【摘要】：目的探討體內(nèi)心肌微環(huán)境及體外5-氮雜胞苷(5-aza)誘導(dǎo)脂肪間充質(zhì)干細(xì)胞分化為心肌樣細(xì)胞的作用差異。方法取小鼠腹股溝的皮下脂肪組織,分離培養(yǎng)脂肪間充質(zhì)干細(xì)胞(ADMSCs),對(duì)其進(jìn)行表面標(biāo)記和成骨、成脂分化能力鑒定。選取生長(zhǎng)狀態(tài)良好的第3代ADMSCs,分為5-aza體外誘導(dǎo)組、體內(nèi)心肌移植組,體外誘導(dǎo)3周以及體內(nèi)移植1周后,采用免疫熒光技術(shù)檢測(cè)特異性心肌肌鈣蛋白T(cT nT)的表達(dá)。結(jié)果兩種誘導(dǎo)方法ADMSCs均表達(dá)cT nT,5-aza誘導(dǎo)組3周表達(dá)率(33.33±3.79)%,移植組1周表達(dá)率(42.93±4.04)%,移植組1周較5-aza誘導(dǎo)組3周具有更高的分化效率(P0.05)。結(jié)論體外5-aza化學(xué)誘導(dǎo)和體內(nèi)心肌微環(huán)境均可使ADMSCs分化為心肌樣細(xì)胞,但心肌微環(huán)境的誘導(dǎo)分化效率明顯高于5-aza的作用。
[Abstract]:Objective to investigate the effects of 5-azacytidine (5-aza) on differentiation of adipose mesenchymal stem cells into cardiomyocyte-like cells in vivo and in vitro. Methods the subcutaneous adipose tissue of mouse groin was isolated and cultured with (ADMSCs), for surface labeling and osteogenesis, and the ability of adipogenic differentiation was evaluated. The third generation of ADMSCs, with good growth state was divided into three groups: in vitro 5-aza induction group, in vivo myocardial transplantation group. The expression of cardiac troponin T (cT nT) was detected by immunofluorescence technique after 3 weeks of in vitro induction and 1 week after in vivo transplantation. Results the expression rate of cT nT,5-aza was (33.33 鹵3.79)% in ADMSCs group and (42.93 鹵4.04)% in transplantation group at 1 week. The differentiation efficiency of transplantation group was higher than that of 5-aza induction group at 1 week (P0.05). Conclusion both chemical induction of 5-aza in vitro and myocardial microenvironment in vivo can induce the differentiation of ADMSCs into cardiomyocyte-like cells, but the differentiation efficiency of myocardial microenvironment is significantly higher than that of 5-aza.
【作者單位】： 新鄉(xiāng)醫(yī)學(xué)院河南省醫(yī)用組織再生重點(diǎn)實(shí)驗(yàn)室;新鄉(xiāng)市中心醫(yī)院心內(nèi)科;
【基金】：National Natural Science Foundation of China(81400237) Key Project of He’nan Provincial Health Bureau(201403135) Xinxiang Medical University Graduate Student Research and Innovation Support Program(YJSCX201302Z)
【分類號(hào)】：R329.2

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