本文選題：全反式維甲酸 + 凋亡��； 參考：《重慶醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的：探討全反式維甲酸對阿霉素腎病模型小鼠腎臟保護(hù)作用及其可能的作用機(jī)制。 方法：6-8周齡的雄性BALB/C小鼠隨機(jī)分為正常組、阿霉素腎病組（模型組）和全反式維甲酸治療組（治療組）。一次性尾靜脈注射阿霉素10.5mg/kg體重，建立阿霉素腎病小鼠模型。自阿霉素腎病模型建立成功后2W開始，ATRA治療組給予20mg/kg/次的ATRA腹腔注射（3次/周），ATRA溶解在玉米油中；正常組、模型組腹腔注射給予等次、等量玉米油（3次/周），干預(yù)至10W。各組分別于注射阿霉素后第6、10周末處死6只小鼠，通過免疫組織化學(xué)方法檢測小鼠腎臟Fas、FasL的表達(dá)水平；Real-time PCR法檢測小鼠腎組織Fas和FasL以及全反式維甲酸受體RARa、RXRa的mRNA的表達(dá)水平。 結(jié)果：與模型組相比，正常組、治療組尿蛋白水平、血脂膽固醇（TC）、甘油三酯（TG）水平明顯降低，差異有統(tǒng)計學(xué)意義(P0.05)，血清白蛋白（ALB）的水平明顯升高，差異有統(tǒng)計學(xué)意義（P0.05）。免疫組化結(jié)果顯示Fas、FasL的表達(dá)量，與模型組相比，正常組和治療組陽性表達(dá)明顯減少，差異有統(tǒng)計學(xué)意義（P0.05）；熒光定量PCR顯示，，與模型組相比，正常組和治療組的Fas、FasL mRNA的表達(dá)量明顯減低，差異顯著有統(tǒng)計學(xué)意義（P0.01）。第10周全反式維甲酸受體RARa、RXRa的mRNA的表達(dá)水平，治療組與正常組相和模型組相比表達(dá)明顯增加，差異有統(tǒng)計學(xué)意義（P＜0.05）。 結(jié)論：ATRA可以明顯減輕阿霉素腎病模型小鼠的腎臟損傷，降低的蛋白尿水平，降低血清TG、TC的水平，這可能是與其拮抗腎臟固有細(xì)胞的凋亡蛋白Fas、FasL有關(guān)。
[Abstract]:Aim: to investigate the protective effect of all trans retinoic acid on kidney of adriamycin nephropathy model mice and its possible mechanism. Methods male BALB / R / C mice aged 6-8 weeks were randomly divided into normal group, adriamycin nephropathy group (model group) and all-trans retinoic acid treatment group (treatment group). The model of adriamycin nephropathy in mice was established by single tail vein injection of adriamycin 10.5mg/kg. Two weeks after the adriamycin nephropathy model was established, the 20mg/kg/ treatment group was given intraperitoneal injection of 20mg/kg/ three times per week to dissolve in corn oil, while in the normal group, the model group was given the same intraperitoneal injection of the same amount of corn oil three times a week, and the intervention lasted for 10 wt. Six mice were killed at the end of the 6th week after injection of adriamycin. The expression of FasL in kidney was detected by immunohistochemical method and the mRNA expression of FAS, FasL and RARARARARXRa were detected by Real-time PCR. Results: compared with the model group, the levels of urinary protein, serum lipids, cholesterol, triglyceride and TGwere significantly decreased in the normal group and treatment group, and the difference was statistically significant (P 0.05), and the level of serum albumin (ALB) was significantly increased (P 0.05). The results of immunohistochemistry showed that the positive expression of Fas-FasL in normal group and treatment group decreased significantly compared with the model group, and the difference was statistically significant (P0.05A), the fluorescence quantitative PCR showed that compared with the model group, the positive expression of Fas-FasL was significantly lower than that of the model group. The expression of Fas-FasL mRNA in normal group and treatment group was significantly lower than that in control group (P 0.01). At the 10th week, the expression of RXRa mRNA in all trans retinoic acid receptors was significantly increased in the treatment group compared with the normal group and the model group (P < 0.05). Conclusion 1: ATRA can significantly reduce renal injury, decrease the level of proteinuria and decrease the level of serum TGTc in the model mice with adriamycin nephropathy, which may be related to the antagonism of Fas-FasL, the apoptotic protein of renal inherent cells.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R-332;R692.9

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 施靜;炎癥加重脂質(zhì)誘導(dǎo)內(nèi)質(zhì)網(wǎng)應(yīng)激致人腎小球系膜細(xì)胞損害的分子機(jī)制[D];重慶醫(yī)科大學(xué);2013年

本文編號：2033663