本文選題：高血壓 + 高脂血癥�。� 參考：《福建醫(yī)科大學(xué)》2011年碩士論文

【摘要】：目的(1)尋找一種存活率高且能快速制作出高血壓腦動脈硬化模型的方法。(2)探尋家兔腦動脈硬化的早期觀察指標(biāo)。 方法42只新西蘭大白兔隨機(jī)分為7組:A組:對照組,B組:雙腎動脈0.70mm狹窄組,C組:右腎切除左腎動脈0.70mm狹窄組,D組:雙腎動脈0.55mm狹窄組,E組:右腎切除左腎動脈0.55mm狹窄組,F組:雙腎動脈0.45mm狹窄組,G組:右腎切除左腎動脈0.45mm狹窄組。分別于術(shù)前(0周)、術(shù)后1、2、4、8、12周測血壓,術(shù)后開始高脂喂養(yǎng),高脂喂養(yǎng)前(0周)、高脂飼料飼喂4、8、12周測血脂,12周后等壓灌注固定,取頸總動脈、胸主動脈、主動脈弓、腦組織做病理檢測,并測量腦動脈血管外徑(D)、內(nèi)徑(d),計(jì)算D/d-1的值。 結(jié)果C組、D組各有2只家兔死亡,E組3只家兔死亡,F組、G組全部死亡,其余家兔均完成實(shí)驗(yàn)。D組、E組在第1周血壓顯著升高(P0.01),并持續(xù)至實(shí)驗(yàn)結(jié)束。0周時各組間血脂水平無顯著差別(P0.05),4周時至12周時實(shí)驗(yàn)組TC、LDL較對照組顯著升高(P0.05)。實(shí)驗(yàn)組頸總動脈、胸主動脈及主動脈弓均可見頸動脈粥樣硬化斑塊。腦動脈D/d-1的值:VG染色及actin染色A組與B組無顯著差異(P0.05),其余各組間比較P=0.000。 結(jié)論1.以高血壓并高脂血癥制作的家兔模型有顱內(nèi)動脈硬化的表現(xiàn)。2.D/d-1的值可作為家兔腦動脈硬化的早期觀察指標(biāo)。3.高血壓腦動脈硬化模型以雙側(cè)腎動脈0.55mm狹窄聯(lián)合高脂喂養(yǎng)法制作存活率高、血壓升高時間早、操作簡便。
[Abstract]:Objective 1) to find a method of high survival rate and rapid establishment of hypertensive cerebral arteriosclerosis model. Methods 42 New Zealand white rabbits were randomly divided into 7 groups: control group B: bilateral renal artery 0.70mm stenosis group C group: right nephrectomy left renal artery 0.70mm stenosis group D group: double renal artery 0.55mm stenosis group E group: right nephrectomy left renal artery 0.55mm stenosis group F group: double renal artery 0.45mm stenosis group G group: right nephrectomy left renal artery 0.45mm stenosis group. The blood pressure was measured at 0 weeks before operation, 1 day and 2 minutes after operation. Blood pressure was measured at 12 weeks after operation. After operation, high fat feeding was started, and high fat diet was fed at 0 weeks before feeding. Blood lipids were measured and fixed with isobaric perfusion and fixation after 12 weeks. Common carotid artery, thoracic aorta and aortic arch were taken from the common carotid artery, thoracic aorta and aortic arch. The pathological examination of brain tissue and the measurement of the external diameter of cerebral artery and the internal diameter of the cerebral artery were performed to calculate the value of D / d-1. Results there were 2 rabbits in group C and 2 rabbits in group D, 3 rabbits in group E and 3 rabbits in group F, and all of them died in group G. All the other rabbits completed the experiment. The blood pressure in group E increased significantly at the first week, and there was no significant difference in blood lipid levels between the groups until the end of the experiment. The level of serum lipid in the experimental group was significantly higher than that in the control group from the 4th week to the 12th week after the end of the experiment, and the level of TCfU LDL in the experimental group was significantly higher than that in the control group. Carotid atherosclerotic plaques were found in the common carotid artery, thoracic aorta and aortic arch in the experimental group. There was no significant difference between group A and group B (P 0.05), and there was no significant difference between group A and group B (P 0.05), and there was no significant difference between group A and group B (P 0.05), and the other groups were compared with each other (P = 0.000). Conclusion 1. The model made with hypertension and hyperlipidemia has the manifestation of intracranial arteriosclerosis. 2. The value of D / d-1 can be used as an early observation index of cerebral arteriosclerosis in rabbits. Hypertensive cerebral arteriosclerosis model was made with bilateral renal artery 0.55mm stenosis combined with high fat feeding method with high survival rate, early blood pressure rise time and simple operation.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R743.3;R-332

【參考文獻(xiàn)】

相關(guān)期刊論文 前7條

1 張成英,陳前芬;兔大腦中動脈的解剖學(xué)觀察[J];蚌埠醫(yī)學(xué)院學(xué)報(bào);1993年03期

2 張磊,陳國榮,鄭榮遠(yuǎn),王群姬,謝淑萍,王擁軍;高脂飼料加空氣干燥術(shù)建立兔頸動脈粥樣硬化模型[J];中國動脈硬化雜志;2001年02期

3 肖敏,王瑋,黨書毅,朱艷霞,張群林,黃從新;血管內(nèi)皮生長因子165對實(shí)驗(yàn)性兔動脈粥樣硬化斑塊形成的影響[J];中國動脈硬化雜志;2005年04期

4 李秋梅,王碩仁,趙明鏡,崔巍,呂希瀅,戴欣,高樹彪;家兔新型動脈粥樣硬化狹窄模型的建立及其動態(tài)觀察[J];中國實(shí)驗(yàn)動物學(xué)報(bào);2004年01期

5 劉國榮,李月春,張京芬;缺血性腦血管病顱內(nèi)動脈狹窄的危險因素[J];中華神經(jīng)科雜志;2003年05期

6 王興海,傅群武,劉暢,唐雷,洪輝文,原林,黃文華,鐘世鎮(zhèn);“虛擬中國人”建模的動脈灌注研究[J];中國臨床解剖學(xué)雜志;2002年05期

7 張勇,婁桂予,錢民章;建立兔高脂血癥模型及動脈粥樣硬化斑塊的方法[J];遵義醫(yī)學(xué)院學(xué)報(bào);2001年04期

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