【摘要】：目的探討不同劑量酵母、腺嘌呤和不同造模持續(xù)時間對大鼠痛風性腎病模型的影響。方法模型組雄性SD大鼠用15 g/kg酵母粉和50 mg/kg、100 mg/kg腺嘌呤分別灌胃,同時分別設置8、10、22、48的造模天數(shù)。比較各組腎臟肥大指數(shù)的大小,檢測大鼠腎功能,尿液指標的變化,腎皮質(zhì)β-N-乙酰氨基葡萄糖苷酶(NAG)含量,肝臟黃嘌呤氧化酶(XOD)活性的變化,同時觀察大鼠腎臟病理改變。并用免疫組化技術(shù)檢測腎臟組織NGAL、OAT1和TIMP-1的表達。結(jié)果隨著模型組造模劑濃度的增加和造模時間的延長,腎臟病理改變越明顯。100mg/kg腺嘌呤和15g/kg酵母共同造模8 d,與正常對照組比較,該組腎臟病理組織變化明顯,炎性細胞浸潤腎小管上皮細胞腫脹,腎小管和腎小球結(jié)構(gòu)未被破壞;腎臟肥大指數(shù)較正常對照組增大(P0.01);與正常對照組比較,模型A組,B組、E組、F組腎功能下降(P0.01),模型A組、B組、E組、F組大鼠的24 h尿量,尿蛋白含量增多(P0.01)。模型E組大鼠腎組織NGAL、TIMP-1的表達高于正常組大鼠(P0.01),OAT1的表達低于正常組大鼠(P0.01)。結(jié)論 100 mg/kg腺嘌呤和15 g/kg酵母共同造模8 d為較理想的造模劑量和造模持續(xù)時間,痛風性腎病模型大鼠腎損傷受蛋白NGAL、TIMP-1和OAT1的影響。
[Abstract]:Objective to investigate the effects of different doses of yeast, adenine and duration on gouty nephropathy in rats. Methods male Sprague-Dawley rats in the model group were fed with 15 g/kg yeast powder and 50 mg / kg ~ 100 mg/kg adenine respectively. The changes of renal function, urine index, 尾 -N-acetylglucosaminidase (nag) content and xanthine oxidase (XOD) activity in renal cortex were measured. The expression of NGALA OAT1 and TIMP-1 in renal tissue was detected by immunohistochemistry. Results with the increase of the concentration of the model agent and the prolongation of the time of model making, the pathological changes of kidney were more obvious. 100 mg / kg adenine and 15g/kg yeast were used to model the kidney for 8 days. Compared with the normal control group, the pathological changes of kidney in this group were obvious. Inflammatory cells infiltrated renal tubular epithelial cells, tubule and glomerular structure were not destroyed, renal hypertrophy index was higher than normal control group (P0.01), compared with normal control group, The renal function of model group A and B was decreased (P0.01), and the 24 h urine volume and urine protein content were increased (P0.01). The expression of NGALP TIMP-1 in renal tissue of model group E was higher than that of normal group (P0.01) and the expression of OAT1 was lower than that of normal group (P0.01). Conclusion the optimal dose and duration of model making were 100 mg/kg adenine and 15 g/kg yeast for 8 days. The renal injury of rats with gouty nephropathy was affected by the protein NGALL TIMP-1 and OAT1.
【作者單位】： 廣州中醫(yī)藥大學中藥學院中藥分析實驗室;
【基金】：南藥研究協(xié)同創(chuàng)新中心專項基金(2011)
【分類號】：R589.7;R692;R-332

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