【摘要】：目的觀察小鼠卵泡發(fā)育過程中縫隙連接蛋白43(Cx43)和黃體生成素受體(LHR)在小鼠卵巢中的表達以及黃體生成素(LH)對卵巢Cx43表達的影響,為這兩種分子與卵泡發(fā)育的關系提供實驗依據。方法選擇出生后1d、4d、7d、2周、3周、4周、6周、8周齡雌性C57小鼠共8組,每組10只,取卵巢。HE染色觀察卵巢形態(tài)、卵泡發(fā)育并測量顆粒細胞體積分數。免疫組織化學和Western blotting觀察卵巢組織中Cx43及LHR的表達,Western blotting檢測不同濃度黃體生成素(LH)孵育后對卵巢Cx43表達的影響。結果 HE染色顯示,出生1d小鼠卵巢中見原始卵泡,出生7d卵巢內出現初級卵泡,2周時出現竇前卵泡和少量竇卵泡,3周、4周時,出現較多大的竇卵泡,6~8周時出現黃體。卵泡顆粒細胞體積分數在2周后的卵巢中明顯增加。Cx43表達在各階段卵泡的顆粒細胞胞膜上,LHR表達在卵泡膜細胞、顆粒細胞及卵母細胞胞質中。Cx43和LHR出生后2周卵巢中的表達開始明顯增加,并隨卵泡發(fā)育表達逐漸增強,在3周、4周、6周和8周維持相對較高水平。LH體外干預可增加卵巢組織Cx43的表達。結論 Cx43和LHR在卵泡發(fā)育中發(fā)揮重要作用,LH可能通過LHR促進卵巢卵泡顆粒細胞Cx43的表達。
[Abstract]:Objective to observe the expression of gap junction protein 43 (Cx43) and luteinizing hormone receptor (LHR) in mouse ovary during follicular development and the effect of luteinizing hormone (LH) on the expression of Cx43 in ovary, and to provide experimental evidence for the relationship between these two molecules and follicular development. Methods A total of 10 female C57 mice (10 in each group) were divided into 8 groups, including 10 female C57 mice at the age of 8 weeks, 1 day and 4 days and 7 days after birth. Ovarian morphology, follicle development and granulosa cell volume fraction were observed with HE staining. Immunohistochemistry and Western blotting were used to detect the expression of Cx43 and LHR in ovarian tissue. Western blotting was used to detect the effect of different concentrations of luteinizing hormone (LH) on the expression of Cx43 in ovary. Results HE staining showed that primordial follicles were found in the ovaries of 1 day old mice, preantral follicles were found in the ovary of 7 days after birth, preantral follicles appeared in 2 weeks and a few antral follicles appeared in 4 weeks, and more large antral follicles appeared in corpus luteum at 68 weeks after birth. The volume fraction of follicular granulosa cells in the ovary increased significantly after 2 weeks. Cx43 was expressed on the membrane of granulosa cells in all stages of follicles, and LHR was expressed in follicular membrane cells. The expression of. Cx43 and LHR in the cytoplasm of granulosa cells and oocytes began to increase 2 weeks after birth, and increased gradually with follicular development. At 3 weeks, 4 weeks, 6 weeks and 8 weeks, the level of LH was relatively high. LH increased the expression of Cx43 in ovarian tissues. Conclusion Cx43 and LHR play an important role in follicular development. LH may promote the expression of Cx43 in follicular granulosa cells through LHR.
【作者單位】： 寧夏醫(yī)科大學基礎醫(yī)學院人體解剖學與組織學胚胎學系 生育力保持教育部重點實驗室 寧夏生殖與遺傳重點實驗室;
【基金】：國家自然科學基金(81460230,81460644,81160084)
【分類號】：R321

【相似文獻】

相關期刊論文 前3條

1 馬如煥;人類卵巢濾泡及黃體之促黃體生成素受體[J];國外醫(yī)學(計劃生育分冊);1982年02期

2 時宇，史凌云，梁克珊，張志文;RNase保護法同時檢測黃體生成素受體及17α－羥化酶的mRNA[J];生物化學與生物物理進展;1995年05期

3 ;[J];;年期

相關會議論文 前1條

1 柯江維;王建紅;段榮;;心理應激雌性大鼠卵巢促卵泡刺激素受體及黃體生成素受體的變化[A];華東六省一市生物化學與分子生物學學會2006年學術交流會論文集[C];2006年

，

本文編號：2197038