發(fā)布時(shí)間：2018-09-04 12:28

【摘要】：目的:研究海德爾堡沙門氏菌(Salmonella heidelberg)低pH值酸適應(yīng)性及酸適應(yīng)對細(xì)胞其他脅迫環(huán)境耐受性的影響。方法:海德爾堡沙門氏菌在pH 5.5酸性培養(yǎng)基中生長2h誘導(dǎo)酸適應(yīng)性,以pH 3.0酸脅迫培養(yǎng)基及不同溫度、NaCl、膽鹽、消毒劑環(huán)境的存活率評(píng)價(jià)其耐受性。結(jié)果:pH 5.5酸適應(yīng)2 h后,海德爾堡沙門氏菌在pH 3.0酸脅迫環(huán)境的存活率大幅提高。低pH值酸適應(yīng)也使海德爾堡沙門氏菌在45、50℃高溫條件下的存活率顯著提高,對4℃冷藏環(huán)境的耐受性顯著低于非酸處理菌株,但對-20℃冷凍環(huán)境的生存無影響。酸適應(yīng)海德爾堡沙門氏菌對高滲、膽鹽脅迫也表現(xiàn)良好的耐受性,5~10 g/100mL NaCl溶液處理1 h,酸適應(yīng)海德爾堡沙門氏菌的存活率大于60%,在1~10 g/100mL膽鹽溶液的存活率比對照組高3.8倍。海德爾堡沙門氏菌酸適應(yīng)性也使其對消毒劑的敏感性發(fā)生變化,酸適應(yīng)性海德爾堡沙門氏菌在10~25 mmol/LH_2O_2溶液的存活率顯著高于對照菌株,最高達(dá)3.1倍;但對NaClO、乙醇消毒劑的耐受性顯著降低,高質(zhì)量濃度下二者無顯著差異。結(jié)論:海德爾堡沙門氏菌在低pH值酸性條件生長一段時(shí)間會(huì)誘導(dǎo)產(chǎn)生酸適應(yīng)性,還會(huì)協(xié)同提高其對高溫、高滲、膽鹽、H_2O_2脅迫環(huán)境的交叉抗性;但對4、-20℃低溫、NaClO、乙醇等殺菌、保藏措施敏感性提高。
[Abstract]:Aim: to study the low pH acid adaptability of (Salmonella heidelberg) and the effect of acid adaptation on the tolerance of other stress environments of Salmonella heidelliensis. Methods: the acid adaptability of Salmonella Heidelberg was induced in pH 5.5 acid medium for 2 h. The tolerance was evaluated by the survival rate of pH 3.0 acid stress medium and NaCll, bile salt and disinfectants at different temperatures. Results the survival rate of Salmonella Heidelberg under acid stress of pH 3. 0 was significantly increased after 5. 5% acid acclimation for 2 h. Low pH acid adaptation also increased the survival rate of Salmonella Heidelberg at 45 鈩，

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