【摘要】：目的研究TRL4-NOD2(T4N2)信號傳遞增強樹突狀細胞(dendritic cell,DC)抗結(jié)核分枝桿菌(MTB)感染機制,為結(jié)核病(tuberculosis,TB)的免疫防治提供參考。方法分別用TLR4配體LPS、NOD2配體MDP和T4N2雙配體刺激DC后,ELISA法定量檢測1h、6h、12h、24h和48h上清液中IL-6和IL-12。DC經(jīng)MTB感染再用LPS、MDP以及T4N2雙配體刺激,24h后分別收集細胞培養(yǎng)并計數(shù)細胞內(nèi)生長的菌落數(shù),判斷細菌生長的抑制率。結(jié)果ELISA顯示T4N2雙信號刺激DC后,細胞因子IL-6在1h、6h、12h、24h和48h濃度分別為(86.4±0.34)pg/ml、(92.2±0.69)pg/ml、(93.6±0.69)pg/ml、(96.0±1.73)pg/ml和(107.8±4.53)pg/ml;IL-12在1h、6h、12h、24h和48h濃度分別為(94.6±4.07)pg/ml、(100.9±4.45)pg/ml、(97.9±2.96)pg/ml、(112.4±9.28)pg/ml和(132.8±1.49)pg/ml。不同時間段T4N2雙信號刺激較LPS、MDP刺激顯著增多(P0.05);T4N2雙信號活化的DC對MTB生長抑制率顯著增強。結(jié)論 T4N2信號傳遞能協(xié)同增強DC的活化,活化的DC可抑制MTB的生長。
[Abstract]:Objective to study the mechanism of TRL4-NOD2 (T4N2) signal transduction enhanced dendritic cells (dendritic cell,DC) against (MTB) infection of Mycobacterium tuberculosis and to provide a reference for the prevention and treatment of tuberculosis (tuberculosis,TB). Methods TLR4 ligand LPS,NOD2 ligand MDP and T4N2 double ligand stimulated DC were used to detect the number of cells cultured in the supernatant of IL-6 and IL-12.DC after MTB infection and LPS,MDP and T4N2 double ligand stimulation for 24 h and 48 h, respectively. To judge the inhibition rate of bacteria growth. 緇撴灉ELISA鏄劇ずT4N2鍙屼俊鍙峰埡嬋，

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