【摘要】：目的研究TRL4-NOD2(T4N2)信號(hào)傳遞增強(qiáng)樹突狀細(xì)胞(dendritic cell,DC)抗結(jié)核分枝桿菌(MTB)感染機(jī)制,為結(jié)核病(tuberculosis,TB)的免疫防治提供參考。方法分別用TLR4配體LPS、NOD2配體MDP和T4N2雙配體刺激DC后,ELISA法定量檢測(cè)1h、6h、12h、24h和48h上清液中IL-6和IL-12。DC經(jīng)MTB感染再用LPS、MDP以及T4N2雙配體刺激,24h后分別收集細(xì)胞培養(yǎng)并計(jì)數(shù)細(xì)胞內(nèi)生長(zhǎng)的菌落數(shù),判斷細(xì)菌生長(zhǎng)的抑制率。結(jié)果ELISA顯示T4N2雙信號(hào)刺激DC后,細(xì)胞因子IL-6在1h、6h、12h、24h和48h濃度分別為(86.4±0.34)pg/ml、(92.2±0.69)pg/ml、(93.6±0.69)pg/ml、(96.0±1.73)pg/ml和(107.8±4.53)pg/ml;IL-12在1h、6h、12h、24h和48h濃度分別為(94.6±4.07)pg/ml、(100.9±4.45)pg/ml、(97.9±2.96)pg/ml、(112.4±9.28)pg/ml和(132.8±1.49)pg/ml。不同時(shí)間段T4N2雙信號(hào)刺激較LPS、MDP刺激顯著增多(P0.05);T4N2雙信號(hào)活化的DC對(duì)MTB生長(zhǎng)抑制率顯著增強(qiáng)。結(jié)論 T4N2信號(hào)傳遞能協(xié)同增強(qiáng)DC的活化,活化的DC可抑制MTB的生長(zhǎng)。
[Abstract]:Objective to study the mechanism of TRL4-NOD2 (T4N2) signal transduction enhanced dendritic cells (dendritic cell,DC) against (MTB) infection of Mycobacterium tuberculosis and to provide a reference for the prevention and treatment of tuberculosis (tuberculosis,TB). Methods TLR4 ligand LPS,NOD2 ligand MDP and T4N2 double ligand stimulated DC were used to detect the number of cells cultured in the supernatant of IL-6 and IL-12.DC after MTB infection and LPS,MDP and T4N2 double ligand stimulation for 24 h and 48 h, respectively. To judge the inhibition rate of bacteria growth. 緇撴灉ELISA鏄劇ずT4N2鍙屼俊鍙峰埡嬋，

本文編號(hào)：2212778