【摘要】：目的觀察人體皮膚類(lèi)似物模型形態(tài)結(jié)構(gòu),對(duì)其屏障功能進(jìn)行初步評(píng)價(jià)。方法通過(guò)膠原蛋白包被成纖維細(xì)胞,制備真皮層,真皮層表面接種角質(zhì)細(xì)胞,浸沒(méi)培養(yǎng)1周后,氣-液界面培養(yǎng)以促進(jìn)角質(zhì)細(xì)胞的分化形成角質(zhì)層,3周后收獲皮膚模型;通過(guò)肉眼觀察、電鏡觀察皮膚類(lèi)似物的宏觀結(jié)構(gòu)和超微結(jié)構(gòu);以吲哚美辛作為模型藥物,進(jìn)行體外透皮實(shí)驗(yàn)考察細(xì)胞共培養(yǎng)皮膚類(lèi)似物模型的屏障功能。結(jié)果皮膚類(lèi)似物形成與人類(lèi)皮膚相似的真皮層和表皮層,表皮層表面形成完整的角質(zhì)層,具有與人類(lèi)皮膚類(lèi)似的超微結(jié)構(gòu);24 h內(nèi)滲透性實(shí)驗(yàn)結(jié)果顯示皮膚類(lèi)似物屏障功能略低于大鼠腹部皮膚,但22~24 h,皮膚類(lèi)似物對(duì)模型藥的透過(guò)速率遠(yuǎn)小于大鼠腹部皮膚(約為其28%),且2種皮膚模型對(duì)吲哚美辛經(jīng)皮滲透的穩(wěn)態(tài)流速?zèng)]有顯著性差異(P0.05)。結(jié)論皮膚類(lèi)似物有與人體皮膚相類(lèi)似的形態(tài)結(jié)構(gòu)特征,與大鼠腹部皮膚相比對(duì)吲哚美辛有相似的屏障作用,有望替代動(dòng)物皮膚成為經(jīng)皮給藥系統(tǒng)評(píng)價(jià)的新型皮膚模型。
[Abstract]:Objective to observe the morphologic structure of human skin analogue model and evaluate its barrier function. Methods the fibroblasts were coated with collagen protein to prepare the dermis. The keratinocytes were inoculated on the surface of the dermis. After 1 week of immersion and culture, the air-liquid interface was cultured to promote the differentiation of the keratinocytes into the keratinocytes, and the skin model was harvested after 3 weeks. The macrostructure and ultrastructure of the skin analogue were observed by naked eye and electron microscope, and the barrier function of the skin analogue model co-cultured by cell culture was investigated by transdermal experiments in vitro with indomethacin as the model drug. Results the skin analogue formed the dermis and epidermis similar to the human skin, and the surface of the epidermis formed a complete cuticle, which had the ultrastructure similar to that of the human skin. The results of permeability test within 24 h showed that the barrier function of skin analogue was slightly lower than that of abdominal skin of rats, but the permeation rate of skin analogue to the model drug was much lower than that of abdominal skin (about 28%) at 22 ~ 24 h. There was no significant difference in the steady flow rate of indomethacin permeation between the two skin models (P0.05). Conclusion the skin analogue has similar morphological and structural characteristics to human skin and has similar barrier effect to indomethacin compared with the abdominal skin of rats. It is expected to replace animal skin as a new skin model for the evaluation of drug delivery system.
【作者單位】： 北京中醫(yī)藥大學(xué)中藥學(xué)院;北京中醫(yī)藥大學(xué)科研實(shí)驗(yàn)中心;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(No.81473365) 北京市自然科學(xué)基金項(xiàng)目(No.7132127) 北京中醫(yī)藥大學(xué)創(chuàng)新團(tuán)隊(duì)發(fā)展計(jì)劃項(xiàng)目(No.2011-CXTD-13);北京中醫(yī)藥大學(xué)自主選題項(xiàng)目(No.2015-JYB-XS095)
【分類(lèi)號(hào)】：R329.2

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