本文選題：癲癇點燃模型 + 動情周期��； 參考：《寧夏醫(yī)科大學》2011年碩士論文

【摘要】：目的研究杏仁核電刺激點燃的癲癇模型大鼠動情周期、卵巢形態(tài)學及生長卵泡的超微變化。 方法取35只8周齡SD雌性大鼠,隨機分為癲癇模型組(n=15)、假手術(shù)組(n=10)和對照組(n=10)。癲癇模型組,雙極電極植入大鼠左側(cè)杏仁基底外側(cè)核,給予連續(xù)電刺激,建立電點燃的癲癇模型;假手術(shù)組,電極植入后不給予電刺激;對照組為正常雌性大鼠。所有大鼠每天清晨行陰道細胞涂片,以監(jiān)測動情周期。癲癇模型大鼠在點燃20d后于動情周期的間期取雙側(cè)卵巢組織,同期其他兩組大鼠也分別于動情間期摘取雙側(cè)卵巢組織,右側(cè)卵巢做病理切片,HE染色后鏡下閱片,采用Image-Pro Plus 6.0分析軟件進行形態(tài)學分析,左側(cè)卵巢選擇竇狀卵泡進行透射電鏡觀察。 結(jié)果癲癇模型組大鼠失去正常動情周期,日均體重增加的克數(shù)及卵巢重量均較假手術(shù)組及對照組顯著增高(P0.05);病理顯示,癲癇模型組大鼠卵巢切面面積增大,竇前卵泡、總生長卵泡數(shù)及閉鎖卵泡顯著增多(P0.05),卵巢發(fā)生多囊性改變;電鏡下顯示癲癇組大鼠竇狀卵泡中的顆粒細胞凋亡增多,增生的卵泡膜細胞更加富含脂滴。 結(jié)論癲癇發(fā)作可影響大鼠排卵功能,并導致大鼠發(fā)生卵巢多囊性改變,生長卵泡凋亡增多,卵泡膜細胞增生分泌增強。
[Abstract]:Objective to study the estrous cycle, ovarian morphology and ultrastructural changes of growth follicles in epileptic rats induced by amygdala kindled by nuclear stimulation.
Methods 35 SD female rats of 8 week age were randomly divided into epileptic model group (n=15), sham operation group (n=10) and control group (n=10), epileptic model group, bipolar electrode implanted in the left amygdala lateral nucleus of rat left amygdala, to give continuous electrical stimulation, to establish epileptic model of electric kindling; the sham operation group was not given electrical stimulation after the electrode implantation; the control group was normal female. Rats. All rats were smeared with vaginal cells every morning to monitor the estrous cycle. The rats in the epileptic model rats were taken bilateral ovarian tissue at the interval of the estrous cycle after the 20d was ignited. The other two groups of rats were also taken bilateral ovarian tissues at the estrus interval, and the right ovary was sectioning for pathological sections. After HE staining, the rats were examined under the microscope, and Image-Pro Plus was used. 6 the morphological analysis of the software was performed, and the antral follicles in the left ovaries were observed by transmission electron microscopy.
Results the normal estrous cycle was lost in the epileptic model group, the average daily weight gain and the ovarian weight were significantly higher than those in the sham operation group and the control group (P0.05). The pathology showed that the area of the ovary cut area of the epileptic model group increased, the preantral follicle, the total number of follicles and the closed follicles increased significantly (P0.05), and the ovarian polycystic changes were changed. Electron microscopy showed that the apoptosis of granulosa cells increased significantly in the epileptic rats, and that the proliferated follicle cells were more rich in lipid droplets.
Conclusion epileptic seizures can affect ovulation function in rats, and cause ovarian polycystic changes. Growth follicle apoptosis increases and follicular cell proliferation and secretion increase.
【學位授予單位】：寧夏醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R742.1;R-332

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相關(guān)期刊論文 前1條

1 陳子江,李媛,周燦權(quán),黃荷鳳,劉嘉茵,喬杰,黃國寧,梁曉燕,郁琦;多囊卵巢綜合征與輔助生殖的熱點問題討論[J];現(xiàn)代婦產(chǎn)科進展;2004年04期

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