本文選題：低密度脂蛋白 + 高密度脂蛋白　； 參考：《天津醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的：高脂血癥對健康有很大的負(fù)面影響,受其長期作用血管可發(fā)生動脈粥樣硬化(atheoselorsis, As),而動脈粥樣硬化是導(dǎo)致心腦血管疾病的重要原因,其發(fā)生與食物、生活習(xí)慣、遺傳等多種因素有關(guān),研究證明人體血液中低密度脂蛋白(low density lipoprotein, LDL)的升高以及由此而引起LDL進(jìn)入血管壁中并被氧化成氧化態(tài)的低密度脂蛋白(Ox-LDL)是導(dǎo)致As的主要原因。另一方面,高密度脂蛋白(high density lipoprotein, HDL)能阻止As的形成及加重。如果能有效控制血中LDL水平而又不引起HDL的損失,不僅能防止As的發(fā)生,而且對已形成的As有明顯減輕作用,進(jìn)而有利于心腦血管疾病的防治。目前,用于降低血液中低密度脂蛋白(low density lipoprotein, LDL)的吸附分離材料尚存在需要改進(jìn)的地方。設(shè)計(jì)合成出預(yù)計(jì)對低密度脂蛋白有較好吸附效果的HB-H-8樹脂,通過體外實(shí)驗(yàn)探討HB-H-8樹脂吸附低密度脂蛋白的有效性和特異性。 方法：HB-H-8樹脂與其他吸附材料的比較實(shí)驗(yàn)：將合成的HB-H-8樹脂與其他常用吸附材料進(jìn)行對比實(shí)驗(yàn),這些吸附材料包括陰離子交換樹脂,陽離子交換樹脂,弱極性樹脂,日本炭化樹脂,海藻酸鈉固定化多粘菌素B和大孔吸附樹脂。通過對20例LDL高于正常范圍的患者血清標(biāo)本進(jìn)行體外靜態(tài)吸附實(shí)驗(yàn),測定7種吸附材料吸附前后LDL濃度,計(jì)算其吸附率,比較實(shí)驗(yàn)結(jié)果。HB-H-8樹脂對LDL吸附性能的研究：HB-H-8樹脂經(jīng)過處理之后使用其對LDL高于正常范圍的患者血清標(biāo)本進(jìn)行體外動態(tài)考察HB-H-8的吸附飽和時(shí)間以及溫度對HB-H-8吸附效果的影響；使用HB-H-8進(jìn)行靜態(tài)吸附實(shí)驗(yàn),測定血清中低密度脂蛋白濃度(1owdensity lipoprotein, LDL)高密度脂蛋白(high density lipoprotein, HDL)總膽固醇(cholesterol total, CHO),甘油三酯(triglyceride, TG)吸附前后濃度變化,計(jì)算吸附率考察HB-H-8的吸附特異性。 結(jié)果：7種吸附材料吸附LDL比較實(shí)驗(yàn)結(jié)果表明HB-H-8樹脂對LDL平均吸附率為63.2%,其吸附能力高于其他6種吸附樹脂。HB-H-8體外動態(tài)吸附結(jié)果表明,HB-H-8樹脂對低密度脂蛋白(LDL)的吸附飽和時(shí)間為2個(gè)小時(shí),最適吸附溫度為恒溫37℃,體外靜態(tài)吸附結(jié)果顯示HB-H-8樹脂對LDL平均吸附率為63.2%,而對高密度脂蛋白(high density lipoprotein, HDL)的平均吸附率為1.9%。 結(jié)論：HB-H-8樹脂對LDL具有良好的特異性吸附性能,有望開發(fā)為一種低密度脂蛋白的血液凈化醫(yī)用吸附材料。
[Abstract]:Objective: hyperlipidemia has a great negative effect on health. Atherosclerosis is an important cause of cardiovascular and cerebrovascular diseases, and it is associated with food and living habits. Genetic analysis and other factors have shown that the increase of low density lipoprotein (low density lipoprotein,) in human blood and the oxidized low density lipoprotein (Ox-LDL) into the vascular wall are the main causes of as. High density lipoprotein (HDL), on the other hand, prevents the formation and aggravation of as. If the level of LDL in blood can be effectively controlled without causing HDL loss, it can not only prevent the occurrence of as, but also alleviate the formation of as, which is beneficial to the prevention and treatment of cardiovascular and cerebrovascular diseases. At present, there is still room for improvement in adsorptive separation materials used to reduce low density lipoprotein (low density lipoprotein,) in blood. HB-H-8 resin, which is expected to adsorb low density lipoprotein, was designed and synthesized. The effectiveness and specificity of HB-H-8 resin in adsorption of low density lipoprotein were investigated by experiments in vitro. Methods compared with other adsorption materials, the synthesized HB-H-8 resin was compared with other commonly used adsorption materials, including anion exchange resin, cation exchange resin, weakly polar resin. Japanese carbonized resin, sodium alginate immobilized polymyxin B and macroporous resin. In vitro static adsorption experiments were carried out on 20 serum samples of patients with LDL above normal range. The concentration of LDL was measured before and after the adsorption of 7 kinds of adsorption materials, and the adsorption rate was calculated. Study on the adsorption performance of HB-H-8 resin on LDL after treatment, the effect of temperature on the adsorption of HB-H-8 on HB-H-8 was investigated dynamically in vitro by using HB-H-8 resin in serum samples of patients whose LDL was higher than normal range. HB-H-8 was used to carry out static adsorption experiment. The concentrations of (cholesterol total, Cho and triglyceride (TG) in serum of 1owdensity lipoprotein (LDL-C), high density lipoprotein (high density lipoprotein,) and triglyceride (TG) were determined before and after adsorption. The adsorption specificity of HB-H-8 was investigated by calculating the adsorption rate of HB-H-8. Results the average adsorption rate of LDL on HB-H-8 resin was 63.2%, and the adsorption ability of HB-H-8 resin was higher than that of other six kinds of adsorption resin. HB-H-8 resin showed that HB-H-8 resin could adsorb low density lipoprotein (LDL) in vitro. The adsorption saturation time is 2 hours. The optimum adsorption temperature was 37 鈩，

本文編號：2069311