【摘要】：目的： 應用DiversiLab系統(tǒng)對鮑曼不動桿菌進行基因分型,并系統(tǒng)評價DiversiLab系統(tǒng)、多位點序列分析技術、脈沖場凝膠電泳技術的分辨率和三種方法的一致性分析。 方法： 對2005年全國15個城市醫(yī)院臨床分離的89株鮑曼不動桿菌,應用以rep-PCR為原理的DiversiLab系統(tǒng)分型技術進行分型,并與脈沖場凝膠電泳分型和多位點序列分析技術結果進行比較；應用Ridom EpiCompare Version 1.0軟件得出Simpson相關系數(shù)及可信區(qū)間、AR系數(shù)和Wallace系數(shù)等,從而更精確地比較三種方法的分辨力和一致性分析。 結果： DiversiLab系統(tǒng)將89株鮑曼不動桿菌分為8簇流行克隆和28個散發(fā)克��；多位點序列分型將其分為39個序列型,并歸為一個克隆復合體CC92 (Clone Complex 92)和47個單一體(Singleton);脈沖場凝膠電泳將其分成5個流行脈沖型和37個散發(fā)克隆。DiversiLab系統(tǒng)、MLST和PFGE的Simpson相關系數(shù)分別為0.899,0.894和0.934。AR (MLST-PFGE)=0.502, AR (DiversiLab-PFGE) =0.479, AR (DiversiLab-MLST)=0.434,從AR值來看,MLST和PFGE的相關性最好。對于DiversiLab系統(tǒng)而言,與PFGE分型結果的一致性比其與MLST分型結果的一致性好。Wallace系數(shù)中,W1 (PFGE-MLST)=0.709, W1 (PFGE-DiversiLab)=0.659。因此,可由PFGE結果大致推測出MLST和DiversiLab系統(tǒng)分型結果。 結論： 在鮑曼不動桿菌的分子分型方法中,DiversiLab系統(tǒng)具備較高的分辨力和準確性,與PFGE分型結果的一致性比與MLST的一致性好；且其操作快速、簡單,可重復性好,可用于快速或大量菌株的首選分型方法。
[Abstract]:Objective: to study the genotyping of Acinetobacter baumannii by DiversiLab system, and to evaluate the resolution of DiversiLab system, multi-locus sequence analysis, pulse field gel electrophoresis and consistency analysis of three methods. Methods: 89 strains of Acinetobacter baumannii isolated from 15 hospitals in 2005 were classified by DiversiLab system based on rep-PCR. The results were compared with the results of pulse field gel electrophoresis typing and multilocus sequence analysis, and the Simpson correlation coefficient and confidence interval, AR coefficient and Wallace coefficient were obtained by Ridom EpiCompare Version 1.0 software. Thus, the resolution and consistency analysis of the three methods are compared more accurately. Results: 89 strains of Acinetobacter baumannii were divided into 8 clusters of epidemic clones and 28 sporadic clones by DiversiLab system. A clone complex CC92 (Clone Complex 92) and 47 monosomes (Singleton); pulse field gel electrophoresis were divided into 5 popular pulse types and 37 sporadic clones. The Simpson correlation coefficients of MLST and PFGE were 0.899N0.894 and 0.502, AR (DiversiLab-PFGE) = 0.479, AR (DiversiLab-MLST) = 0.434, respectively. In terms of AR, MLST and PFGE have the best correlation. For DiversiLab system, the consistency with PFGE typing results is better than that with MLST typing results. In Wallace coefficient, W1 (PFGE-MLST) = 0.709, W1 (PFGE-DiversiLab) = 0.659. Therefore, the results of MLST and DiversiLab system typing can be inferred from PFGE results. Conclusion: among the molecular typing methods of Acinetobacter baumannii, the DiversiLab system has higher resolution and accuracy, better consistency with the results of PFGE typing than with MLST, and its operation is fast, simple and reproducible. It can be used as the preferred typing method for rapid or large number of strains.
【學位授予單位】：浙江大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R378

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