本文選題：兔 + 下頜骨�。� 參考：《佳木斯大學(xué)》2012年碩士論文

【摘要】：目的：探討兔下頜骨來(lái)源的骨髓間充質(zhì)干細(xì)胞（bone marrow mesenchymal stemcells, BMMSCs）體外分離、培養(yǎng)及其生物學(xué)行為。 方法：取兔下頜骨中的松質(zhì)骨，密度梯度離心分離法收集單個(gè)核細(xì)胞，，貼壁培養(yǎng)，取第P2或P3代檢測(cè)：（1）倒置顯微鏡下觀察細(xì)胞生長(zhǎng)狀態(tài)；（2）MTT法測(cè)定細(xì)胞增殖，測(cè)定的數(shù)據(jù)應(yīng)用SPSS16.0進(jìn)行統(tǒng)計(jì)學(xué)分析，繪制細(xì)胞生長(zhǎng)增殖曲線；（3）克隆形成實(shí)驗(yàn)計(jì)算克隆形成率；（4）向成骨細(xì)胞、脂肪細(xì)胞、骨骼肌細(xì)胞誘導(dǎo)分化；（5）流式細(xì)胞儀檢測(cè)細(xì)胞表面抗原標(biāo)志。 結(jié)果：（1）細(xì)胞經(jīng)傳代后形態(tài)一致，呈梭形或三角形；（2）細(xì)胞生長(zhǎng)曲線顯示下頜骨BMMSCs經(jīng)歷潛伏期、對(duì)數(shù)生長(zhǎng)期和平臺(tái)期，統(tǒng)計(jì)數(shù)據(jù)表明細(xì)胞1—7d生長(zhǎng)增殖有統(tǒng)計(jì)學(xué)意義（P＜0.05）；（3）集落形成實(shí)驗(yàn)克隆形成率37%；（4）成骨誘導(dǎo)后形成鈣結(jié)節(jié)，茜素紅染色呈陽(yáng)性；成脂誘導(dǎo)細(xì)胞核周圍出現(xiàn)脂滴，油紅O染色呈陽(yáng)性；成骨骼肌細(xì)胞誘導(dǎo)desmin抗原標(biāo)記陽(yáng)性；（5）流式細(xì)胞儀檢測(cè)結(jié)果顯示所獲得的下頜骨BMMSCs純度較高，CD90和CD146表面標(biāo)記陽(yáng)性率分別為98.7%、98.1%。 結(jié)論：兔下頜骨分離出的BMMSCs，純度較高，有強(qiáng)大的自我復(fù)制及增殖能力，具有多向誘導(dǎo)分化的潛能。
[Abstract]:Aim: to investigate the isolation, culture and biological behavior of bone marrow mesenchymal stem cells (marrow mesenchymal stemcells, BMMSCs) derived from rabbit mandible in vitro. Methods: the cancellous bone was collected from rabbit mandible and mononuclear cells were collected and cultured by density gradient centrifugation. The data were analyzed statistically by SPSS 16.0. The clone forming rate was calculated by the clone forming experiment. The colony formation rate was calculated. The clone formation rate was 4) differentiation into osteoblasts, adipocytes and skeletal muscle cells. Cell surface antigen markers were detected by flow cytometry. Results the cell growth curve showed that the mandibular BMMSCs experienced latent period, logarithmic growth period and plateau phase. The statistical data showed that the proliferation of BMMSCs in 1-7 days was statistically significant (P < 0.05). The colony forming rate of colony formation was 37%. After osteogenesis, calcium nodules were formed, alizarin red staining was positive, lipid droplets appeared around the nucleus of adipogenic cells, oil red O staining was positive, skeletal muscle cells induced desmin antigen labeling. The results of flow cytometry showed that the positive rates of CD90 and CD146 on the surface of BMMSCs were 98.7and 98.1, respectively. Conclusion: BMMSCs isolated from rabbit mandible have high purity, strong ability of self-replication and proliferation, and have the potential to induce differentiation.
【學(xué)位授予單位】：佳木斯大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R329

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