【摘要】：目的 本實(shí)驗(yàn)通過建立兩個(gè)不同時(shí)期的產(chǎn)前應(yīng)激大鼠動(dòng)物模型,觀察不同時(shí)期產(chǎn)前應(yīng)激對(duì)子代大鼠的行為學(xué)、細(xì)胞因子白介素18 (interleukin 18, IL-18)及細(xì)胞外信號(hào)調(diào)節(jié)激酶(Mitogn activated protein kinase kinase 1, MEK-1)的影響,從而探討產(chǎn)前應(yīng)激敏感期及性別效應(yīng)的病理生理機(jī)制,以期為與產(chǎn)前應(yīng)激相關(guān)的疾病的發(fā)病機(jī)制的研究提供依據(jù)。 方法 將SD妊娠大鼠隨機(jī)分成3組,對(duì)照組在妊娠期間不給予任何刺激；中期應(yīng)激組在從妊娠第9-14天給予束縛刺激；晚期妊娠組從妊娠第15-20天給予束縛刺激。待子代出生后第21天斷奶并將雌雄大鼠分籠飼養(yǎng)。子代成年后即出生后第75天分別進(jìn)行敞箱實(shí)驗(yàn)、新事物實(shí)驗(yàn)、蔗糖喜愛實(shí)驗(yàn)、前脈沖抑制及驚恐反射實(shí)驗(yàn)和強(qiáng)迫游泳實(shí)驗(yàn)5種行為學(xué)測(cè)試及檢測(cè)大鼠海馬的IL-18和MEK-1。 結(jié)果 一.行為學(xué)實(shí)驗(yàn)結(jié)果： 1.敞箱實(shí)驗(yàn)：結(jié)果顯示與對(duì)照組雄性子代相比孕晚期產(chǎn)前刺激雄性子代的敞箱總路程減少(p0.05),中央?yún)^(qū)域總路程減少(p0.05),中央?yún)^(qū)域停留時(shí)間減少(p0.01)。 2.新事物實(shí)驗(yàn)：結(jié)果顯示與對(duì)照組雄性子代接觸新事物的時(shí)間相比孕中期產(chǎn)期應(yīng)激組雄性子代(p0.01)及孕晚期產(chǎn)期應(yīng)激組雄性子代(p0.05)均減少。 3.糖水消耗實(shí)驗(yàn)：結(jié)果顯示與對(duì)照組雄性子代相比孕中期產(chǎn)前應(yīng)激組雄性子代平均體重糖水消耗量降低(p0.05)。 4.前脈沖及驚恐抑制實(shí)驗(yàn)：結(jié)果顯示在前脈沖抑制反應(yīng)中孕中期產(chǎn)前應(yīng)激組子代與對(duì)照組子代相比反應(yīng)減低(p0.05)；在驚恐反應(yīng)孕晚期產(chǎn)前應(yīng)激子代與對(duì)照組子代相比反應(yīng)增高(p0.01)。 5.強(qiáng)迫游泳實(shí)驗(yàn)：結(jié)果顯示與對(duì)照組子代相比孕晚期的產(chǎn)前應(yīng)激組雌子代(p0.05)和雄性子代(p0.01)的“絕望”行為增加,而孕中期產(chǎn)前應(yīng)激組僅雄性子代大鼠“絕望”行為增加(p0.05)。 二.背側(cè)海馬和腹側(cè)海馬的IL-18含量及MEK-1陽性細(xì)胞檢測(cè)結(jié)果： 1.IL-18結(jié)果顯示與對(duì)照組相比孕晚期產(chǎn)前應(yīng)激可導(dǎo)致雌性子代的腹側(cè)海馬IL-18含量增加(p0.05),而孕中期產(chǎn)前應(yīng)激對(duì)子代海馬IL-18含量的無影響(p0.05)。 2.MEK-1陽性細(xì)胞結(jié)果顯示與對(duì)照組相比孕晚期可以導(dǎo)致背側(cè)海馬MEK-1的表達(dá)增加(p0.01)及腹側(cè)海馬MEK-1的表達(dá)下降(p0.05),而孕中期對(duì)海馬的MEK-1的表達(dá)無影響(p0.05)。 結(jié)論 1.孕中期和孕晚期產(chǎn)期應(yīng)激均可導(dǎo)致子代行為學(xué)的改變,但孕晚期產(chǎn)前應(yīng)激對(duì)子代行為學(xué)的影響更多。 2.不同性別的產(chǎn)前應(yīng)激子代大鼠行為學(xué)改變存在差異,產(chǎn)前應(yīng)激對(duì)雄性子代的影響高于對(duì)雌性子代的影響。 3.孕晚期產(chǎn)前應(yīng)激可導(dǎo)致子代大鼠海馬的炎性因子IL-18分泌增加及MEK-1信號(hào)蛋白表達(dá)異常。 4.腹側(cè)和背側(cè)海馬的“功能分離”可能與MEK-1信號(hào)蛋白的表達(dá)及其相關(guān)的絲裂原活化蛋白激酶(Mitogen-activated protein kinases, MAPKs)信號(hào)傳導(dǎo)途徑有關(guān)。 5.IL-18可能參與MEK-1信號(hào)蛋白的表達(dá)的調(diào)控。
[Abstract]:Purpose In this study, we established animal models of pre-production stress rats in different periods, observed the behavior of pre-stress on filial generation in different periods, cytokine interleukin 18 (IL-18) and extracellular signal regulated kinase (MEK-1). Effects of pre-production stress-sensitive period and gender effect on the pathogenesis of pre-production stress-related diseases were discussed. for the purposes of According to the method, SD pregnant rats were randomly divided into 3 groups, and the control group did not give any stimulation during pregnancy; the medium-term stress group was subjected to binding stimulation from 9 to 14 days of pregnancy; and the late pregnancy group was from the first trimester of pregnancy. 5-20 days of restraint irritation. On Day 21 after birth of children The male and female rats were fed with milk and the male and female rats were fed into cage. The children were born on the 75th day after birth. The experiment of new things, sucrose favorite experiment, pre-pulse suppression and panic reflex experiment and forced swimming experiment were carried out. 5 behavioral tests were carried out and the hippocampus of rats were tested. IL -1 8 and MEK-1. Results: 1. The results of behavioral experiment: The results showed that the total distance of male offspring stimulated male offspring was decreased (P0.05) compared with the male offspring of the control group, and the total distance of the central region was decreased (p 0. 05), the central region dwell time was decreased (P0.01). 2. New things experiment: The results showed that male offspring in the middle of the pregnancy were compared to male offspring of the control group (n. The results showed that the male offspring of the control group were compared with those in the control group. The average body weight loss of male offspring in the pre-pregnant stress group was lower than that in the pre-pregnant stress group (P0.05). The results showed that the offspring of the pre-pregnant stress group and the offspring of the control group were lower than that of the control group (P0.05). The results showed that compared with the offspring of the control group, the 鈥渄espair鈥，

本文編號(hào)：2282593