【摘要】：目的觀察黃芩苷對(duì)潰瘍性結(jié)腸炎模型大鼠核因子?κB(Nuclear factor?κB,NF-κB)表達(dá)及下游炎癥細(xì)胞因子的影響。方法采用TNBS法制備大鼠潰瘍性結(jié)腸炎模型,隨機(jī)分為正常組、模型組、黃芩苷(Baicalin)高中低劑量組、陽(yáng)性對(duì)照組(柳氮磺胺吡啶,SASP組)。給藥10d后,留取結(jié)腸組織標(biāo)本,觀察結(jié)腸大體形態(tài)變化,采用ELISA法觀察UC模型大鼠結(jié)腸組織中腫瘤壞死因子(TNF-α)、白介素-8(IL-8)和白介素-1(IL-1)的含量,采用Western blot法檢測(cè)NF-κB的表達(dá)水平。結(jié)果與模型組相比,黃芩苷100mg/kg組能顯著改善結(jié)腸大體形態(tài),黃芩苷(25、50、100mg/kg)干預(yù)后能劑量依賴性的抑制各炎癥因子的分泌,黃芩苷高劑量組能降低IL-1、IL-8和TNF-α表達(dá)水平,但與SASP組比較無(wú)明顯差異。黃芩苷高劑量組能降低NF-κB的水平,促進(jìn)IκBα水平(P0.05)。結(jié)論黃芩苷能夠抑制NF-κB的活化,從而發(fā)揮其在潰瘍性結(jié)腸炎中的抗炎效應(yīng)。
[Abstract]:Objective to observe the effect of baicalin on the expression of nuclear factor-魏 B (Nuclear factor? 魏 B-魏 NF- 魏 B and inflammatory cytokines in rats with ulcerative colitis. Methods the ulcerative colitis model of rats was established by TNBS method. The rats were randomly divided into normal group, model group, baicalin (Baicalin) high and low dose group, and positive control group (SASP group). After 10 days of administration, the colonic tissue samples were collected to observe the changes of colonic gross morphology, and the tumor necrosis factor (TNF- 偽) in colonic tissue of UC model rats was observed by ELISA method. The levels of interleukin-8 (IL-8) and interleukin-1 (IL-1) were detected by Western blot assay. Results compared with the model group, baicalin 100mg/kg group could significantly improve the colonic gross morphology. Baicalin (2550 mg / kg) could inhibit the secretion of inflammatory factors in a dose-dependent manner, while baicalin high dose group could reduce IL-1,. The expression levels of IL-8 and TNF- 偽 were not significantly different from those of SASP group. Baicalin high dose group could decrease the level of NF- 魏 B and promote the level of I 魏 B 偽 (P0.05). Conclusion baicalin can inhibit the activation of NF- 魏 B and exert its anti-inflammatory effect in ulcerative colitis.
【作者單位】： 南京中醫(yī)藥大學(xué)附屬醫(yī)院;南京中醫(yī)藥大學(xué)第一臨床醫(yī)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金(81403344) 國(guó)家中醫(yī)藥行業(yè)科研專項(xiàng)資助(201407001) 江蘇省臨床醫(yī)學(xué)科技專項(xiàng)項(xiàng)目資助(BL2014100) 國(guó)家中醫(yī)臨床研究基地(脾胃)資助 江蘇省高校優(yōu)勢(shì)學(xué)科項(xiàng)目資助
【分類號(hào)】：R285.5;R-332

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 趙寶玲;任現(xiàn)志;;膜增生性腎小球腎炎與NF-κB的關(guān)系及中醫(yī)藥研究進(jìn)展[J];中華中醫(yī)藥雜志;2013年02期

2 于俊生;王強(qiáng);于惠青;;升降散對(duì)系膜增生性腎小球腎炎大鼠腎組織NF-κB表達(dá)的影響[J];中國(guó)實(shí)驗(yàn)方劑學(xué)雜志;2011年10期

3 鄭建明;陳曉春;林敏;張靜;林智穎;鄭關(guān)毅;李康增;;赤芍801通過(guò)抑制NF-κB抗腦缺血-再灌注損傷的機(jī)制[J];藥學(xué)學(xué)報(bào);2011年02期

4 栗明;栗德林;于陽(yáng);;芪玄益心膠囊對(duì)大鼠心肌NF-κBp50mRNA IκBαamRNA的影響[J];中華中醫(yī)藥學(xué)刊;2008年09期

5 張娜;李琳;高向暉;蔡慧珍;陶秀娟;趙q，

本文編號(hào)：2375878