【摘要】：目的探討蜘蛛香環(huán)烯醚萜類成分纈草醛對慢-急性應(yīng)激致腸易激綜合征(IBS)模型大鼠的治療作用及其對5-羥吲哚乙酸(5-HIAA)及5-羥色胺(5-HT)相關(guān)受體蛋白表達的影響作用。方法將SD雄性大鼠平均隨機分為空白對照組、模型組及纈草醛高、中、低劑量組(0.6、0.3、0.15 mg/kg)、匹維溴銨組(25 mg/kg)。采用慢-急性應(yīng)激結(jié)合孤養(yǎng)的方法建立IBS大鼠模型,觀察纈草醛對IBS模型大鼠腹部回撤反射(AWR)評分的影響作用;使用高效液相色譜法測定大鼠結(jié)腸中5-HIAA含量;使用Western Blot法檢測纈草醛對IBS模型大鼠結(jié)腸5-HT1A、5-HT3A及5-HTR4受體蛋白表達的影響。結(jié)果與模型組相比,纈草醛各劑量組均能夠降低IBS模型大鼠AWR評分,并降低結(jié)腸內(nèi)5-HIAA含量(P0.05);纈草醛各劑量組均可調(diào)節(jié)結(jié)腸內(nèi)5-HT1A、5-HT3A及5-HTR4受體的蛋白表達。結(jié)論纈草醛對慢-急性應(yīng)激致IBS模型大鼠的胃腸道功能亢進有明顯抑制作用,可降低內(nèi)臟敏感性,提示蜘蛛香環(huán)烯醚萜對IBS的癥狀有改善作用,作用機制可能與調(diào)節(jié)結(jié)腸內(nèi)5-HIAA含量及5-HT相關(guān)受體的蛋白表達水平相關(guān)。
[Abstract]:Objective to investigate the therapeutic effect of valeraldehyde, a terpenoid component of arachnidism cyclene ether, on the expression of 5-hydroxyindoleacetic acid (5-HIAA) and 5-hydroxytryptamine (5-HT) receptor protein in rats with irritable bowel syndrome (IBS) induced by slow and acute stress. Methods Sprague-Dawley male rats were randomly divided into blank control group, model group, high, middle and low doses of valerinaldehyde (0.6? 0. 3? 0. 15 mg/kg) and pividimine group (25? mg/kg). The effects of valeraldehyde on abdominal withdrawal reflex (awr) score of IBS rats were observed by slow acute stress combined with solitary nourishment, and the content of 5-HIAA in colon of rats was determined by high performance liquid chromatography (HPLC). The effects of valeraldehyde on the expression of 5-HT1A1-5-HT3A and 5-HTR4 receptor protein in colon of rats with IBS were detected by Western blot. Results compared with the model group, valeraldehyde could decrease the AWR score and the content of 5-HIAA in the colon of the IBS model rats (P0.05), and the protein expression of 5-HT1A1-5-HT3A and 5-HTR4 receptor in the colon could be regulated by valeraldehyde in each dose group. Conclusion Valerianaldehyde has obvious inhibitory effect on gastrointestinal hyperfunction and visceral sensitivity in IBS model rats induced by slow and acute stress. It suggests that aracyclene ether terpenes can improve the symptoms of IBS. The mechanism may be related to the regulation of 5-HIAA content and the protein expression of 5-HT-related receptors in colon.
【作者單位】： 北京中醫(yī)藥大學(xué)中醫(yī)學(xué)院;北京中醫(yī)藥大學(xué)中藥學(xué)院;
【基金】：國家自然科學(xué)基金資助項目(No.81374044) 北京中醫(yī)藥大學(xué)校級自主課題資助項目(No.2016-JYB-XS028)~~
【分類號】：R285.5;R-332

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