【摘要】：目的探討氣管和腹腔注射2種給藥方式復(fù)制特發(fā)性肺纖維化(idiopathic pulmonary fibrosis,IPF)大鼠模型的差異。方法240~260g雄性SD大鼠24只,按照隨機(jī)數(shù)字表法分為氣管對照組(QC組)、氣管給藥組(Q組)、腹腔對照組(FC組)和腹腔給藥組(F組),每組6只。分別經(jīng)氣管一次性注射生理鹽水0.2mL或博萊霉素(BLM)5mg·kg~(-1)·d~(-1);腹腔注射生理鹽水0.2mL或博萊霉素15mg·kg~(-1)·d~(-1),連續(xù)10d。28d后處死。觀察每組小鼠生存率、體質(zhì)量、肺臟外觀、病理改變及肺組織羥脯氨酸含量。結(jié)果 Q組生存率為66.7%;F組生存率為83.3%,二者比較差異有統(tǒng)計學(xué)意義(P0.05)。2種方法給藥組大鼠體質(zhì)量均減輕,F組動物體質(zhì)量在1~7d內(nèi)下降,第8天起開始恢復(fù);Q組動物體質(zhì)量在前15d內(nèi)持續(xù)下降,第16d起開始恢復(fù)。2種方法給藥組的肺臟外觀基本相同,沒有顯著差別。病理檢查證實,Q組的膠原纖維沉積主要分布在氣管周圍,而F組的膠原沉積則分布在胸膜和肺間質(zhì)。2種方法復(fù)制的模型組肺組織羥脯氨酸含量均顯著高于對照組,差異有統(tǒng)計學(xué)意義(P0.05);模型組間差異無統(tǒng)計學(xué)意義。結(jié)論氣管給藥與腹腔給藥對于復(fù)制大鼠IPF無明顯差別,但氣管給藥法更方便、經(jīng)濟(jì)和可靠。
[Abstract]:Objective to investigate the difference between trachea and intraperitoneal administration of (idiopathic pulmonary fibrosis (IPF) rat models of idiopathic pulmonary fibrosis (IPF). Methods 24 male SD rats were randomly divided into three groups: trachea control group (QC group), tracheal administration group (Q group), peritoneal control group (FC group) and intraperitoneal administration group (F group), with 6 rats in each group. Normal saline 0.2mL or bleomycin (BLM) 5mg kg ~ (-1) d ~ (-1) were injected through trachea and intraperitoneal injection of normal saline 0.2mL or bleomycin 15mg (15mg ~ (-1) d ~ (-1) were performed after 10d.28d. Survival rate, body mass, lung appearance, pathological changes and hydroxyproline content were observed in each group. Results the survival rate of group Q was 66.7% and the survival rate of group F was 83.3%. The difference between the two groups was statistically significant (P0.05). On the 8th day, the mass of animals and animals in group Q decreased continuously in the first 15 days, and the lung appearance of group 2 was the same as that of the control group on the 16th day, and there was no significant difference between the two groups. Pathological examination showed that collagen fiber deposition in group Q was mainly distributed around trachea, while collagen deposition in group F was significantly higher than that in control group (P < 0.01), but the content of hydroxyproline in lung tissue of group F was significantly higher than that of control group (P < 0.01), while that of group F was significantly higher than that of control group (P < 0.05). The difference was statistically significant (P0.05); there was no significant difference between the model groups. Conclusion there is no significant difference between tracheal administration and intraperitoneal administration in replicating IPF in rats, but tracheal administration is more convenient, economical and reliable.
【作者單位】： 第四軍醫(yī)大學(xué)藥學(xué)院天然藥物學(xué)教研室;第四軍醫(yī)大學(xué)學(xué)員旅;
【分類號】：R563;R-332

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