大鼠恐懼應(yīng)激模型中行為與心血管指標變化
發(fā)布時間：2018-09-10 08:59
【摘要】：目的:建立條件恐懼應(yīng)激致大鼠心血管損傷防治藥物藥效評價實驗動物模型,觀察恐懼應(yīng)激對大鼠心血管功能的損傷效應(yīng),并對心血管損傷相關(guān)備選指標進行綜合的檢測、分析、評價。篩選有意義的評價抗恐懼藥物藥效備選指標,以備今后建立評價體系使用。 方法: 64只健康雄性wistar大鼠隨機分為恐懼模型組與對照組,每組32只。自由攝食飲水飼養(yǎng)10天后,第11天起恐懼模型組每日給予2s噪音+3s足底電流刺激,電流強度為1mA,間隔1min,共30次,第12、13天重復(fù)實驗;對照組每日只給予2s噪音,無足底電流刺激,間隔1min,共30次,第12、13天重復(fù)實驗。第14天攝像記錄兩組大鼠5min噪音之后第6—30min內(nèi)行為變化,評定造模是否成功。自造模成功之后第二日算起分別于1、2、3、4周每周周中(第18、25、32、39天),對兩組大鼠進行體重、自發(fā)活動、血壓心率以及大鼠血清高敏C反應(yīng)蛋白、肌酸激酶同工酶、心肌鈣蛋白I含量測定。 結(jié)果: (1)第14天攝像觀察兩組大鼠5min噪音之后行為學(xué)反應(yīng),采用三人評定法,恐懼模型組凍結(jié)時間顯著高于對照組,差異有統(tǒng)計學(xué)意義(P0.05),證明造模成功。(2)兩組大鼠體重均稱自然增長態(tài)勢,第1周兩組大鼠體重?zé)o顯著性差異,第2周(P0.05)、3周(P0.05)、4周(P0.01)兩組大鼠體重比較均差異顯著。(3)兩組大鼠自發(fā)活動比較,恐懼模型組活動次數(shù)在第1周(P0.05)、2周(P0.01)、3周(P0.01)、4周(P0.05)均顯著少于對照組,差異顯著。(4)兩組大鼠血壓值比較,恐懼模型組大鼠血壓趨勢呈持續(xù)升高,對照組無明顯變化。第1周兩組大鼠血壓無顯著性差異,第2周(P0.05)、3周(P0.01)、4周(P0.01)兩組大鼠血壓比較均差異顯著。(5)兩組大鼠心率比較,第1周兩組大鼠心率無顯著性差異,第2周(P0.05)、3周(P0.05)、4周(P0.01)兩組大鼠心率比較均差異顯著。(6)兩組大鼠血清C反應(yīng)蛋白含量比較,恐懼模型組在第1周(P0.05)、2周(P0.01)、3周(P0.01)、4周(P0.01)均顯著高于對照組;(7)兩組大鼠血清肌酸激酶同工酶含量比較,恐懼模型組大鼠血清肌酸激酶同工酶在第1、2、3周均無顯著差異,第4周(P0.01)顯著高于對照組,差異有統(tǒng)計學(xué)意義。(8)兩組大鼠血清心肌鈣蛋白I含量比較,恐懼模型組大鼠心肌鈣蛋白I含量在第1周(P0.05)、2周(P0.01)、3周(P0.01)、4周(P0.01)均顯著高于對照組,差異有統(tǒng)計學(xué)意義。 結(jié)論:本研究結(jié)果表明經(jīng)過改良的Takashi法可以成功的制造大鼠恐懼應(yīng)激模型�？謶謶�(yīng)激可以提高大鼠應(yīng)激水平進而影響大鼠的體重、血壓、心率、行為以及心血管指標的改變。部分實驗備選指標可以為抗恐懼應(yīng)激藥物藥效評價提供相關(guān)依據(jù)。
[Abstract]:Objective: to establish an experimental animal model of cardiovascular injury prevention and control drugs induced by conditioned fear stress in rats, and to observe the effects of fear stress on cardiovascular function in rats. Analysis, evaluation. To evaluate the efficacy of anti-fear drugs in order to establish an evaluation system in the future. Methods: 64 healthy male wistar rats were randomly divided into fear model group (n = 32) and control group (n = 32). After feeding free drinking water for 10 days, the fear model group was given 2s noise 3s foot current stimulation every day, the current intensity was 1 Ma, the interval was 30 times, the 1213 days repeated experiment, the control group was only given 2s noise daily. There was no plantar current stimulation, the interval was 1 min, 30 times. The experiment was repeated on the 12th day. On the 14th day, the changes of 6-30min behavior were recorded after 5min noise in the two groups, and the success of the model was evaluated. From the second day after the success of the model, the rats were given body weight, spontaneous activity, blood pressure and heart rate, serum Gao Min C-reactive protein and creatine kinase isozyme in the second day of the second day, respectively. Cardiac troponin I content was measured. Results: (1) on the 14th day, the behavioral responses after 5min noise in the two groups were observed. The freezing time of the fear model group was significantly longer than that of the control group. The difference was statistically significant (P0.05), which proved that the model was successful. (2) the body weight of the two groups were all called natural growth trend, and there was no significant difference in the body weight between the two groups at the first week. There were significant differences in body weight between the two groups at the 2nd week (P0.05) and the 4th week (P0.01). (3) the activity times of the fear model group were significantly lower than those of the control group at the 1st week (P0.05) and the 2nd week (P0.01) and the 4th week (P0.05), the difference was significant. (4) the blood pressure value of the two groups was significantly lower than that of the control group. The trend of blood pressure in the fear model group was continuously increased, but that in the control group was not significantly changed. In the first week, there was no significant difference in blood pressure between the two groups, but in the second week (P0.05) and the third week (P0.01) and the fourth week (P0.01), there were significant differences in blood pressure between the two groups. (5) there was no significant difference in heart rate between the two groups in the first week, and in the first week, there was no significant difference in the heart rate between the two groups. There were significant differences in heart rate between the two groups in the 2nd week (P0.05) and the 3rd week (P0.05) and the 4th week (P0.01). (6) the content of serum C-reactive protein in the two groups was higher than that in the control group. The level of serum creatine kinase isoenzyme in the fear model group was significantly higher than that in the control group at the 1st week (P0.05) and the 2nd week (P0.01) and the 4th week (P0.01) (P0.01). (7) there was no significant difference in serum creatine kinase isoenzyme content between the two groups. At the 4th week (P0.01), the difference was statistically significant. (8) compared with the control group, the level of cardiac troponin I in the fear model group was significantly higher than that in the control group at the 1st week (P0.05) and the 2nd week (P0.01) and the 3rd week (P0.01) and the 4th week (P0.01). The difference is statistically significant. Conclusion: the results of this study suggest that the modified Takashi method can successfully establish the rat model of fear stress. Fear stress can increase the stress level of rats and then affect the changes of body weight, blood pressure, heart rate, behavior and cardiovascular indexes. Some of the experimental alternative indicators can provide relevant evidence for the evaluation of the efficacy of anti-fear stress drugs.
【學(xué)位授予單位】：第二軍醫(yī)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R-332
本文編號：2233982
[Abstract]:Objective: to establish an experimental animal model of cardiovascular injury prevention and control drugs induced by conditioned fear stress in rats, and to observe the effects of fear stress on cardiovascular function in rats. Analysis, evaluation. To evaluate the efficacy of anti-fear drugs in order to establish an evaluation system in the future. Methods: 64 healthy male wistar rats were randomly divided into fear model group (n = 32) and control group (n = 32). After feeding free drinking water for 10 days, the fear model group was given 2s noise 3s foot current stimulation every day, the current intensity was 1 Ma, the interval was 30 times, the 1213 days repeated experiment, the control group was only given 2s noise daily. There was no plantar current stimulation, the interval was 1 min, 30 times. The experiment was repeated on the 12th day. On the 14th day, the changes of 6-30min behavior were recorded after 5min noise in the two groups, and the success of the model was evaluated. From the second day after the success of the model, the rats were given body weight, spontaneous activity, blood pressure and heart rate, serum Gao Min C-reactive protein and creatine kinase isozyme in the second day of the second day, respectively. Cardiac troponin I content was measured. Results: (1) on the 14th day, the behavioral responses after 5min noise in the two groups were observed. The freezing time of the fear model group was significantly longer than that of the control group. The difference was statistically significant (P0.05), which proved that the model was successful. (2) the body weight of the two groups were all called natural growth trend, and there was no significant difference in the body weight between the two groups at the first week. There were significant differences in body weight between the two groups at the 2nd week (P0.05) and the 4th week (P0.01). (3) the activity times of the fear model group were significantly lower than those of the control group at the 1st week (P0.05) and the 2nd week (P0.01) and the 4th week (P0.05), the difference was significant. (4) the blood pressure value of the two groups was significantly lower than that of the control group. The trend of blood pressure in the fear model group was continuously increased, but that in the control group was not significantly changed. In the first week, there was no significant difference in blood pressure between the two groups, but in the second week (P0.05) and the third week (P0.01) and the fourth week (P0.01), there were significant differences in blood pressure between the two groups. (5) there was no significant difference in heart rate between the two groups in the first week, and in the first week, there was no significant difference in the heart rate between the two groups. There were significant differences in heart rate between the two groups in the 2nd week (P0.05) and the 3rd week (P0.05) and the 4th week (P0.01). (6) the content of serum C-reactive protein in the two groups was higher than that in the control group. The level of serum creatine kinase isoenzyme in the fear model group was significantly higher than that in the control group at the 1st week (P0.05) and the 2nd week (P0.01) and the 4th week (P0.01) (P0.01). (7) there was no significant difference in serum creatine kinase isoenzyme content between the two groups. At the 4th week (P0.01), the difference was statistically significant. (8) compared with the control group, the level of cardiac troponin I in the fear model group was significantly higher than that in the control group at the 1st week (P0.05) and the 2nd week (P0.01) and the 3rd week (P0.01) and the 4th week (P0.01). The difference is statistically significant. Conclusion: the results of this study suggest that the modified Takashi method can successfully establish the rat model of fear stress. Fear stress can increase the stress level of rats and then affect the changes of body weight, blood pressure, heart rate, behavior and cardiovascular indexes. Some of the experimental alternative indicators can provide relevant evidence for the evaluation of the efficacy of anti-fear stress drugs.
【學(xué)位授予單位】：第二軍醫(yī)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R-332
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