天冬酶-3(caspase-3)影響。方法選取昆明雌性小鼠24只,隨機(jī)選取8只為假手術(shù)組,剩下小鼠進(jìn)行急性腦梗死模型的構(gòu)建。將模型小鼠按照體重隨機(jī)分成模型組和實(shí)驗(yàn)組,每組8只,實(shí)驗(yàn)組每天腹腔注射20 mg·kg~(-1)銀杏葉黃酮,假手術(shù)組和模型組同樣給藥方式給予0.9%NaCl,連續(xù)7 d。處死小鼠,用酶聯(lián)免疫吸附法檢測(cè)各組急性腦梗死模型小鼠血清中HIF-1α、TNF-α及Caspase-3的表達(dá)水平。結(jié)果假手術(shù)組、模型組、實(shí)驗(yàn)組小鼠血清中的TNF-α分別為(5.16±1.04),(33.02±20.34),(22.64±10.17)ng·mL~(-1);這3組小鼠血清中的HIF-1α分別為(508.94±123.18),(735.19±324.05),(626.24±212.76)ng·mL~(-1);這3組小鼠血清中的Caspase-3分別為(6.54±0.24),(12.23±3.45),(10.24±1.58)ng·mL~(-1);模型組小鼠血清中TNF-α、HIF-1α、Caspase-3表達(dá)明顯高于假手術(shù)組,差異均有統(tǒng)計(jì)學(xué)意義(均P0.05);實(shí)驗(yàn)組小鼠血清中TNF-α、HIF-1α、Caspase-3表達(dá)明顯低于模型組,差異均有統(tǒng)計(jì)學(xué)意義(均P0.05)。結(jié)論銀杏葉黃酮能夠明顯下調(diào)急性腦梗死小鼠血清中TNF-α、Caspase-3及HIF-1α水平。
[Abstract]:Objective to study the effects of ginkgo biloba flavonoids on serum hypoxia inducible factor-1 偽 (HIF-1 偽), tumor necrosis factor- 偽 (TNF- 偽) and cysteinase 3 (caspase-3) in mice with acute cerebral infarction. Methods Twenty-four Kunming female mice were selected and 8 mice were randomly selected as sham-operated group. The remaining mice were used to construct acute cerebral infarction model. The model mice were randomly divided into model group and experimental group according to their body weight. The experimental group received 20 mg kg~ (-1) ginkgo biloba flavone intraperitoneally every day. The sham operation group and the model group were given 0.9 NaCl3 for 7 days. The expression levels of HIF-1 偽, TNF- 偽 and Caspase-3 in serum of the mice with acute cerebral infarction were detected by enzyme-linked immunosorbent assay (Elisa). Results the levels of TNF- 偽 in serum of sham operation group, model group and experimental group were (5.16 鹵1.04), (33.02 鹵20.34), (22.64 鹵10.17) ng mL~ (-1); The HIF-1 偽 in serum of the three groups was (508.94 鹵123.18), () 735.19 鹵324.05), (626.24 鹵212.76) ng mL~ (-1); The Caspase-3 in serum of the three groups was (6.54 鹵0.24), (12.23 鹵3.45), (10.24 鹵1.58) ng mL~ (-1); The expression of TNF- 偽, HIF-1 偽 and Caspase-3 in serum of model group was significantly higher than that of sham operation group (P0.05). The expression of TNF- 偽, HIF-1 偽 and Caspase-3 in serum of experimental group was significantly lower than that of model group (P0.05). Conclusion Ginkgo biloba flavonoids can significantly decrease the levels of TNF- 偽, Caspase-3 and HIF-1 偽 in serum of mice with acute cerebral infarction.
【作者單位】： 南方醫(yī)科大學(xué)附屬南方醫(yī)院神經(jīng)內(nèi)科;廣州軍區(qū)廣州總醫(yī)院神經(jīng)內(nèi)科;
【基金】：廣東省科技計(jì)劃基金資助項(xiàng)目(2013B012800164)
【分類號(hào)】：R285.5;R-332

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 李旭光,樸金建;銀杏葉黃酮擴(kuò)血管機(jī)理的探討[J];佳木斯醫(yī)學(xué)院學(xué)報(bào);1998年02期

2 徐劍,袁如彩,秦延年;銀杏葉黃酮口服液的質(zhì)量控制方法[J];徐州醫(yī)學(xué)院學(xué)報(bào);1998年03期

3 周君富;銀杏葉黃酮的抗脂質(zhì)過氧化作用[J];中藥藥理與臨床;1994年02期

4 張林濤;張鋒;王志祥;巫先坤;蔡錦源;;銀杏葉黃酮提取工藝的優(yōu)化[J];中國藥物警戒;2011年03期

5 耿秀芳,張言鎮(zhèn),李桂芝,趙云貴,康白;銀杏葉黃酮擴(kuò)血管機(jī)制的實(shí)驗(yàn)研究[J];中國中醫(yī)藥科技;1999年04期

6 吳東方,羅順德,馮小東,冷臘英,徐玲君;銀杏葉黃酮對(duì)肝臟MDA生成的影響[J];中國中藥雜志;1997年01期

7 李萬忠;張曉平;崔清華;丁嘉信;田景振;;銀杏葉黃酮在D201陰離子樹脂上的靜態(tài)吸附動(dòng)力學(xué)研究[J];中國藥房;2013年11期

8 高錦明，張鞍靈;銀杏葉黃酮提取及含量測(cè)定[J];陜西林業(yè)科技;1996年04期

9 陳季武,胡天喜,朱振勤;銀杏葉黃酮抗氧化作用的研究[J];華東師范大學(xué)學(xué)報(bào)(自然科學(xué)版);1998年03期

10 吳鵬;郭峰;陳龍;趙巖;李平亞;;銀杏葉黃酮的酶法提取及生物轉(zhuǎn)化研究[J];特產(chǎn)研究;2007年04期

相關(guān)碩士學(xué)位論文 前2條

1 劉金波;銀杏葉黃酮檢測(cè)方法及提取工藝優(yōu)化研究[D];沈陽農(nóng)業(yè)大學(xué);2016年

2 薛志彬;銀杏葉有效部位的提取工藝及注射液成型工藝的研究[D];遼寧醫(yī)學(xué)院;2011年

，

本文編號(hào)：2417247