【摘要】：目的觀察瘦素對心肌缺血-再灌注損傷(MIRI)大鼠模型的心肌影響,探討瘦素在MIRI中的作用,闡明瘦素在心肌缺血-再灌注損傷的重要病理生理意義,并指導心肌缺血-再灌注損傷疾病的臨床預防與治療。 方法:24只雄性SD大鼠,按隨機數(shù)字表法分為4組(n=6):①假手術(shù)(sham)組、②標準MIRI組、③瘦素預處理(Leptin+MIRI)組和④瘦素處理(MIRI+Leptin)組。③組大鼠于MIRI術(shù)前30 min予腹腔注射重組瘦素(美國RD Systems公司),劑量為50μg/kg。④組大鼠于MIRI手術(shù)后30 min予腹腔注射重組瘦素,劑量為50μg/kg。以上4組大鼠的標本均于MIRI術(shù)后120 min采集。觀察心肌梗死面積、心肌瘦素水平以及血清瘦素、磷酸肌酸激酶(CK)、乳酸脫氫酶(LDH)、天冬氨酸轉(zhuǎn)氨酶(AST)、肌鈣蛋白(TnT)水平。 結(jié)果瘦素處理組心肌梗死面積〔(39.43±2.06)%〕、心肌瘦素水平〔(23.1±2.95)μg/g〕、血清瘦素水平〔(38.37±1.98)μg/g〕顯著高于MIRI組心肌梗死面積〔(33.20±2.12)%〕、心肌瘦素水平〔(15.50±3.14)μg/g〕血清瘦素水平〔(29.49±2.01μg/g〕;瘦素預處理組心肌梗死面積〔(9.20±1.85)%〕、心肌瘦素水平〔(8.26±3.29)μg/g〕、血清瘦素水平〔(10.31±1.88)μg/g〕顯著低于MIRI組心肌梗死面積〔(33.20±2.12)%〕、心肌瘦素水平〔(15.50±3.14)μg/g〕、血清瘦素水平〔(29.49±2.01)μg/g〕;瘦素處理組血清LDH〔(1067.4±123.2)U/L〕、血清AST〔(402.9±55.0)U/L〕、血清TnT濃度〔(0.98±0.13)μg/g〕顯著高于MIRI組LDH〔(884.5±104.3)U/L〕、血清AST〔(327.1±44.9)U/L〕、血清TnT濃度〔(0.72±0.10)μg/g〕;瘦素預處理組血清CK〔(3231.7±640.1)U/L〕、LDH〔(610.4±86.0)U/L〕、血清AST〔(202.3±29.1)U/L〕、血清TnT濃度〔(0.51±0.09)μg/g〕顯著低于MIRI組CK〔(5310.3±849.4)U/L〕、LDH〔(884.5±104.3)U/L〕、血清AST〔(327.1±44.9)U/L〕、血清TnT濃度〔(0.72±0.10)μg/g〕。 結(jié)論瘦素處理和高水平的瘦素加重了MIRI,瘦素預處理可通過減少心肌和血清的瘦素水平以減輕MIRI。
[Abstract]:Objective to observe the effects of leptin on myocardial ischemia reperfusion injury in (MIRI) rats, to explore the role of leptin in MIRI, and to elucidate the important pathophysiological significance of leptin in myocardial ischemia-reperfusion injury. To guide the clinical prevention and treatment of myocardial ischemia-reperfusion injury. Methods: Twenty-four male SD rats were randomly divided into 4 groups (n = 6): 1 sham operation (sham) group and 2 standard MIRI group. 3 leptin pretreated (Leptin MIRI) group and 4 leptin treated (MIRI Leptin) group. Three groups of rats were injected with recombinant leptin 30 min before MIRI (RD Systems Company, USA). The rats in the 50 渭 g/kg.4 group were intraperitoneally injected with recombinant leptin 30 min after MIRI operation, and the dose was 50 渭 g / kg. The specimens of the above four groups were collected at 120 min after MIRI. Myocardial infarction size, myocardial leptin level and serum leptin, (CK), lactate dehydrogenase (LDH), aspartate transaminase (AST),) troponin (TnT) were measured. Results the myocardial infarction size and myocardial leptin level in leptin treated group were (39.43 鹵2.06)% and (23.1 鹵2.95) 渭 g / g, respectively. Serum leptin level (38.37 鹵1.98 渭 g / g) was significantly higher than that in MIRI group (33.20 鹵2.12%), myocardial leptin level (15.50 鹵3.14) 渭 g / g, serum leptin level (29.49 鹵2.01 渭 g / g); Myocardial infarction size and myocardial leptin level in leptin preconditioning group were (9.20 鹵1.85)% and (8.26 鹵3.29) 渭 g / g, respectively. Serum leptin level (10.31 鹵1.88 渭 g / g) was significantly lower than that in MIRI group (33.20 鹵2.12%), and myocardial leptin level was (15.50 鹵3.14) 渭 g / g. Serum leptin level (29.49 鹵2.01) 渭 g / g; Serum LDH (1067.4 鹵123.2 U / L, serum AST (402.9 鹵55.0 UP / L, serum TnT concentration (0.98 鹵0.13) 渭 g / g in leptin treated group were significantly higher than LDH (884.5 鹵104.3 UP / L in MIRI group. Serum AST (327.1 鹵44.9 渭 g / L), serum TnT concentration (0.72 鹵0.10) 渭 g / g; Leptin pretreatment group: serum CK (3231.7 鹵640.1) L / L), LDH (610.4 鹵86.0 U / L), serum AST (202.3 鹵29.1) U / L), Serum TnT concentration (0.51 鹵0.09) 渭 g / g) was significantly lower than that in MIRI group (CK (5310.3 鹵849.4) UL), LDH (884.5 鹵104.3 UP / L, serum AST (327.1 鹵44.9 UP / L). Serum TnT concentration was (0.72 鹵0.10) 渭 g / g. Conclusion leptin treatment and high levels of leptin increase MIRI, leptin preconditioning, which can reduce MIRI. by reducing serum and myocardial leptin levels.
【學位授予單位】：桂林醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R363

【參考文獻】

相關(guān)期刊論文 前7條

1 羅艷利,劉翠平,趙彩彥,李躍林;瘦素與心血管疾病[J];國外醫(yī)學(內(nèi)科學分冊);2003年08期

2 徐彤彤;戴玲;;瘦素與急性心肌梗死溶栓后ST段變化的關(guān)系探討[J];廣西醫(yī)科大學學報;2007年03期

3 徐彤彤;陽躍忠;;急性心肌梗死患者血清瘦素水平的變化及其臨床意義[J];臨床內(nèi)科雜志;2007年02期

4 樊榮;臧益民;;脂肪組織的功與過評述[J];心臟雜志;2008年03期

5 徐彤彤;陽躍忠;;大鼠急性心梗中血漿瘦素及血流動力學參數(shù)變化的探討[J];中國臨床藥理學與治療學;2007年02期

6 劉世平;徐彤彤;;瘦素與心肌缺血/再灌注損傷關(guān)系的研究進展[J];醫(yī)學綜述;2009年04期

7 顏光濤,薛輝,林季,郝秀華,張凱,王錄煥;急性心肌梗死患者血清Leptin增高與其他相關(guān)因素的分析[J];中國危重病急救醫(yī)學;2005年09期

，

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