本文選題：腸缺血再灌注 + 腸黏膜屏障　； 參考：《延邊大學(xué)》2011年碩士論文

【摘要】：[目的]復(fù)制大鼠腸缺血再灌注損傷模型,探討TLR2在大鼠腸缺血再灌注損傷中的改變及其意義。旨在求證腸缺血再灌注損傷后小腸的廣泛炎性損傷及后續(xù)的SIRS或MODS與小腸TLR2介導(dǎo)的天然免疫間關(guān)系。[方法]采用腸系膜上動脈夾閉法建立小腸缺血再灌注模型,將60只健康Wistar大鼠隨機分為正常對照組A(6只)、假手術(shù)組B(6只)和小腸缺血再灌注(IIR)損傷模型組(48只),其中根據(jù)腸系膜上動脈的不同夾閉時間(30min、40min、50min)分為C-E組,并計算大鼠的24小時內(nèi)死亡率并找出模型的最佳夾閉時間段。之后用此夾閉時間造出模型,并分為(2h、6h、12h、24h、48h)F-J等5組在各組的相應(yīng)時間取材,用酶聯(lián)免疫吸附法(ELISA)檢測各組織中的DAO和TLR2含量,并且取部分小腸做HE染色做比較。[結(jié)果]IIR后可見HE染色的腸組織中有明顯改變,并.且大鼠血清、小腸組織、peyer淋巴結(jié)及肝組織的TLR2和血清中DAO的含量與正常對照組相比均明顯增加(p0.05)。TLR2與DAO的改變有相關(guān)性；peyer淋巴結(jié)中有TLR2的表達(dá),并且與正常對照組相比有升高(p0.05),但與其他組織中TLR2反應(yīng)較慢。[結(jié)論]腸缺血再灌注損傷可導(dǎo)致多種組織TLR2表達(dá)增強,其改變與腸源性感染發(fā)生、發(fā)展和腸粘膜屏障的破壞有關(guān),其機制可能與小腸黏膜TLR2炎癥介質(zhì)信號傳導(dǎo)通路全面激活,所引起的天然免疫反應(yīng)及獲得性免疫反應(yīng)有關(guān)。
[Abstract]:[objective] to study the changes and significance of TLR2 in intestinal ischemia reperfusion injury in rats. The aim of this study was to identify the extensive inflammatory injury of small intestine following intestinal ischemia-reperfusion injury and the relationship between Sirs or mods and TLR2 mediated innate immunity in the small intestine. [methods] the intestinal ischemia-reperfusion model was established by clamping the superior mesenteric artery. Sixty healthy Wistar rats were randomly divided into normal control group (n = 6), sham-operated group B (n = 6) and intestinal ischemia-reperfusion (IIR) model group (n = 48). According to the different clamping time of superior mesenteric artery (30 min, 40 min or 50 min), the rats were divided into C-E group. The 24-hour mortality of rats was calculated and the optimal clamping time of the model was found. Then the model was made with this clamping time, and was divided into 5 groups (2 h ~ 6 h ~ 12 h ~ 24 h ~ 48 h) F-J and so on. The contents of Dao and TLR2 in tissues were detected by enzyme linked immunosorbent assay (Elisa), and some small intestine were stained with HE for comparison. [results] after IIR, there were obvious changes in the intestinal tissue stained by HE. The levels of TLR2 and Dao in serum, small intestinal lymph nodes and liver tissues in rats were significantly higher than those in normal controls (p0.05) .TLR2 and Dao were correlated with the expression of TLR2 in the lymph nodes of rats. Compared with the normal control group, TLR2 was increased (p0.05), but the TLR2 reaction was slower than that in other tissues. [conclusion] intestinal ischemia-reperfusion injury can result in increased expression of TLR2 in various tissues. The changes of TLR2 expression may be related to the occurrence and development of enterogenic infection and the destruction of intestinal mucosal barrier. The mechanism may be related to the activation of TLR2 inflammatory signal transduction pathway in intestinal mucosa. The innate immune response and the acquired immune response are related.
【學(xué)位授予單位】：延邊大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R363

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