血清放置時間對大鼠血生化檢測的影響
發(fā)布時間:2018-08-29 15:32
【摘要】:目的探討大鼠血清放置時間對14項血生化檢測結(jié)果的影響。方法隨機選取40只成年SD大鼠,空腹采集靜脈血液標本,靜置1 h,分離血清并密封分裝,存放于4℃和-20℃冰箱內(nèi),應(yīng)用全自動生化分析儀于分離血清后0 h、4 h、24 h、96 h、7 d檢測谷丙轉(zhuǎn)氨酶(ALT)、谷草轉(zhuǎn)氨酶(AST)、堿性磷酸酶(ALP)、總蛋白(TP)、白蛋白(ALB)、肌酐(CREA-J)、尿酸(UA)、尿素氮(UREA)、血糖(GLU)、總膽固醇(TC)、甘油三酯(TG)、低密度脂蛋白膽固醇(LDL-C)、肌酸激酶(CK)和乳酸脫氫酶(LDH)等14項血生化指標,并進行比較。結(jié)果雌雄大鼠血生化數(shù)據(jù)變化趨勢一致。4℃保存時,與0 h相比,ALP在4 h、24 h、96 h、7d均顯著降低(P0.05),ALB在96 h和7 d均極顯著升高(P0.01),CREA-J在96 h和7 d均顯著升高(P0.05),UA在24 h、96 h、7 d均極顯著升高(P0.01),其他指標差異無顯著性(P0.05)。-20℃保存時,與0 h相比,ALT在7 d顯著升高(P0.01),AST在96 h和7 d顯著升高(P0.05),TP在4 h和24 h顯著降低(P0.05),ALB在4 h、24 h、96 h、7 d均顯著升高(P0.01),CREA-J在24 h、96 h、7 d均顯著升高(P0.01),UA在4 h、24 h、96 h、7 d均顯著升高(P0.01),TC在4 h、24 h、96 h、7 d均顯著升高(P0.05),TG在96 h和7 d顯著升高(P0.05),CK在96 h和7 d顯著升高(P0.05),LDH在96 h和7 d顯著升高(P0.05),其他指標差異無顯著性(P0.05)。結(jié)論大鼠血生化測定盡可能在采集血清后即刻完成,尤其是ALP的測定,4℃冷藏時,測定UA不宜超過4 h,測定ALB和CREA-J不宜超過24 h,測定ALT、AST、TP、UREA、GLU、TC、TG、LDL-C、CK及LDH等指標不宜超過7 d。
[Abstract]:Objective to investigate the effect of serum storage time on the results of 14 blood biochemical tests in rats. Methods Forty adult SD rats were randomly selected. Venous blood samples were collected on an empty stomach for 1 hour. The serum was separated and sealed and stored in refrigerator at 4 鈩,
本文編號:2211633
[Abstract]:Objective to investigate the effect of serum storage time on the results of 14 blood biochemical tests in rats. Methods Forty adult SD rats were randomly selected. Venous blood samples were collected on an empty stomach for 1 hour. The serum was separated and sealed and stored in refrigerator at 4 鈩,
本文編號:2211633
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