本文選題：銅綠假單胞菌 + 羧芐青霉素��； 參考：《西北大學(xué)學(xué)報(自然科學(xué)版)》2016年06期

【摘要】：為了探究銅綠假單胞菌抗羧芐青霉素的機(jī)制,通過基因敲除以及克拉維酸鉀和羧芐青霉素協(xié)同實驗對羧芐青霉素抗性菌株進(jìn)行研究。在對羧芐青霉素具有抗性的轉(zhuǎn)座突變體基礎(chǔ)上敲除amp C后,其對羧芐青霉素抗性沒有變化;8株羧芐青霉素抗性轉(zhuǎn)座突變體菌株克拉維酸鉀協(xié)同實驗呈陽性。基因組中預(yù)測為β內(nèi)酰胺酶的PA5514基因的敲除突變菌株及過表達(dá)菌株對氨芐青霉素以及羧芐青霉素的敏感性也沒有變化。結(jié)果說明amp C及PA5514表達(dá)升高并不是羧芐青霉素抗性轉(zhuǎn)座突變體抗性產(chǎn)生的原因,基因組中存在新的受克拉維酸鉀抑制的β內(nèi)酰胺酶可能是銅綠假單胞菌抗羧芐青霉素的一種新機(jī)制。同時,克拉維酸鉀與羧芐青霉素聯(lián)合使用能夠提高羧芐青霉素對銅綠假單胞菌抗性菌株的治療效果。
[Abstract]:In order to investigate the mechanism of resistance of Pseudomonas aeruginosa to Carbenicillin, the resistant strains were studied by gene knockout and synergistic experiments of potassium clavulanate and carbenicillin. After knockout amp C on the basis of transposable mutants resistant to carbenicillin, there was no change in resistance to carbenicillin in 8 strains of carbenicillin resistant mutants, potassium clavulanate synergistic test was positive. The sensitivity of the knockout mutant and overexpression strain of PA5514 gene predicted to 尾 lactamase to ampicillin and carbenicillin did not change. The results showed that the increased expression of amp C and PA5514 was not the cause of resistance to Carbenicillin resistance translocation mutants. The existence of new 尾 lactamases inhibited by potassium clavulanate in the genome may be a new mechanism of resistance of Pseudomonas aeruginosa to carbenicillin. At the same time, the combination of potassium clavulanate and carbenicillin could improve the therapeutic effect of carbenicillin against Pseudomonas aeruginosa.
【作者單位】： 西北大學(xué)生命科學(xué)學(xué)院/西部資源及現(xiàn)代生物技術(shù)教育部重點(diǎn)實驗室;
【分類號】：R364.5
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本文編號：2071544