本文選題：白細(xì)胞介素-1β + 瘦素　； 參考：《青島大學(xué)》2011年碩士論文

【摘要】：目的通過研究不同濃度的IL-1β(Interleukin-1β, IL-1β)和瘦素(leptin)對子宮內(nèi)膜細(xì)胞所分泌的與胚胎著床密切相關(guān)的細(xì)胞間粘附分子-1 (Intercellular Adhesion Mo-lecule-1, ICAM-1)和基質(zhì)金屬蛋白酶9(Matrix Metalloproteinase 9, MMP9)表達(dá)的影響,來探討IL-1β和leptin對胚胎著床的影響,為提高胚胎種植率提供理論依據(jù)。方法取育齡婦女分泌中期的子宮內(nèi)膜進(jìn)行體外培養(yǎng),分別用不同濃度的IL-1β、leptin干預(yù),采用免疫細(xì)胞化學(xué)方法明確ICAM-1和MMP9在子宮內(nèi)膜腺體細(xì)胞或基質(zhì)細(xì)胞的定位表達(dá)；采用ELISA方法測定干預(yù)后培養(yǎng)液中ICAM-1和MMP9的表達(dá),并用半定量RT-PCR方法檢測子宮內(nèi)膜細(xì)胞中IC AM-1 mRNA和MMP9mRNA的表達(dá)。 結(jié)果(1)免疫細(xì)胞化學(xué)：所培養(yǎng)的細(xì)胞為子宮內(nèi)膜來源的細(xì)胞(包括腺體細(xì)胞和基質(zhì)細(xì)胞),并且發(fā)現(xiàn)不論干預(yù)前后,ICAM-1主要在腺體細(xì)胞中表達(dá),而基質(zhì)細(xì)胞只有很少的表達(dá),而MMP9在基質(zhì)和腺體細(xì)胞中均有表達(dá)；(2) ELISA:與對照組相比,ICAM-1經(jīng)lOng/ml的IL-1β干預(yù)后表達(dá)上調(diào)(P0.05),而分別經(jīng)0.1ng/ml、1ng/ml的IL-1β干預(yù)后表達(dá)無顯著性差異(P0.05)；MMP9分別經(jīng)1ng/ml、lOng/ml的IL-1β干預(yù)后表達(dá)上調(diào)(P0.05),而經(jīng)0.1ng/ml的IL-1β干預(yù)后表達(dá)無顯著性差異(P0.05)。ICAM-1經(jīng)100ng/ml的leptin干預(yù)后表達(dá)上調(diào)(P0.05),而經(jīng)lng/ml、lOng/ml的leptin干預(yù)后表達(dá)無顯著性差異(P0.05);MMP9分別經(jīng)1ng/ml、10ng/ml、100ng/ml的leptin干預(yù)后表達(dá)均上調(diào)(P0.05)。(3) RT-PCR:0.1ng/ml IL-1β干預(yù)后ICAM-1 mRNA的表達(dá)量與對照組無差異(P0.05), 1ng/ml IL-1β、10ng/ml IL-1β干預(yù)后,與對照組相比,ICAM-1 mRNA的表達(dá)增加,且隨濃度的升高,表達(dá)量呈上升趨勢(P0.05),分別經(jīng)不同濃度的IL-1β(0.1ng/ml、1ng/ml、10ng/ml), MMP9 mRNA較對照組相比表達(dá)均增加,且隨濃度的升高,表達(dá)量呈上升趨勢(P0.05)。而經(jīng)不同濃度的leptin(1ng/ml、10ng/ml、100ng/ml)干預(yù)后,ICAM-1、MMP9 mRNA較對照組相比表達(dá)均增加,且隨濃度的升高,表達(dá)量呈上升趨勢(P0.05)。 結(jié)論一定量的IL-1β和leptin可以增加體外培養(yǎng)分泌中期子宮內(nèi)膜細(xì)胞ICAM-1和MMP9表達(dá),且有劑量依賴性。
[Abstract]:Objective to investigate the effects of different concentrations of IL-1 尾 and leptin (leptin) on the expression of intercellular Adhesion Molecule-1 (ICAM-1) and matrix metalloproteinase 9 (MMP9) secreted by endometrial cells. To explore the effect of IL-1 尾 and leptin on embryo implantation and provide theoretical basis for increasing embryo implantation rate. Methods the endometrium of women of childbearing age was cultured in vitro, and the expression of ICAM-1 and MMP9 in endometrial glandular cells or stromal cells was determined by immunocytochemistry with different concentrations of IL-1 尾 -leptin. The expression of ICAM-1 and MMP9 was detected by Elisa and the expression of ICAM-1 mRNA and MMP9 mRNA in endometrial cells was detected by semi-quantitative RT-PCR. Results (1) Immunocytochemistry: the cultured cells were endometrial cells (including glandular cells and stromal cells), and it was found that ICAM-1 was mainly expressed in glandular cells before and after intervention, but only a little in stromal cells. The expression of MMP9 was up-regulated in stromal and glandular cells (P0.05) compared with control group (P0.05), but there was no significant difference after 0.1 ng / ml IL-1 尾 (P0.05). The expression of MMP9 was upregulated after 1 ng / ml of IL-1 尾 (P0.05), but there was no significant difference after the intervention of IL-1 尾 of 0.1ng/ml (P0.05). ICAM-1 was up-regulated by leptin of 100ng/ml (P0.05), but there was no significant difference in the expression of MMP9 after the intervention of leptin (P0.05). The expression of ICAM-1 mRNA was up-regulated (P0.05). (3). The expression of ICAM-1 mRNA was not different between the control group and the control group (P0.05) after the intervention of RT-PCR: 0.1ng / ml IL-1 尾 (P0.05). The expression of ICAM-1 mRNA was not different after the intervention of 1ng/ml IL-1 尾 10 ng / ml IL-1 尾. Compared with the control group, the expression of ICAM-1 mRNA increased, and the expression of ICAM-1 mRNA increased with the increase of the concentration (P0.05). After different concentrations of IL-1 尾 (0.1 ng / ml 1 ng / ml), the expression of MMP9 mRNA increased compared with the control group, and the expression of MMP9 mRNA increased with the increase of the concentration (P0.05). The expression of ICAM-1mMP9 was increased after different concentrations of leptin (1ng / ml 10ng / ml) compared with the control group, and the expression increased with the increase of the concentration (P0.05). Conclusion IL-1 尾 and leptin can increase the expression of ICAM-1 and MMP9 in endometrial cells in vitro in a dose-dependent manner.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R321

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