發(fā)布時(shí)間：2018-10-15 12:38

【摘要】：先天性心臟病是由于胎兒時(shí)期心臟和血管結(jié)構(gòu)發(fā)育異常而導(dǎo)致的出生缺陷,其致病機(jī)制比較復(fù)雜。斑馬魚是目前生物學(xué)研究中應(yīng)用較為廣泛的模式動物。由于其胚胎透明、發(fā)育速度快等特點(diǎn),有利于進(jìn)行先天性心臟病研究。斑馬魚cloche突變型具有心內(nèi)膜缺失和心臟發(fā)育畸形的突變表型,但依然可以存活,是研究先天性心臟病的良好動物模型。轉(zhuǎn)錄組測序分析能夠從整體水平研究基因表達(dá)。對發(fā)育30hpf時(shí)期野生型斑馬魚和突變型cloche斑馬魚進(jìn)行轉(zhuǎn)錄組測序,分析基因表達(dá)情況,對差異表達(dá)基因分析所涉及的相關(guān)生物學(xué)過程和通路,從總體上揭示cloche突變伴隨的相關(guān)生物學(xué)過程和通路所發(fā)生的改變。分析發(fā)現(xiàn)新的轉(zhuǎn)錄本和轉(zhuǎn)錄區(qū)域,對斑馬魚基因組注釋信息的完善提供新的信息。差異表達(dá)的新轉(zhuǎn)錄本可為cloche突變機(jī)制研究提供更多新的線索。重點(diǎn)分析cloche13號染色體末端的缺失區(qū)域,對缺失區(qū)域范圍進(jìn)行界定,分析缺失區(qū)域基因表達(dá)情況,對缺失區(qū)域內(nèi)差異表達(dá)基因特別是lycat基因所在通路基因和互作基因的差異表達(dá)情況進(jìn)行分析,揭示cloche缺失區(qū)域基因缺失表達(dá)后所引起的其它基因表達(dá)變化情況。
[Abstract]:Congenital heart disease (CHD) is a birth defect caused by abnormal development of heart and blood vessels during fetal period. Zebrafish is a widely used model animal in biological research. Because of its transparent embryo, rapid development and other characteristics, is conducive to the study of congenital heart disease. Zebrafish cloche mutant has the mutant phenotype of endocardial deletion and cardiac deformity, but it can still survive. It is a good animal model to study congenital heart disease. Transcriptome sequencing can be used to study gene expression at the global level. The transcriptome sequencing of wild zebrafish and mutant cloche zebrafish during developmental 30hpf period was carried out to analyze the gene expression and the related biological processes and pathways involved in differential expression gene analysis. The related biological processes and pathways associated with cloche mutation were revealed in general. New transcripts and transcriptional regions were found to provide new information for the improvement of zebrafish genome annotation information. The new transcripts of differential expression may provide more new clues for the study of cloche mutation mechanism. The region of deletion at the end of chromosome cloche13 was analyzed, the region of deletion was defined, and the expression of gene in deletion region was analyzed. The differential expression of differentially expressed genes, especially the genes involved in the pathway of lycat gene and the interaction genes in the deletion region, was analyzed to reveal the changes of other genes expression caused by the gene deletion in the cloche deletion region.
【學(xué)位授予單位】：中國科學(xué)院北京基因組研究所
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R541.1;R-332

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相關(guān)期刊論文 前2條

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