本文選題：ghrelin + 隔核�。� 參考：《青島大學(xué)》2012年碩士論文

【摘要】：目的：探討外側(cè)隔核ghrelin對大鼠胃運動的調(diào)控及機制。 方法：采用核團微量注射、傳感器胃內(nèi)置入及免疫組化研究方法,記錄清醒自由活動大鼠胃運動幅度和頻率的變化,探討外側(cè)隔核(lateral septal nucleus, LS)微量注射ghrelin及其受體阻斷劑(D-Lys3-GHRP-6)對大鼠胃運動的影響,并觀察迷走復(fù)合體(DVC)內(nèi)c-Fos的表達,及雙側(cè)膈下迷走神經(jīng)切斷后,LS注射ghrelin對大鼠胃運動的影響。 結(jié)果：①外側(cè)隔核分別注射20 nmol,200 nmol或2000 nmol ghrelin 6min后,大鼠胃收縮幅度顯著增高(P0.05-0.01),胃收縮頻率顯著加快(P0.05-0.01),且呈顯著劑量依賴關(guān)系。外側(cè)隔核注入ghrelin+D-Lys3-GHRP-6混合液6 min后,與ghrelin+NS組比較,ghrelin促進胃收縮幅度(P0.05-0.01)和頻率(P0.05)的作用消失,但單用ghrelin受體拮抗劑D-Lys3-GHRP-6對胃收縮幅度(P0.05)和收縮頻率(P0.05)均無顯著影響；②外側(cè)隔核注射200nmol ghrelin 90 min后,與NS組比較,大鼠迷走復(fù)合體內(nèi)c-Fos表達明顯增加(P0.01)；③膈下迷走神經(jīng)切斷后,大鼠胃收縮幅度(P0.05)和收縮頻率(P0.05)顯著降低；在膈下迷走神經(jīng)切斷大鼠,與假手術(shù)對照組相比,外側(cè)隔核注射200nmol ghrelin,胃收縮幅度(P0.05-0.01)和收縮頻率(P0.05)顯著降低,但與迷走神經(jīng)切斷+NS組相比,胃收縮幅度(P0.05)和頻率(P0.05)無顯著改變。 結(jié)論：Ghrelin在外側(cè)隔核通過作用其受體顯著促進大鼠胃運動。Ghrelin在外側(cè)隔核促進胃運動的作用可能下行通過迷走復(fù)合體-迷走神經(jīng)通路實現(xiàn)。
[Abstract]:Objective: to investigate the regulation and mechanism of ghrelin in lateral septal nucleus on gastric motility in rats. Methods: the changes of the amplitude and frequency of gastric motility were recorded by microinjection of nucleus, intragastric implantation of sensors and immunohistochemical study in rats with conscious and free movement. To investigate the effect of microinjection of ghrelin and its receptor blocker (D-Lys3-GHRP-6) into the lateral septal nucleus (lateral septal nucleus, LS) on gastric motility in rats, and to observe the expression of c-Fos in vagus complex and the effect of ghrelin injection on gastric motility after bilateral subphrenic vagotomy. Results the gastric contraction amplitude and gastric contraction frequency were significantly increased (P0.05-0.01) and gastric contraction frequency (P0.05-0.01) were increased in a dose-dependent manner after injection of 20 nmol or 2000 nmol ghrelin 6min into the lateral septal nucleus. After injection of ghrelin D-Lys3-GHRP-6 for 6 min, the effects of ghrelin on gastric contraction amplitude (P0.05-0.01) and frequency (P0.05) were disappeared compared with those in ghrelin NS group, but D-Lys3-GHRP-6 alone had no significant effect on gastric contractile amplitude (P0.05) and contractile frequency (P0.05). 2After the injection of 200nmol ghrelin 90 min into the lateral septal nucleus, compared with the NS group, the expression of c-Fos increased significantly (P0.01) after subphrenic vagotomy, the gastric contraction amplitude (P0.05) and the contractile frequency (P0.05) were significantly decreased. After subphrenic vagotomy, the amplitude of gastric contraction (P0.05-0.01) and the frequency of gastric contraction (P0.05) were significantly decreased after injection of 200nmol ghrelin in the lateral septal nucleus compared with the sham operated control group, but the amplitude of gastric contraction (P0.05) and the frequency of gastric contraction (P0.05) were not significantly changed compared with the NS group. Conclusion the effects of the receptor in the lateral septal nucleus on the gastric motility of rat. Ghrelin in the lateral septal nucleus may be mediated by vagus pathway via vagus pathway.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R338

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