【摘要】：目的研究鳥苷酸結(jié)合蛋白2(GBP2)對(duì)樹突狀細(xì)胞(DC)成熟和功能的影響。方法通過基因芯片和實(shí)時(shí)定量PCR研究β-葡聚糖誘導(dǎo)的DC基因的變化情況,轉(zhuǎn)染GBP2小干擾RNA(si RNA),敲低GBP2的表達(dá),流式細(xì)胞術(shù)檢測(cè)DC表面CD11c、主要組織相容性復(fù)合體Ⅱ(MHC-Ⅱ)、CD80水平,ELISA檢測(cè)細(xì)胞上清中白細(xì)胞介素6(IL-6)、IL-12p70、TNF-α和IL-10水平。T細(xì)胞增殖實(shí)驗(yàn)檢測(cè)全葡聚糖顆粒(WGP)刺激的成熟DC在轉(zhuǎn)染GBP2-si RNA后,對(duì)卵清蛋白(OVA)特異性CD4+T細(xì)胞的促增殖效應(yīng)。結(jié)果β-葡聚糖誘導(dǎo)后,DC的GBP2 m RNA水平顯著降低;敲低DC的GBP2水平后,其表面CD11c、主要組織相容性復(fù)合體Ⅱ(MHC-Ⅱ)、CD80水平降低,同時(shí),IL-6、IL-12、TNF-α分泌下降;經(jīng)WGP刺激的成熟DC在轉(zhuǎn)染GBP2-si RNA后,對(duì)OVA特異性CD4+T細(xì)胞的促增殖效應(yīng)降低。結(jié)論 GBP2能調(diào)控β-葡聚糖誘導(dǎo)的DC成熟,進(jìn)一步抑制T細(xì)胞的增殖。
[Abstract]:Objective to study the effects of guanylate binding protein 2 (GBP2) on (DC) maturation and function in dendritic cells. Methods the changes of DC gene induced by 尾 -glucan were studied by gene chip and real-time quantitative PCR, and the expression of GBP2 was reduced by GBP2 small interference RNA (si RNA), knockout. The levels of CD11c, major histocompatibility complex 鈪，

本文編號(hào)：2274373