【摘要】：先天性心臟病是由于胎兒時期心臟和血管結構發(fā)育異常而導致的出生缺陷,其致病機制比較復雜。斑馬魚是目前生物學研究中應用較為廣泛的模式動物。由于其胚胎透明、發(fā)育速度快等特點,有利于進行先天性心臟病研究。斑馬魚cloche突變型具有心內膜缺失和心臟發(fā)育畸形的突變表型,但依然可以存活,是研究先天性心臟病的良好動物模型。轉錄組測序分析能夠從整體水平研究基因表達。對發(fā)育30hpf時期野生型斑馬魚和突變型cloche斑馬魚進行轉錄組測序,分析基因表達情況,對差異表達基因分析所涉及的相關生物學過程和通路,從總體上揭示cloche突變伴隨的相關生物學過程和通路所發(fā)生的改變。分析發(fā)現新的轉錄本和轉錄區(qū)域,對斑馬魚基因組注釋信息的完善提供新的信息。差異表達的新轉錄本可為cloche突變機制研究提供更多新的線索。重點分析cloche13號染色體末端的缺失區(qū)域,對缺失區(qū)域范圍進行界定,分析缺失區(qū)域基因表達情況,對缺失區(qū)域內差異表達基因特別是lycat基因所在通路基因和互作基因的差異表達情況進行分析,揭示cloche缺失區(qū)域基因缺失表達后所引起的其它基因表達變化情況。
[Abstract]:Congenital heart disease (CHD) is a birth defect caused by abnormal development of heart and blood vessels during fetal period. Zebrafish is a widely used model animal in biological research. Because of its transparent embryo, rapid development and other characteristics, is conducive to the study of congenital heart disease. Zebrafish cloche mutant has the mutant phenotype of endocardial deletion and cardiac deformity, but it can still survive. It is a good animal model to study congenital heart disease. Transcriptome sequencing can be used to study gene expression at the global level. The transcriptome sequencing of wild zebrafish and mutant cloche zebrafish during developmental 30hpf period was carried out to analyze the gene expression and the related biological processes and pathways involved in differential expression gene analysis. The related biological processes and pathways associated with cloche mutation were revealed in general. New transcripts and transcriptional regions were found to provide new information for the improvement of zebrafish genome annotation information. The new transcripts of differential expression may provide more new clues for the study of cloche mutation mechanism. The region of deletion at the end of chromosome cloche13 was analyzed, the region of deletion was defined, and the expression of gene in deletion region was analyzed. The differential expression of differentially expressed genes, especially the genes involved in the pathway of lycat gene and the interaction genes in the deletion region, was analyzed to reveal the changes of other genes expression caused by the gene deletion in the cloche deletion region.
【學位授予單位】：中國科學院北京基因組研究所
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R541.1;R-332

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相關期刊論文 前2條

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