【摘要】：目的比較淡色庫蚊抗吡丙醚種群和敏感種群羧酸酯酶生化特征的差異,初步探討其生化抗性機制。方法參照van Aspern(1962)的方法測定羧酸酯酶活性。結(jié)果淡色庫蚊抗性品系和敏感品系羧酸酯酶水解活性隨底物濃度(α-乙酸萘酯或β-乙酸萘酯)的增加而升高;在一定濃度范圍內(nèi),抗性品系對這兩種底物的水解活性均高于敏感品系。β-乙酸萘酯為底物時,抗性品系羧酸酯酶的米氏常數(shù)(K_m)和最大反應(yīng)速度(V_(max))分別為27.20×10~(-5)mol/L和115.00×10~(-5)A/(mg·pro·min),敏感品系K_m和V_(max)分別為104.00×10~(-5)mol/L和207.00×10~(-5)A/(mg·pro·min),兩者差異有統(tǒng)計學(xué)意義(t_b=2.740,t_c=3.160,P0.05)。α-乙酸萘酯為底物時,抗性品系羧酸酯酶K_m為131.00×10~(-5)mol/L,與敏感品系K_m(75.20×10~(-5)mol/L)比較差異無統(tǒng)計學(xué)意義,而V_(max)則差異有統(tǒng)計學(xué)意義。膽堿酯酶抑制劑測定結(jié)果表明,抗性品系羧酸酯酶對敵敵畏和殘殺威的敏感性高于敏感品系。結(jié)論羧酸酯酶可能與淡色庫蚊對吡丙醚的抗性有關(guān)。
[Abstract]:Objective to compare the biochemical characteristics of carboxylesterase of Culex pipiens pallens resistant to pyridine ether and sensitive populations and to explore the biochemical resistance mechanism of Culex pipiens pallens. Methods carboxylesterase activity was determined by van Aspern (1962. Results carboxylesterase hydrolysis activity of resistant and sensitive strains of Culex pipiens pallens increased with the increase of substrate concentration (偽 -naphthalene acetate or 尾 -naphthalene acetate). In a certain concentration range, the hydrolytic activity of the resistant strains to these two substrates was higher than that of the sensitive strains. When 尾 -naphthalene acetate was used as substrate, The Michaelis constant (km) and maximum reaction rate (V _ (max) of carboxylesterase of resistant strains are 27.20 脳 10 ~ (-5) mol/L and 115.00 脳 10 ~ (-5) A / (mg pro min), respectively. For sensitive strains km and V _ (max) were 104.00 脳 10 ~ (-5) mol/L and 207.00 脳 10 ~ (-5) A / (mg pro min), respectively. When 偽 -naphthalene acetate was used as substrate, there was no significant difference between resistant strain Kapm (131.00 脳 10 ~ (-5) mol/L,) and sensitive strain K _ m (75.20 脳 10 ~ (-5) mol/L). The difference of V _ (max) was statistically significant. The results of cholinesterase inhibitor assay showed that the sensitivity of resistant line carboxylesterase to dichlorvos and propoxur was higher than that of sensitive strain. Conclusion carboxylesterase may be related to the resistance of Culex pipiens pallens to pyridine ether.
【作者單位】： 上海市疾病預(yù)防控制中心病媒生物防治科;
【基金】：上海市自然科學(xué)基金(12ZR1426600)~~
【分類號】：R384.1

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