三七多糖對糖尿病模型大鼠的降血糖作用和眼視網(wǎng)膜病變的治療作用及其機制
[Abstract]:Aim: to investigate the hypoglycemic effect of Panax notoginseng polysaccharide (PNS) on diabetic rats and the therapeutic effect of streptozotocin (STZ) on diabetic ophthalmopathy in rats, and to elucidate its mechanism. Methods 70 Sprague-Dawley male rats were divided into two groups: control group (n = 10) and model group (n = 60). Rats in the model group were fed with high-fat diet for .2 weeks, and rats in the model group were given intraperitoneal injection of 35mg kg-1 STZ to make hyperglycemic model for 3 days. Fasting for 12 h, fasting blood glucose (FBG) level was measured. FBG11.1mmol L-1 was used as the successful standard for modeling. The rats were randomly divided into two groups: model group, metformin (150mg kg-1) group, low, medium and high dose (75150 and 300mg kg-1) panax notoginseng polysaccharide group. After 5 and 8 weeks of intragastric administration, the levels of FBG in rats were measured. After 8 weeks of administration, the glucose tolerance of rats was measured. The liver of the rats was sacrificed to detect the levels of liver glycogen, and the levels of serum glutathione (GSH) and nitric oxide (NO) were detected. The expression of vascular endothelial growth factor (VEGF) and inducible nitric oxide synthase (iNOS) were detected by Q-PCR in isolated rat retina. Results: compared with the model group, the level of FBG, glucose tolerance and liver glycogen in the middle dose panax notoginseng polysaccharide group decreased significantly after 5 weeks (P0.05), and after 8 weeks, the glucose tolerance and liver glycogen level decreased significantly (P0.05 or P0.01). Compared with the model group, the serum level of GSH in the high dose panax notoginseng polysaccharide group was significantly increased and decreased (P0.05 or P0.01) .Q-PCR, and compared with the model group. The expression of VEGF and iNOS in the retina of high dose panax notoginseng polysaccharide group was significantly decreased (P0.05) .HE staining, compared with the model group, moderate and low doses of panax notoginseng polysaccharide group rats retinal vascular hyperplasia, no retinal atrophy; The retinal vascular hyperplasia of rats in high dose panax notoginseng polysaccharide group was obviously improved, and no retinal atrophy was found. Conclusion: Panax notoginseng polysaccharides can decrease blood glucose and increase the expression of VEGF and iNOS genes by increasing the levels of GSH and no, and can play a role in the treatment of diabetic retinopathy.
【作者單位】： 承德醫(yī)學(xué)院附屬醫(yī)院眼科;
【基金】：河北省科技廳自然科學(xué)基金資助課題(H2015406054)
【分類號】：R285.5;R-332
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