發(fā)布時(shí)間：2018-07-01 09:53

  本文選題：川芎嗪 + 急性脊髓損傷��； 參考：《中國現(xiàn)代醫(yī)學(xué)雜志》2017年07期

【摘要】：目的研究川芎嗪對SD大鼠急性脊髓損傷模型的保護(hù)作用及其對損傷段脊髓膠質(zhì)纖維酸蛋白(GFAP)及胞漿型磷脂酶A2(cPLA2)表達(dá)的影響。方法 SD大鼠87只,隨機(jī)分成假手術(shù)組(n=9)、生理鹽水組(n=39)、川芎嗪組(n=39)。其中,假手術(shù)組行椎板切除術(shù),生理鹽水組及川芎嗪組采用改良Allen打擊法復(fù)制大鼠急性脊髓損傷模型,以BBB(Basso,Beattie,Bresnahan)評分評價(jià)各組大鼠脊髓功能;術(shù)后川芎嗪組按200 mg/kg注射川芎嗪,假手術(shù)組、生理鹽水組注射等量生理鹽水,并于術(shù)后1、3、7、14和21 d處死部分大鼠獲取脊髓損傷標(biāo)本,采用免疫組織化學(xué)比較GFAP、cPLA2表達(dá)情況,采用蘇木精-伊紅染色法(HE)染色觀察組織受損情況,并對GFAP、cPLA2進(jìn)行相關(guān)性分析。結(jié)果術(shù)后第7天起,川芎嗪組BBB評分均高于生理鹽水組,差異具有統(tǒng)計(jì)學(xué)意義(P0.05);術(shù)后各時(shí)間段川芎嗪組GFAP及cPLA2表達(dá)陽性面積均低于生理鹽水組,差異具有統(tǒng)計(jì)學(xué)意義,且GFAP與cPLA2表達(dá)陽性面積呈正相關(guān)(r=0.826,P=0.043),而從HE染色可知,脊髓損傷21 d,生理鹽水組已經(jīng)出現(xiàn)膠質(zhì)瘢痕,川芎嗪組僅為缺血性改變。結(jié)論川芎嗪可通過下調(diào)GFAP、cPLA2表達(dá),并對急性脊髓損傷起到一定保護(hù)作用。
[Abstract]:Objective to study the protective effect of ligustrazine on the expression of glial fibrillary protein (GFAP) and cytoplasmic phospholipase A2 (cPLA2) in the spinal cord of SD rats. Methods Eighty-seven SD rats were randomly divided into three groups: sham operation group (n = 9), saline group (n = 39) and ligustrazine group (n = 39). Among them, the sham operation group underwent laminectomy, the saline group and the ligustrazine group were used to make the acute spinal cord injury model by modified Allen attack method, the spinal cord function was evaluated by the Allen score, and the ligustrazine group was injected with ligustrazine for 200 mg/kg after operation. The rats in sham operation group and saline group were injected with the same amount of normal saline, and then killed on the 1st, 3rd, 7th, 14th and 21st day after operation. The expression of GFAP cPLA2 was compared by immunohistochemistry. The tissue damage was observed by hematoxylin-eosin staining (HE), and the correlation between GFAP and cPLA2 was analyzed. Results the BBB scores in ligustrazine group were significantly higher than those in saline group (P0.05), and the positive areas of GFAP and cPLA2 in ligustrazine group were significantly lower than those in saline group. There was a positive correlation between GFAP and CPLA2 positive area (r = 0.826P ~ (0.043). From HE staining, the glial scar was found in the saline group at 21 days after spinal cord injury, while the ischemic change was found in the ligustrazine group. Conclusion Ligustrazine can down-regulate the expression of GFAP CPLA2 and play a protective role in acute spinal cord injury.
【作者單位】： 河南中醫(yī)藥大學(xué)第一附屬醫(yī)院;
【分類號(hào)】：R-332;R651.2
，

本文編號(hào)：2087392