【摘要】：目的從子鼠海馬中細(xì)胞骨架蛋白Arc的變化,來探討豐富環(huán)境方式對孕期慢性應(yīng)激所產(chǎn)子鼠學(xué)習(xí)能力影響的干預(yù)機(jī)制。方法建立孕期慢性應(yīng)激大鼠模型,并采用豐富環(huán)境干預(yù)子鼠,利用水迷宮和Y-迷宮測定子鼠學(xué)習(xí)記憶能力、RT-PCR和Western Blotting測定海馬中Arc表達(dá)情況。結(jié)果模型組母鼠皮質(zhì)酮增高,提示模型組母鼠處于應(yīng)激狀態(tài)。豐富環(huán)境后,子鼠血漿皮質(zhì)酮降低(P0.05);水迷宮中逃避潛伏期和跨平臺次數(shù)、Y-迷宮中所需的訓(xùn)練次數(shù)和正確反應(yīng)率情況均轉(zhuǎn)好(P均0.05);經(jīng)豐富環(huán)境后,模型子鼠組海馬組織Arc mRNA和蛋白表達(dá)增高(P均0.05)。結(jié)論豐富環(huán)境可以作為孕期受到應(yīng)激因素影響學(xué)習(xí)能力提高的手段,與血漿皮質(zhì)酮降低及海馬突觸相關(guān)蛋白Arc升高有關(guān)。
[Abstract]:Objective to investigate the effect of rich environment on learning ability of rats born under chronic stress from the changes of cytoskeleton Arc in hippocampus. Methods the rat model of chronic stress during pregnancy was established, and the rich environment was used to interfere with the rats. The learning and memory abilities of the rats were measured by water maze and Ymaze. RT-PCR and Western Blotting were used to detect the expression of Arc in hippocampus. Results the level of corticosterone in the model group was higher than that in the control group, indicating that the model group was in stress state. After the rich environment, the plasma corticosterone decreased (P0.05), the training times and correct reaction rate of escape latency and cross-platform times in the water maze were improved (P 0.05), and after the rich environment, the training times and the correct reaction rate of Y- labyrinth were improved (P 0.05). The expression of Arc mRNA and protein in hippocampus of the model group was higher than that of the control group (P < 0. 05). Conclusion the rich environment can be used as a means to improve learning ability due to stress factors during pregnancy, which is related to the decrease of plasma corticosterone and the increase of hippocampal synaptophysin Arc.
【作者單位】： 新疆醫(yī)科大學(xué)公共衛(wèi)生學(xué)院社會醫(yī)學(xué)教研室;新疆醫(yī)科大學(xué)公共衛(wèi)生學(xué)院勞動衛(wèi)生與環(huán)境衛(wèi)生學(xué)教研室;
【基金】：新疆維吾爾自治區(qū)青年科學(xué)基金項目(編號:2013211B50)
【分類號】：R338
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本文編號：2141958