黑猩猩和人類左腦成纖維細(xì)胞生長(zhǎng)因子1（FGF1）不同分子網(wǎng)絡(luò)和機(jī)制的功能預(yù)測(cè)
發(fā)布時(shí)間：2018-07-31 19:24
【摘要】：在本文中,我們的目標(biāo)是分別建立黑猩猩左腦和人類左腦的上下游激活和抑制網(wǎng)絡(luò)。我們使用的芯片中,包含了12558個(gè)來自于15個(gè)黑猩猩左腦樣本和14個(gè)人類左腦樣本的基因。我們所使用的顯著性表達(dá)基因標(biāo)記,是通過使用微陣列的顯著性差異分析方法而得到的。我們通過將數(shù)據(jù)進(jìn)行l(wèi)og2的歸一化處理,并運(yùn)用聚類分析方法進(jìn)行分析。首先,我們使用GRNInfer工具,構(gòu)建了441個(gè)具有顯著性高和低表達(dá)分子的整體網(wǎng)絡(luò)；第二,我們從已構(gòu)建的黑猩猩和人類左腦的整體網(wǎng)絡(luò)中,確定了成纖維細(xì)胞生長(zhǎng)因子1 (FGF1)的表達(dá)特性；第三,我們確定了黑猩猩和人類左腦的成纖維細(xì)胞生長(zhǎng)因子1 (FGF1)的上下游網(wǎng)絡(luò)；第四,我們從黑猩猩和人類左腦的成纖維細(xì)胞生長(zhǎng)因子1 (FGF1)的上下游網(wǎng)絡(luò)中進(jìn)一步確認(rèn)了分子的激活和抑制特性。我們使用的生物學(xué)分析方法已經(jīng)得到了生物醫(yī)學(xué)領(lǐng)域相關(guān)專家的承認(rèn),并給予了高度評(píng)價(jià),所發(fā)表的論文分別刊登在SCI檢索的國(guó)際雜志上,例如Cell Biochemistry and Biophysics (影響因子4.311), Journal of Cellular Biochemistry(影響因子3.121), Cell Proliferation (影響因子2.742),Cellular and Molecular Neurobiology (影響因子2.423)。 在本文中,我們通過GO數(shù)據(jù)庫(kù)中的基因數(shù)據(jù)分析發(fā)現(xiàn),分子FGF1的相關(guān)模塊包括：細(xì)胞外區(qū),細(xì)胞外基質(zhì),細(xì)胞外空間,蛋白結(jié)合,生長(zhǎng)因子活性,肝素結(jié)合,血管生成,器官誘導(dǎo),信號(hào)傳導(dǎo),發(fā)育,細(xì)胞增殖,成細(xì)胞生長(zhǎng)因子受體信號(hào)通路,形態(tài)發(fā)生,細(xì)胞分化,肺發(fā)育,上皮細(xì)胞增殖的正調(diào)控。我們通過將黑猩猩的左腦和人類左腦進(jìn)行比較,從而在黑猩猩的左腦中分別構(gòu)建了低表達(dá)的成纖維細(xì)胞生長(zhǎng)因子1 (FGF1)的DNA損傷介導(dǎo)的雙鏈斷裂重組修復(fù),耦合了細(xì)胞分裂到端粒維持的抗凋亡網(wǎng)絡(luò)和成纖維細(xì)胞生長(zhǎng)因子1 (FGF1)的先天免疫驅(qū)動(dòng)的轉(zhuǎn)錄耦合端粒維持的縮短導(dǎo)致細(xì)胞凋亡的調(diào)控網(wǎng)絡(luò)。而成纖維細(xì)胞生長(zhǎng)因子1 (FGF1)在人類左腦中,當(dāng)基因表達(dá)改變最小倍數(shù)設(shè)為2倍時(shí)表現(xiàn)為高表達(dá)的特性。我們?cè)谌祟愖竽X中分別構(gòu)建了高表達(dá)的成纖維細(xì)胞生長(zhǎng)因子1 (FGF1)的防御響應(yīng)介導(dǎo)的遷移耦合綁定運(yùn)輸?shù)男盘?hào)到氧化代謝的誘導(dǎo)樹突發(fā)育網(wǎng)絡(luò)和高表達(dá)的成纖維細(xì)胞生長(zhǎng)因子1 (FGF1)的DNA修復(fù)的遷移耦合到軸突擴(kuò)建正調(diào)控轉(zhuǎn)錄抑制網(wǎng)絡(luò)。我們的研究結(jié)果表明,在黑猩猩左腦中,上游激活網(wǎng)絡(luò)：分子C10ORF10, CDC25B, LOH11CR2A, RAD50, SAPS2, STAMBP激活FGF1;而在下游的激活網(wǎng)絡(luò)中,FGF1沒有激活任何分子；上游抑制網(wǎng)絡(luò)：AL049278, AL080232, CFHR1, CTBP1, DDX3Y, RNF2, RPP14, SARM1, TERF1_1抑制FGF1;而下游抑制網(wǎng)絡(luò)沒有相應(yīng)的結(jié)果。在人類的左腦中,上游激活網(wǎng)絡(luò)：分子CTRL, GPD1, LGALS3BP, MAP1B_3, PCDHGA8, PCSK6, PDIA2激活FGF1;而在下游的激活網(wǎng)絡(luò)中,FGF1激活分子MGC15523, NUPR1, UBXD2;上游網(wǎng)絡(luò)：DTNA, FOXN3_1, MAPT, NAIP, NR1D2_2, SLC25A46, SMG1抑制FGF1;而下游網(wǎng)絡(luò)：FGF1抑制分子ISCA1, PSMA4, U79289。我們對(duì)成纖維細(xì)胞生長(zhǎng)因子1 (FGF1)的研究對(duì)于神經(jīng)退化性疾病的研究以及臨床治療具有重要作用,對(duì)于利用基因進(jìn)行疾病的病理性研究具有重要意義。
[Abstract]:In this article, our goal is to establish the upstream and downstream activation and suppression networks of the left brain of the chimpanzee and the human left brain. In the chips we use, we include 12558 genes from 15 chimpanzee left brain samples and 14 human left brain samples. The method of sex difference analysis is obtained. We analyze the data by normalizing the log2 and using clustering analysis. First, we use the GRNInfer tool to build 441 overall networks with significant and low expression molecules; second, we are from the built chimpanzee and the human left brain as a whole network, The expression characteristics of fibroblast growth factor 1 (FGF1) were determined; third, we identified the upstream and downstream networks of chimpanzee and human left brain fibroblast growth factor 1 (FGF1); fourth, we further confirmed the activation of the molecules from the upstream and downstream networks of the fibroblast growth factor 1 (FGF1) of the left brain of the human and the human left brain. The biological analysis methods we use have been recognized by relevant experts in the field of biomedicine and are highly evaluated. The published papers are published in the International Journal of SCI retrieval, such as Cell Biochemistry and Biophysics (influence factor 4.311), Journal of Cellular Biochemistry (influence factor 3) .121), Cell Proliferation (influence factor 2.742), Cellular and Molecular Neurobiology (influence factor 2.423).
In this paper, we found that the related modules of molecular FGF1 include extracellular domain, extracellular matrix, extracellular space, protein binding, growth factor activity, heparin binding, angiogenesis, organ induction, signal transduction, propagation, cell proliferation, cell growth factor receptor signaling pathway, morphologic development. The positive regulation of cell differentiation, lung development and epithelial cell proliferation. We have compared the left brain of chimpanzee with the human left brain, thus constructing a low expressed DNA damage mediated double strand break recombination repair in the left brain of the chimpanzee, which coupled cell division to telomere maintenance. Apoptotic network and fibroblast growth factor 1 (FGF1), the shortening of the congenital immune driven transcription coupling telomere maintenance, which leads to apoptosis, and fibroblast growth factor 1 (FGF1) in the human left brain, when the minimum multiplier of gene expression is set to 2 times as high expression. We divide the human left brain into the human left brain. Do not construct a highly expressed fibroblast growth factor 1 (FGF1) defense response mediated transport coupled binding transport signal to oxidative metabolism induced dendritic development network and high expression of fibroblast growth factor 1 (FGF1) DNA repair transfer coupling to the axon expansion positive regulatory transcriptional inhibition network. In the left brain of chimpanzee, the upstream activation network: molecules C10ORF10, CDC25B, LOH11CR2A, RAD50, SAPS2, STAMBP activate FGF1; and in the downstream activation network, FGF1 does not activate any molecules; the upstream suppression network: AL049278, AL080232, CFHR1, CTBP1, CTBP1, repressive, restraining networks; and downstream inhibition networks In the human left brain, the upstream activation network: molecular CTRL, GPD1, LGALS3BP, MAP1B_3, PCDHGA8, PCSK6, PDIA2 activate FGF1; and in the downstream activation network, FGF1 activator MGC15523, NUPR1, UBXD2; upstream network: downstream network: GF1 inhibitory molecules ISCA1, PSMA4, U79289., our study of fibroblast growth factor 1 (FGF1) has an important role in the research and clinical treatment of neurodegenerative diseases, and is of great significance for the use of gene for pathological study of disease.
【學(xué)位授予單位】：北京郵電大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R363
本文編號(hào)：2156589
[Abstract]:In this article, our goal is to establish the upstream and downstream activation and suppression networks of the left brain of the chimpanzee and the human left brain. In the chips we use, we include 12558 genes from 15 chimpanzee left brain samples and 14 human left brain samples. The method of sex difference analysis is obtained. We analyze the data by normalizing the log2 and using clustering analysis. First, we use the GRNInfer tool to build 441 overall networks with significant and low expression molecules; second, we are from the built chimpanzee and the human left brain as a whole network, The expression characteristics of fibroblast growth factor 1 (FGF1) were determined; third, we identified the upstream and downstream networks of chimpanzee and human left brain fibroblast growth factor 1 (FGF1); fourth, we further confirmed the activation of the molecules from the upstream and downstream networks of the fibroblast growth factor 1 (FGF1) of the left brain of the human and the human left brain. The biological analysis methods we use have been recognized by relevant experts in the field of biomedicine and are highly evaluated. The published papers are published in the International Journal of SCI retrieval, such as Cell Biochemistry and Biophysics (influence factor 4.311), Journal of Cellular Biochemistry (influence factor 3) .121), Cell Proliferation (influence factor 2.742), Cellular and Molecular Neurobiology (influence factor 2.423).
In this paper, we found that the related modules of molecular FGF1 include extracellular domain, extracellular matrix, extracellular space, protein binding, growth factor activity, heparin binding, angiogenesis, organ induction, signal transduction, propagation, cell proliferation, cell growth factor receptor signaling pathway, morphologic development. The positive regulation of cell differentiation, lung development and epithelial cell proliferation. We have compared the left brain of chimpanzee with the human left brain, thus constructing a low expressed DNA damage mediated double strand break recombination repair in the left brain of the chimpanzee, which coupled cell division to telomere maintenance. Apoptotic network and fibroblast growth factor 1 (FGF1), the shortening of the congenital immune driven transcription coupling telomere maintenance, which leads to apoptosis, and fibroblast growth factor 1 (FGF1) in the human left brain, when the minimum multiplier of gene expression is set to 2 times as high expression. We divide the human left brain into the human left brain. Do not construct a highly expressed fibroblast growth factor 1 (FGF1) defense response mediated transport coupled binding transport signal to oxidative metabolism induced dendritic development network and high expression of fibroblast growth factor 1 (FGF1) DNA repair transfer coupling to the axon expansion positive regulatory transcriptional inhibition network. In the left brain of chimpanzee, the upstream activation network: molecules C10ORF10, CDC25B, LOH11CR2A, RAD50, SAPS2, STAMBP activate FGF1; and in the downstream activation network, FGF1 does not activate any molecules; the upstream suppression network: AL049278, AL080232, CFHR1, CTBP1, CTBP1, repressive, restraining networks; and downstream inhibition networks In the human left brain, the upstream activation network: molecular CTRL, GPD1, LGALS3BP, MAP1B_3, PCDHGA8, PCSK6, PDIA2 activate FGF1; and in the downstream activation network, FGF1 activator MGC15523, NUPR1, UBXD2; upstream network: downstream network: GF1 inhibitory molecules ISCA1, PSMA4, U79289., our study of fibroblast growth factor 1 (FGF1) has an important role in the research and clinical treatment of neurodegenerative diseases, and is of great significance for the use of gene for pathological study of disease.
【學(xué)位授予單位】：北京郵電大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R363
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1 王雅丹;胡豫;張璐;孫春艷;;腦源性神經(jīng)營(yíng)養(yǎng)因子通過AKT和ERK1/2信號(hào)通路誘導(dǎo)內(nèi)皮細(xì)胞血管生成(英文)[J];中國(guó)實(shí)驗(yàn)血液學(xué)雜志;2008年01期
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