【摘要】：目的:研究枳實及其主要活性成分促進脾虛模型大鼠胃腸運動的機制。方法:將170只大鼠隨機分為空白組(10只)和造模組(160只),造模組大鼠以苦寒瀉下+饑飽失常法復制脾虛模型。造模后,將大鼠隨機分為模型組,柚皮苷低、中、高劑量組(3.267、6.535、13.070 mg/mL),新橙皮苷低、中、高劑量組(3.865、7.730、15.460 mg/mL),辛弗林低、中、高劑量組(0.252、0.504、1.008 mg/mL),3個單體成分(即柚-新-辛)低、中、高劑量配伍組和枳實水煎液低、中、高劑量組(0.104、0.208、0.416 g/mL,以生藥計),每天ig給藥1次,10 mL/kg,連續(xù)7 d。末次給藥后,檢測大鼠血清胃泌素(GAS)和血漿乙酰膽堿(ACh)、胃動素(MTL)、P物質(SP)、血管活性腸肽(VIP)水平。結果:與空白組比較,模型組大鼠血清GAS水平和血漿ACh、MTL、SP水平降低(P0.01),血漿VIP水平升高(P0.05)。與模型組比較,除柚皮苷高劑量組和辛弗林各劑量組大鼠血清GAS水平外,其余各給藥組均明顯升高(P0.05或P0.01);新橙皮苷高劑量組、辛弗林高劑量組和枳實水煎液低劑量組大鼠血漿ACh水平顯著升高(P0.01);新橙皮苷中、高劑量組,辛弗林高劑量組,低、中劑量配伍組和枳實水煎液中、高劑量組大鼠血漿MTL水平顯著升高(P0.05);柚皮苷低、中劑量組和新橙皮苷各劑量組大鼠血漿SP水平顯著升高(P0.05或P0.01);柚皮苷低劑量組,辛弗林高劑量組和枳實水煎液低、中劑量組大鼠血漿VIP水平顯著降低(P0.05)。結論:枳實可能通過促進GAS、ACh、MTL的分泌和抑制VIP的分泌來促進脾虛模型大鼠的胃腸運動;其與3個單體成分對胃腸激素的調節(jié)作用有差異。
[Abstract]:Aim: to study the mechanism of promoting gastrointestinal motility of rats with spleen deficiency by Aurantii aurantii and its main active components. Methods: 170 rats were randomly divided into blank group (n = 10) and model group (n = 160). After modeling, rats were randomly divided into three groups: model group with low, medium and high doses of naringin (3.2676.535U 13.070 mg/mL), low, medium and high dose groups (3.8657.730c15.460 mg/mL), low and medium dose of synephrine (3.2676.535U 13.070 mg/mL), high dose group (3.8657.730c15.460 mg/mL), low dose group (3.2676.535U 13.070 mg/mL). In the high dose group (0.252 ~ 0.504 ~ 1.008 mg/mL), the three monomer components (namely grapefruit-Xinxin) were low, the middle, high dose compatibility groups and Fructus Aurantii decoction were low, and the middle and high dose groups (0.104 ~ 0.208 ~ 0.208 ~ (0.416) g / mL, in terms of crude drugs). Ig was given once a day for 10 mL/kg, for 7 days. The levels of serum gastrin (GAS) and plasma acetylcholine (ACh), motilin (MTL), P substance (SP), vasoactive intestinal peptide (VIP) were measured after the last administration. Results: compared with the blank group, the serum GAS level and plasma ACh,MTL,SP level in the model group decreased (P0.01), and the plasma VIP level increased (P0.05). Compared with the model group, the serum GAS level of the rats in the high dose of naringin group and the Simverin group was significantly increased (P0.05 or P0.01), and the level of serum GAS in the other groups was significantly higher than that in the model group (P0.05 or P0.01). The plasma ACh levels in high dose group, high dose group and low dose group of Fructus aurantii were significantly increased (P0.01). The levels of plasma MTL in high dose group, low, middle dose compatibility group and Fructus Aurantii decoction were significantly increased (P0.05). Naringin was low and plasma SP level was significantly increased in middle dose group and nehesperidin group (P0.05 or P0.01). Naringin low dose group, Simferin high dose group and Fructus Aurantii decoction were low, the plasma VIP level was significantly decreased in middle dose group (P0.05). Conclusion: Fructus Aurantii can promote the gastrointestinal motility of spleen deficiency rats by promoting the secretion of GAS,ACh,MTL and inhibiting the secretion of VIP.
【作者單位】： 江西中醫(yī)藥大學現(xiàn)代中藥制劑教育部重點實驗室;
【基金】：國家自然科學基金資助項目(No.81460575,81260612)
【分類號】：R285.5;R-332

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