【摘要】：目的研究應(yīng)激大鼠腸道樹突細(xì)胞(DC)表型及功能狀態(tài)的改變。方法選用20只雄性SD大鼠隨機(jī)分為對(duì)照組、實(shí)驗(yàn)組,各10只。給予實(shí)驗(yàn)組大鼠結(jié)直腸擴(kuò)張聯(lián)合束縛刺激,對(duì)照組大鼠不處理,采用大鼠腹部收縮反射(AWR)對(duì)兩組大鼠進(jìn)行直腸氣囊擴(kuò)張,測(cè)量腸道容量感覺閾值。成功建立模型后,磁珠分選技術(shù)分離腸系膜淋巴結(jié)DC,檢測(cè)其表面CD80、CD86、MHCⅠ、MHCⅡ、Toll樣受體(TLR)4的表達(dá),將MLNDC與脾臟單個(gè)核細(xì)胞體外混合培養(yǎng),流式標(biāo)記CD4+/CD8+T淋巴細(xì)胞,檢測(cè)MLNDC促CD4+/CD8+T淋巴細(xì)胞增殖的能力。結(jié)果實(shí)驗(yàn)組大鼠的疼痛閾值明顯低于對(duì)照組(P0.05);實(shí)驗(yàn)組大鼠MLNDC表達(dá)MHCⅡ水平高于對(duì)照組(P0.05),而表達(dá)CD80、CD86、MHCI與對(duì)照組比較差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);實(shí)驗(yàn)組大鼠MLNDC表達(dá)TLR4高于對(duì)照組(P0.05);與對(duì)照組相比,實(shí)驗(yàn)組大鼠MLNDC促CD4+/CD8+T淋巴細(xì)胞增殖能力增強(qiáng)(P0.05)。結(jié)論大鼠應(yīng)激刺激后腸道DC上調(diào)表達(dá)TLR4,刺激其本身分化成熟,進(jìn)一步表達(dá)MHCⅡ,后者通過介導(dǎo)T淋巴細(xì)胞活化,引起腸道低度炎癥,從而引起腸道內(nèi)臟高敏感的產(chǎn)生。
[Abstract]:Objective to study the changes of (DC) phenotype and function of intestinal dendritic cells in stressed rats. Methods Twenty male SD rats were randomly divided into control group (n = 10) and experimental group (n = 10). Rats in the experimental group were given colorectal dilatation combined with bound stimulation, while the control group rats were not treated. The rats in the two groups were dilated by abdominal contractile reflex (AWR), and the sensory threshold of intestinal volume was measured. After successfully establishing the model, the mesenteric lymph nodes were isolated by magnetic bead sorting technique, and the expression of CD86MHC 鈪，

本文編號(hào)：2148887