【摘要】：目的:通過注射鏈脲佐菌素(STZ)制備大鼠糖尿病眼病模型,探討三七多糖對糖尿病模型大鼠的降血糖作用及其眼病的治療作用,闡明其作用機制。方法:70只SD雄性大鼠分為對照組(10只)和造模組(60只),造模組大鼠給予高脂飼料喂養(yǎng)。2周后,造模組大鼠腹腔注射35mg·kg~(-1)STZ制備高血糖模型。3d后,動物禁食不禁水12h,檢測空腹血糖(FBG)水平,以FBG11.1mmol·L-1為造模成功標準。選取造模成功的大鼠,隨機分為模型組,二甲雙胍(150 mg·kg~(-1))組,低、中、高劑量(75、150和300 mg·kg~(-1))三七多糖組。灌胃給藥5和8周后檢測大鼠FBG水平,8周后檢測大鼠糖耐量,處死大鼠取肝臟檢測肝糖原水平,檢測血清谷胱甘肽(GSH)和一氧化氮(NO)水平。分離大鼠視網膜,Q-PCR法檢測血管內皮生長因子(VEGF)及誘導型一氧化氮合酶(iNOS)的表達水平,HE染色觀察其組織形態(tài)表現。結果:與模型組比較,用藥5周后,中劑量三七多糖組大鼠FBG水平明顯降低(P0.05);用藥8周后,不同劑量三七多糖組大鼠FBG、糖耐量和肝糖原水平明顯降低(P0.05或P0.01)。血清檢測,與模型組比較,高劑量三七多糖組大鼠GSH水平明顯升高,NO水平明顯降低(P0.05或P0.01)。Q-PCR法檢測,與模型組比較,高劑量三七多糖組大鼠視網膜中VEGF和iNOS表達水平明顯降低(P0.05)。HE染色,與模型組比較,中和低劑量三七多糖組大鼠視網膜部分血管增生,未見視網膜萎縮;高劑量三七多糖組大鼠視網膜血管增生狀態(tài)明顯改善,未見視網膜萎縮。結論:三七多糖具有降血糖的作用,同時可以通過升高GSH和NO水平、提高VEGF和iNOS基因表達水平,起到治療糖尿病引起的視網膜病變的作用。
[Abstract]:Aim: to investigate the hypoglycemic effect of Panax notoginseng polysaccharide (PNS) on diabetic rats and the therapeutic effect of streptozotocin (STZ) on diabetic ophthalmopathy in rats, and to elucidate its mechanism. Methods 70 Sprague-Dawley male rats were divided into two groups: control group (n = 10) and model group (n = 60). Rats in the model group were fed with high-fat diet for .2 weeks, and rats in the model group were given intraperitoneal injection of 35mg kg-1 STZ to make hyperglycemic model for 3 days. Fasting for 12 h, fasting blood glucose (FBG) level was measured. FBG11.1mmol L-1 was used as the successful standard for modeling. The rats were randomly divided into two groups: model group, metformin (150mg kg-1) group, low, medium and high dose (75150 and 300mg kg-1) panax notoginseng polysaccharide group. After 5 and 8 weeks of intragastric administration, the levels of FBG in rats were measured. After 8 weeks of administration, the glucose tolerance of rats was measured. The liver of the rats was sacrificed to detect the levels of liver glycogen, and the levels of serum glutathione (GSH) and nitric oxide (NO) were detected. The expression of vascular endothelial growth factor (VEGF) and inducible nitric oxide synthase (iNOS) were detected by Q-PCR in isolated rat retina. Results: compared with the model group, the level of FBG, glucose tolerance and liver glycogen in the middle dose panax notoginseng polysaccharide group decreased significantly after 5 weeks (P0.05), and after 8 weeks, the glucose tolerance and liver glycogen level decreased significantly (P0.05 or P0.01). Compared with the model group, the serum level of GSH in the high dose panax notoginseng polysaccharide group was significantly increased and decreased (P0.05 or P0.01) .Q-PCR, and compared with the model group. The expression of VEGF and iNOS in the retina of high dose panax notoginseng polysaccharide group was significantly decreased (P0.05) .HE staining, compared with the model group, moderate and low doses of panax notoginseng polysaccharide group rats retinal vascular hyperplasia, no retinal atrophy; The retinal vascular hyperplasia of rats in high dose panax notoginseng polysaccharide group was obviously improved, and no retinal atrophy was found. Conclusion: Panax notoginseng polysaccharides can decrease blood glucose and increase the expression of VEGF and iNOS genes by increasing the levels of GSH and no, and can play a role in the treatment of diabetic retinopathy.
【作者單位】： 承德醫(yī)學院附屬醫(yī)院眼科;
【基金】：河北省科技廳自然科學基金資助課題(H2015406054)
【分類號】：R285.5;R-332

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