【摘要】：目的: 1.建立福氏志賀菌感染的SD大鼠模型,為進(jìn)一步研究志賀菌的致病機(jī)制和疫苗防治提供基礎(chǔ)。 2.探討兩歧雙歧桿菌對(duì)福氏志賀菌感染大鼠的治療效果。 方法: 1.先用硫酸鏈霉素給正常大鼠灌胃,出現(xiàn)菌群失調(diào)癥狀后,再通過福氏志賀菌灌胃感染大鼠,根據(jù)臨床表現(xiàn)和病理切片確定感染模型的建立。 2.使用兩歧雙歧桿菌干預(yù)治療福氏志賀菌感染大鼠模型,檢測(cè)治療組中治療期間大鼠糞便的福氏志賀菌含量變化。 3.檢測(cè)干預(yù)治療后各組大鼠血中IL-2、腸粘液中sIgA的含量。 4.用MTT法檢測(cè)雙歧桿菌干預(yù)治療對(duì)大鼠脾細(xì)胞增殖的影響，并用脾臟指數(shù)及胸腺指數(shù)評(píng)價(jià)雙歧桿菌對(duì)SD大鼠的免疫增強(qiáng)作用。 結(jié)果: 1.感染大鼠表現(xiàn)為糞質(zhì)較稀，病理切片顯示腸粘膜壞死脫落，表明感染模型建立成功。 2.雙歧桿菌治療后，糞便中志賀菌含量下降迅速。 3.與未干預(yù)組和生理鹽水組相比雙歧桿菌治療組IL-2、腸粘液中sIgA的水平明顯升高。 4. MTT法表明雙歧桿菌治療組志賀菌抗原刺激的脾細(xì)胞增殖水平顯著高于未治療組。 結(jié)論: 1.成功建立了福氏志賀菌感染的大鼠模型。 2.兩歧雙歧桿菌治療能使感染模型糞便中志賀菌含量迅速下降。 3.兩歧雙歧桿菌對(duì)福氏志賀菌感染機(jī)體的IL-2、sIgA分泌有促進(jìn)作用。 4.雙歧桿菌能促進(jìn)感染機(jī)體的脾細(xì)胞增殖。
[Abstract]:Objective: 1. The SD rat model of Shigella flexneri infection was established to provide a basis for further study on the pathogenic mechanism and vaccine control of Shigella flexneri. To investigate the therapeutic effect of bifidobacterium on rats infected with Shigella flexneri. Methods: 1. The normal rats were given streptomycin sulfate first and then infected by Shigella flexneri. The infection model was established according to the clinical manifestations and pathological sections. 2. The rat model of Shigella flexneri infection was treated with bifidobacterium. The content of Shigella flexneri in feces of rats in the treatment group was measured during the treatment period. 3. The content of sIgA in IL-2, intestinal mucus of rats in each group after intervention was detected. 4. 4. The effects of Bifidobacterium on the proliferation of spleen cells in rats were detected by MTT method. The immune enhancement of SD rats was evaluated by spleen index and thymus index. Results: 1. The pathological sections showed intestinal mucosal necrosis and shedding, indicating that the infection model was established successfully. 2. After Bifidobacterium treatment, Shigella content in feces decreased rapidly. The level of sIgA in intestinal mucus of IL-2, in Bifidobacterium treated group was significantly higher than that in non-intervention group and normal saline group. 4. 4. MTT assay showed that the proliferation level of spleen cells stimulated by Shigella antigen in Bifidobacterium treated group was significantly higher than that in untreated group. Conclusion: 1. The rat model of Shigella flexneri infection was established successfully. Bifidobacterium bifidobacterium treatment can rapidly reduce Shigella content in feces of infected model. Bifidobacterium can promote the secretion of IL-2,sIgA in Shigella flexneri infection. 4. Bifidobacterium can promote the proliferation of spleen cells infected with the body.
【學(xué)位授予單位】：大理學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R-332

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