發(fā)布時(shí)間：2018-07-03 15:11

  本文選題：綠萼梅總黃酮 + 慢性不可預(yù)見性溫和應(yīng)激��； 參考：《中國藥房》2017年13期

【摘要】：目的:考察綠萼梅總黃酮對慢性應(yīng)激抑郁模型大鼠抑郁行為的影響及機(jī)制。方法:將60只大鼠隨機(jī)分為生理鹽水組、模型組、氟西汀組(陽性對照,20 mg/kg)和綠萼梅總黃酮低、中、高劑量組(80、160、240 mg/kg),每組10只。除生理鹽水組外,其余各組大鼠均采用慢性不可預(yù)見性溫和應(yīng)激(CUMS)+孤養(yǎng)法復(fù)制抑郁模型,并于造模同時(shí)ig相應(yīng)藥物,每天1次,連續(xù)28 d。觀察大鼠體質(zhì)量、攝食量的變化及糖水偏愛程度;通過強(qiáng)迫游泳實(shí)驗(yàn)、懸尾實(shí)驗(yàn)測定大鼠不動(dòng)時(shí)間以及曠場實(shí)驗(yàn)測定大鼠中央格停留時(shí)間、水平穿越格數(shù)、站立次數(shù)、修飾次數(shù)的變化;并測定末次給藥24 h后大鼠血清中腫瘤壞死因子α(TNF-α)、皮質(zhì)醇、白細(xì)胞介素6(IL-6)、5-羥色胺(5-HT)水平。結(jié)果:與生理鹽水組比較,模型組大鼠的體質(zhì)量增加值、攝食量、糖水偏愛百分比均降低;強(qiáng)迫游泳和懸尾實(shí)驗(yàn)的不動(dòng)時(shí)間延長;曠場實(shí)驗(yàn)的中央格停留時(shí)間延長、水平穿越格數(shù)、站立次數(shù)、修飾次數(shù)均減少;血清中TNF-α、皮質(zhì)醇水平升高,IL-6、5-HT水平降低,差異均有統(tǒng)計(jì)學(xué)意義(P0.05或P0.01)。與模型組比較,除綠萼梅總黃酮低劑量組大鼠糖水偏愛百分比、強(qiáng)迫游泳不動(dòng)時(shí)間、曠場實(shí)驗(yàn)修飾次數(shù)以及綠萼梅總黃酮低、中劑量組大鼠血清中IL-6、5-HT水平改善不明顯外,其余各給藥組大鼠上述指標(biāo)均明顯改善(P0.05或P0.01)。結(jié)論:綠萼梅總黃酮能明顯改善CUMS誘導(dǎo)的大鼠抑郁行為,其機(jī)制可能與抑制炎癥反應(yīng)、調(diào)節(jié)下丘腦-垂體-腎上腺軸功能有關(guān)。
[Abstract]:Aim: to investigate the effect and mechanism of total flavonoids of sepals on depressive behavior in rats with chronic stress depression. Methods: sixty rats were randomly divided into normal saline group, model group, fluoxetine group (positive control group, 20 mg/kg) and low, medium and high dose group (80160240 mg/kg), with 10 rats in each group. The depression model was induced by chronic unpredictable mild stress (CUMS) alone in all rats except the normal saline group. The rats were given corresponding drugs once a day for 28 days at the same time. The body mass, food intake and the degree of preference of sugar water were observed, the immobility time of rats was determined by forced swimming test, the time of central lattice residence, the number of horizontal traversals and the times of standing were measured by open field experiment. The levels of tumor necrosis factor- 偽 (TNF- 偽), cortisol, interleukin-6 (IL-6) and serotonin (5-HT) in the serum of rats were measured 24 hours after the last administration. Results: compared with the saline group, the increase of body mass, the intake and the percentage of sugar water preference of the model group were all decreased, the immobility time of forced swimming and tail suspension experiment was prolonged, and the central lattice residence time of open field experiment was prolonged. The levels of TNF- 偽 and cortisol increased and the level of IL-6 5-HT decreased significantly (P0.05 or P0.01). Compared with the model group, the percentage of sugar water preference, the immobility time of forced swimming, the times of modification in open field experiment and the total flavone of green sepals were lower in the low dose group than those in the middle dose group, but the level of IL-6 + 5-HT in the serum of the middle dose group was not significantly improved. The above indexes were significantly improved in the other groups (P0.05 or P0.01). Conclusion: the total flavonoids can obviously improve the depression induced by CUMS in rats. The mechanism may be related to the inhibition of inflammation and the regulation of hypothalamus-pituitary-adrenal axis function.
【作者單位】： 泉州醫(yī)學(xué)高等�？茖W(xué)校藥理學(xué)教研室;
【基金】：福建省中青年教師教育科研項(xiàng)目(No.JAT160848) 泉州醫(yī)學(xué)高等�？茖W(xué)校校級課題(No.XJ1424A)
【分類號】：R285.5;R-332

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