【摘要】：【目的】本研究通過構(gòu)建大鼠高脂結(jié)構(gòu)模型來探究一株植物乳桿菌Lp3的益生作用�！痉椒ā恐参锶闂U菌Lp3篩選自青藏高原地區(qū)傳統(tǒng)發(fā)酵的牦牛酸奶,初步認(rèn)定Lp3是一株具有良好耐受力的降膽固醇菌株,且體外益生特性突出,本研究通過建立高脂SD大鼠模型,在飼喂試驗(yàn)動(dòng)物高脂飼料的同時(shí)灌胃植物乳桿菌Lp3,來確定該菌株對試驗(yàn)動(dòng)物血脂的影響效果,并同時(shí)研究其對大鼠腸道菌群、糞便水分、糞便中膽固醇和膽汁酸含量的影響,以及對肝臟組織中的膽固醇(TC)和甘油三酯(TG)的影響�！窘Y(jié)果】結(jié)果表明,植物乳桿菌Lp3對大鼠沒有任何明顯的毒副作用,對高脂模型大鼠具有良好的降血脂效果。飼喂高脂飼料并灌喂乳酸菌Lp3組大鼠(HL)的血清總膽固醇、甘油三酯和低密度脂蛋白膽固醇含量較飼喂高脂飼料組(HC)顯著減少(P0.05),但是高密度脂蛋白膽固醇的含量變化并不明顯。HC組大鼠與HL組及飼喂普通日糧組(對照組)大鼠相比較,HC組大鼠糞便中大腸桿菌數(shù)量明顯增加,雙歧桿菌、乳桿菌數(shù)量明顯減少。但是灌胃乳酸菌的HL組大鼠的糞便中乳桿菌數(shù)略高于對照組,大腸桿菌和雙歧桿菌數(shù)量和對照組大鼠的基本一致。表明植物乳桿菌Lp3具有維持腸道菌群平衡的作用。此外灌胃乳酸菌后HL組大鼠糞便含水量比HC組要高6.44%。HC組大鼠肝臟組織中膽固醇和甘油三酯要顯著高于HL組(P0.05),說明Lp3可以減少脂類物質(zhì)在肝臟組織中的沉積。從肝臟組織切片來看,也可以得出上述結(jié)論�！窘Y(jié)論】結(jié)果表明本研究所篩選的植物乳桿菌Lp3對高脂大鼠具有值得深入研究的益生作用。
[Abstract]:[objective] to study the probiotic effect of a Lactobacillus plantarum Lp3 strain by establishing a rat hyperlipidemia model. [methods] Lactobacillus plantarum Lp3 was used to screen yak yoghurt from Qinghai-Tibet Plateau. It was preliminarily concluded that Lp3 was a cholesterol-lowering strain with good tolerance, and its probiotic characteristics were prominent in vitro. In this study, a rat model of high-fat SD was established. In order to determine the effect of Lactobacillus plantarum Lp3, on blood lipids, the effects of Lactobacillus plantarum Lp3, on intestinal flora, fecal moisture, cholesterol and bile acid in rats were studied. The results showed that Lactobacillus plantarum Lp3 had no obvious side effects on rats, and had a good effect on reducing blood lipid in hyperlipidemic rats. The serum total cholesterol of rats fed with high fat diet and fed with lactic acid bacteria (Lp3) group was compared with that of (HL) group. The contents of triglyceride and low density lipoprotein cholesterol were significantly lower than those of high fat diet group (P0.05), but the content of high density lipoprotein cholesterol did not change significantly. HC group, HL group and general diet group (control group). The number of Escherichia coli in feces of rats in HC group was significantly higher than that in control group. The number of Bifidobacterium and Lactobacillus decreased significantly. However, the number of lactobacillus in feces of HL group was slightly higher than that of control group, and the number of Escherichia coli and Bifidobacterium was basically the same as that of control group. The results showed that Lactobacillus plantarum Lp3 could maintain the balance of intestinal flora. In addition, the fecal water content of HL group was significantly higher than that of HC group compared with HC group (P0.05), indicating that Lp3 could reduce lipid deposition in liver tissue. Conclusion: the results showed that Lactobacillus plantarum Lp3 screened in this study had a prebiotic effect on hyperlipidemic rats.
【作者單位】： 蘭州大學(xué)生命科學(xué)學(xué)院草地農(nóng)業(yè)生態(tài)系統(tǒng)國家重點(diǎn)實(shí)驗(yàn)室;蘭州生物制品研究所;
【基金】：國家自然科學(xué)基金(31272486)~~
【分類號】：R-332;R589.2

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