本文選題：EV71感染 + 模式識別受體��； 參考：《遵義醫(yī)學院》2012年碩士論文

【摘要】：目的：探討EV71感染患兒免疫活性細胞模式識別受體(Pattern recognition receptor, PRR)及細胞因子水平的變化。 方法：EV71感染患兒71例,其中輕癥EV71感染組20例,重癥EV71感染組25例,危重癥EV71感染組26例,同期同年齡正常對照組20例。采用Real-time PCR檢測外周血單個核細胞維甲酸蛋白(Retinoic acidinducible gene I, RIG-I)、黑色素瘤分化相關基因5(Melanoma differentitation-associated gene5,MDA5)、細胞因了(IL-12、IFN-a)表達；用流式細胞術檢測外周血單核／巨噬細胞(monocyte/macrophages, MC)、髓樣樹突狀細胞(myloid dentritic cells, mDC)及漿細胞樣樹突狀細胞(plasmacytoid dentritic cells, pDC) Toll樣受體(TLRs)表達率；用ELISA檢測細胞因子IL-12及IFN-a的變化。 結果：(1)輕癥EV71患兒僅TLR7升高,重癥EV71感染患兒外周血MC、 mDC、 pDC TLR7表達明顯升高(P0.05),MC、mDC高表達TLR2、3、4,危重癥患兒呈下降趨勢。(2)EV71感染患兒胞內(nèi)模式識別受體RIG-I/MDA5mRNA表達明顯增加；(3)輕癥患兒DC相關細胞因子有上升趨勢,重癥患兒DC相關細胞因子IL-12、 IFN-α明顯增高(P0.05),輕癥及危重癥患兒明顯降低(P0.05)。 結論：TLR7可能是免疫活性細胞識別EV71的主要模式識別受體；RIG-I/MDA5也可能參與識別EV71；合并細菌感染或細胞破壞釋放的內(nèi)源性配體導致TLR2或TLR4活化,誘導炎癥反應；IL-12、 IFN-α等細胞因子在重癥患兒明顯升高,危重癥患兒其細胞因子濃度明顯下降。
[Abstract]:Aim: to investigate the changes of pattern recognition receptor (PRR) and cytokine levels in children with EV71 infection. Methods 71 children with EV71 infection were divided into mild EV71 infection group (n = 20), severe EV71 infection group (n = 25), critical EV71 infection group (n = 26) and normal control group (n = 20). Retinoic acidinducible gene I (RIG-I) and Melanoma differentitation-associated gene 5 (MDA5) were detected by Real-time PCR. The expression rates of Toll-like receptors (TLRs) in peripheral blood mononuclear / macrophages (MC), myeloid dendritic cells (myloid dentritic cells, MDC) and plasma cell-like dendritic cells (plasmacytoid dentritic cells, PDC) were detected by flow cytometry, and cytokines IL-12 and IFN-a were detected by Elisa. Results: (1) only TLR7 increased in mild EV71 patients, while the expression of MCMC, mDCand pDC TLR7 in peripheral blood of patients with severe EV71 infection increased significantly (P0.05), and the expression of TLR2mDC was decreased in critically ill children. (2) the expression of RIG-Ir / MDA5 mRNA increased significantly in EV71 infected children. (3) DC related cytokines increased in mild children, IL-12 and IFN- 偽 increased significantly in severe children (P0.05), and decreased significantly in mild and critical children (P0.05). ConclusionTwo TLR7 may be the main pattern recognition receptor for EV71 recognition by immunoreactive cells, RIG-I / MDA5 may also be involved in EV71.Endogenous ligands associated with bacterial infection or cell destruction release may lead to the activation of TLR2 or TLR4 and induce inflammatory response. IL-12, IFN- 偽 and other cytokines increased significantly in severe children, and decreased significantly in critically ill children.
【學位授予單位】：遵義醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R392

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