【摘要】：目的:探究高表達(dá)和干擾PSMA7對(duì)A549細(xì)胞周期以及RB途徑中Cyclin D1、CDK4、P16、Rb蛋白水平的影響。方法:將pcDNA3.1-PSMA7高表達(dá)和pGPU6/Hygro-PSMA7-265干擾載體分別瞬時(shí)轉(zhuǎn)染A549細(xì)胞,然后用流式細(xì)胞儀檢測(cè)PSMA7對(duì)細(xì)胞周期的影響,再通過(guò)Western blot實(shí)驗(yàn)檢測(cè)Cyclin D1、CDK4、P16、Rb的蛋白水平。結(jié)果:和對(duì)照組相比,PSMA7高表達(dá)時(shí)周期時(shí)相分布無(wú)明顯差異,但Cyclin D1、CDK4的蛋白表達(dá)水平明顯降低,P16、Rb的表達(dá)明顯增高;和對(duì)照組相比,干擾PSMA7后細(xì)胞的G0/G1、G2/M期比例均降低,而S期的值增高,均具有差異,而Cyclin D1、CDK4的蛋白表達(dá)水平明顯增加,P16、Rb的表達(dá)明顯降低,以上結(jié)果與對(duì)照組相比差異均有統(tǒng)計(jì)學(xué)意義。結(jié)論:PSMA7在A549肺腺癌細(xì)胞中能夠影響RB途徑中Cyclin D1、CDK4、P16、Rb的蛋白水平的表達(dá),干擾PSMA7使A549細(xì)胞較早進(jìn)入S期。
[Abstract]:Aim: to investigate the effects of overexpression and interference of PSMA7 on the cell cycle of A549 cells and the level of Cyclin D1CDK4P16Rb in the RB pathway. Methods: overexpression of pcDNA3.1-PSMA7 and pGPU6/Hygro-PSMA7-265 interference vector were transiently transfected into A549 cells, then the effect of PSMA7 on cell cycle was detected by flow cytometry, and the protein level of Cyclin D1CDK4P16Rb was detected by Western blot assay. Results: compared with the control group, there was no significant difference in the phase distribution at the time of high expression of PSMA7, but the expression level of CDK4 in Cyclin D1 was significantly decreased, the expression of P16 rb was increased significantly, and the ratio of G0 / G1 / G2 / M phase was decreased after PSMA7 was interfered with in the control group. The expression of CDK4 in Cyclin D1 was significantly increased and the expression of P16 rb was significantly decreased in S phase. The above results were significantly different from those in the control group. Conclusion: PSMA7 can affect the expression of Cyclin D1CDK4P16Rb in A549 lung adenocarcinoma cells, and interfere with the expression of Cyclin D1CDK4P16Rb in A549 lung adenocarcinoma cells, and interfere with PSMA7 to make A549 cells enter S phase earlier.
【作者單位】： 南方醫(yī)科大學(xué)附屬中山博愛(ài)醫(yī)院產(chǎn)前診斷中心;南方醫(yī)科大學(xué)附屬中山博愛(ài)醫(yī)院中心ICU;
【基金】：廣東省醫(yī)學(xué)科研基金項(xiàng)目(A2013875) 國(guó)家自然科學(xué)基金(81101534)資助
【分類號(hào)】：R329.2
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本文編號(hào)：2287855