【摘要】：目的探討老化骨髓間充質(zhì)干細(xì)胞(BMSCs)的自發(fā)性骨化過(guò)程及脂聯(lián)素在這一過(guò)程中的作用。方法將第2代BMSCs(分離自一小兒畸形斷肢的殘指)分為空白對(duì)照組及脂聯(lián)素低、中、高劑量組�？瞻讓�(duì)照組加入DMEM/F12培養(yǎng)基及10%胎牛血清,脂聯(lián)素低、中、高劑量組分別在此基礎(chǔ)上加入終濃度為0.5、1.0、2.0μg/m L的脂聯(lián)素,各組均傳代培養(yǎng)至第10代。收集各組第2代和第10代細(xì)胞,茜素紅S染色后計(jì)算鈣化物染色率,q PCR法檢測(cè)骨鈣素(OCN)mRNA相對(duì)表達(dá)量。結(jié)果與第2代細(xì)胞比較,各組第10代細(xì)胞鈣化物染色率、OCN mRNA相對(duì)表達(dá)量均升高(P均0.05);與空白對(duì)照組第10代細(xì)胞比較,脂聯(lián)素低、中、高劑量組鈣化物染色率、OCN mRNA相對(duì)表達(dá)量均升高,但脂聯(lián)素中、高劑量組升高更明顯(P均0.05)。結(jié)論 BMSCs在傳代過(guò)程中可發(fā)生自發(fā)性骨化,脂聯(lián)素可促進(jìn)這一過(guò)程,并呈濃度依賴性。
[Abstract]:Objective to investigate the spontaneous ossification of aging bone marrow mesenchymal stem cells (BMSCs) and the role of adiponectin in the process. Methods the second generation of BMSCs was divided into blank control group and adiponectin low, middle and high dose groups. In the blank control group, adiponectin was added to DMEM/F12 medium and 10% fetal bovine serum, and adiponectin was added to 0.51.0 渭 g / mL adiponectin at the final concentration of 0.51.0 渭 g / mL in the middle and high dose groups respectively. The adiponectin was subcultured to the 10th generation in each group. The second and tenth passage cells of each group were collected and the relative expression of osteocalcin (OCN) mRNA was detected by, q PCR method after alizarin red S staining. Results compared with the second passage cells, the relative expression of, OCN mRNA in the 10th passage cells was significantly higher than that in the second passage cells, and the adiponectin level was lower than that in the control group (P < 0.05), and the level of adiponectin was lower than that in the control group (P < 0.05). The relative expression of, OCN mRNA in high dose group was higher than that in high dose group (P 0. 05), but adiponectin in high dose group was higher than that in high dose group (P 0. 05). Conclusion spontaneous ossification may occur in the passage of BMSCs, and adiponectin can promote this process in a concentration dependent manner.
【作者單位】： 內(nèi)蒙古民族大學(xué)附屬醫(yī)院;
【基金】：內(nèi)蒙古自治區(qū)教育廳科技計(jì)劃項(xiàng)目(NJZY173)
【分類號(hào)】：R329.2

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