本文選題：烏頭反藥組合 + 甲醛致痛��； 參考：《中國(guó)中醫(yī)基礎(chǔ)醫(yī)學(xué)雜志》2017年04期

【摘要】：目的:考察“半蔞貝及攻烏”反藥組合對(duì)制川烏鎮(zhèn)痛效果的影響并探索相關(guān)機(jī)制。方法:基于甲醛足底皮下注射致痛模型,檢測(cè)致痛小鼠給藥后2個(gè)時(shí)相的舔足時(shí)間,明確制川烏和法半夏、栝樓、川貝母、浙貝母、白及各單藥及1∶1、1∶2和1∶3配伍組合的鎮(zhèn)痛效果,并通過聯(lián)用κ阿片受體激動(dòng)劑U-69593或拮抗劑Nor-BNI以及ELISA檢測(cè)致炎足NO、PGE2和TNF-α含量方法,進(jìn)一步分析相關(guān)作用機(jī)制。結(jié)果:制川烏單用能明顯縮短致痛小鼠2個(gè)時(shí)相的舔足時(shí)間,配伍法半夏、栝樓、浙貝母和白及后此作用減弱,呈量效關(guān)系。制川烏聯(lián)用Nor-BNI后可使舔足時(shí)間延長(zhǎng)至不給藥的狀態(tài);聯(lián)用U-69593后舔足時(shí)間比之前縮短,再配伍法半夏、白及后舔足時(shí)間明顯延長(zhǎng);制川烏單用能明顯降低甲醛致痛小鼠炎癥足中NO、PGE2和TNF-α的含量,配伍法半夏、栝樓、浙貝母和白及后相關(guān)因子含量升高。結(jié)論:降低烏頭的鎮(zhèn)痛功效可能是“半蔞貝及攻烏”反藥組合相反的具體表現(xiàn)之一,其機(jī)制與干擾κ阿片受體活化及增高炎癥局部促炎介質(zhì)含量有關(guān)。
[Abstract]:Aim: to investigate the analgesic effect of the antidrug combination of Phellodendron chinensis and Gongwu on the analgesic effect of Radix Aconitum mongolicum and to explore the related mechanism. Methods: based on the model of pain induced by subcutaneous injection of formaldehyde into plantar, the licking time of two phases after administration of the drug was detected in mice, and the time of licking foot was determined to determine the preparation of Radix Polygoni, Trichosanthes kirilowii, Fritillaria thunbergii and Thunb. The analgesic effects of each single drug and 1: 1: 1: 1: 2 and 1:3 combination were studied, and the related mechanisms were further analyzed by the combination of U-69593 or Nor-BNI, as well as Elisa to detect the levels of NOPGE2 and TNF- 偽 in inflamed feet. Results: the time of lapping feet in two phases of pain induced mice could be shortened obviously by single use of Radix Polygoni. The effect of compatibility of Pinellia ternata, Trichosanthes kirilowii, Fritillaria thunbergii and White was weakened, showing a dose-effect relationship. After using Nor-BNI, the time of licking foot was prolonged to the state of no administration, the time of licking foot was shorter than that before, and the time of licking foot was obviously prolonged after the combination of U-69593 and the method of compatibility with Pinellia ternata, white and after licking. The contents of NONP-GE2 and TNF- 偽 in the inflammatory feet of mice with formaldehyde-induced pain were significantly decreased, and the contents of related factors such as Pinellia ternata, Trichosanthes kirilowii, Fritillaria thunbergii and white were increased. Conclusion: reducing the analgesic effect of aconitum may be one of the opposite manifestations of anti-drug combination of "half Trichosanthes" and "Gongwu", and its mechanism is related to interfering with the activation of 魏 opioid receptor and increasing the content of inflammatory mediators.
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本文編號(hào)：2063459