本文選題：靜磁場 + 成骨細胞　； 參考：《南昌大學》2011年碩士論文

【摘要】：目的：探討靜磁場(SMF)作用下金屬離子對成骨細胞生物活性的影響,為防治假體周圍無菌性松動提供一定的理論依據(jù)。 方法：將CoCl2粉末與CrCl3粉末溶于無菌雙蒸水配制成CoCl2和CrCl3金屬離子混合溶液,將金屬離子Co2+、Cr3+混合溶液與小鼠顱骨成骨細胞(MC3T3-E1)共培養(yǎng),根據(jù)實驗分組加載不同磁場強度(1、10、100mT)。實驗分為Co2++Cr3+組(對照組)；Co2+、Cr3++1mT; Co2+、Cr3++10mT; Co2+、Cr3++100mT。掃描電鏡檢測成骨細胞表面超微結(jié)構(gòu)。MTT法檢測成骨細胞活力。流式細胞術(shù)檢測細胞周期分布。酶聯(lián)免疫分析(ELISA)法對培養(yǎng)上清液ALP(堿性磷酸酶)含量進行檢測。 結(jié)果：靜磁場作用下的成骨細胞呈現(xiàn)更加成熟的形態(tài)學特征,Co2+、Cr3+金屬離子對成骨細胞MC3T3的毒性明顯降低；同時G2M(分裂期)分布比例明顯增加,細胞停滯在GoG1(休眠期)的比率明顯減少；與對照組相比,不同場強磁場加載后,成骨細胞堿性磷酸酶分泌明顯增強(P0.05)。 結(jié)論：靜磁場作用下Co2+、Cr3+金屬離子對成骨細胞的毒性作用會明顯減低,為磁場療法防治假體周圍骨溶解提供了實驗依據(jù)。
[Abstract]:Aim: to investigate the effect of metal ions on the biological activity of osteoblasts under the action of SMF in order to provide a theoretical basis for the prevention and treatment of aseptic loosening around prosthesis. Methods: CoCl2 powder and CrCl3 powder were dissolved in aseptic double distilled water to form a mixed solution of CoCl2 and CrCl3 metal ions. The mixed solution of CoCl2 and CrCl3 was co-cultured with MC3T3-E1 of mouse skull osteoblasts. The experiment was divided into two groups: Co2Cr3 group (control group), Co2Cr310mTand Co2Cr3100mT. The surface ultrastructure of osteoblasts was examined by scanning electron microscope. MTT assay was used to detect the viability of osteoblasts. Cell cycle distribution was detected by flow cytometry. Enzyme linked immunosorbent assay (Elisa) was used to detect ALP (alkaline phosphatase) in culture supernatant. Results: under the action of static magnetic field, osteoblasts showed more mature morphological characteristics. The toxicity of Co _ 2 / Cr _ 3 to MC3T3 was significantly decreased, and the distribution of G _ 2M (mitotic phase) was significantly increased. Compared with the control group, the alkaline phosphatase secretion of osteoblasts increased significantly after different magnetic fields. Conclusion: the toxicity of Co _ 2 / Cr _ (3) metal ions to osteoblasts can be significantly reduced under the action of static magnetic field, which provides experimental basis for magnetic field therapy to prevent and treat osteolysis around prosthesis.
【學位授予單位】：南昌大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R329

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相關(guān)期刊論文 前2條

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本文編號：2033949