本文選題：稀土納米材料 + 成骨細(xì)胞　； 參考：《河北大學(xué)》2012年碩士論文

【摘要】：本文以納米CeO_2、La_2O_3和Y_2O_3三種稀土納米材料為研究對象，在對其理化性質(zhì)充分表征的基礎(chǔ)上，以原代分離的小鼠成骨細(xì)胞為模型，采用四甲基偶氮唑藍(lán)法、堿性磷酸酶活性檢測、油紅O染色、茜素紅染色等測定方法在細(xì)胞水平研究了納米CeO_2、La_2O_3和Y_2O_3對成骨細(xì)胞增殖、分化及礦化功能的影響；并采用流式細(xì)胞儀對CeO_2納米顆粒的攝取情況進(jìn)行了研究。 結(jié)果表明： 1.實驗所用的納米材料分別為：粒徑分別約為30nm和50nm的近似為球形的CeO_2納米顆粒，形貌分別為不規(guī)則片狀和短棒狀的納米La_2O_3，粒徑約為30nm的近似為球形納米Y_2O_3，這幾種納米材料在培養(yǎng)基中均發(fā)生了不同程度的團聚現(xiàn)象。 2.納米CeO_2對小鼠原代成骨細(xì)胞增殖、分化及礦化功能的影響依賴于納米顆粒的尺寸、濃度和孵育時間等因素。50nm CeO_2對OBs的細(xì)胞增殖有一定抑制作用，但是30nm CeO_2在同樣的劑量下則沒有表現(xiàn)出抑制效果。兩種尺寸的CeO_2納米顆粒均能促進(jìn)OBs礦化結(jié)節(jié)的形成，并且這種促進(jìn)作用呈劑量依賴效應(yīng)，但是促進(jìn)效果隨著納米CeO_2的尺度減小而有增大的趨勢。 3.納米La_2O_3和Y_2O_3對小鼠原代成骨細(xì)胞增殖、礦化及成脂分化的影響依賴于納米顆粒的形貌、劑量、化學(xué)組成和孵育時間等因素。同等劑量下片狀納米La_2O_3對細(xì)胞增殖的抑制效果明顯高于棒狀納米La_2O_3的抑制作用，，納米Y_2O_3的細(xì)胞毒性要低于同等劑量納米La_2O_3；兩種形貌的納米La_2O_3在濃度0.6μg/mL以上時均能促進(jìn)OBs礦化結(jié)節(jié)的形成，且促進(jìn)作用呈劑量依賴效應(yīng)，但是片狀納米La_2O_3對OBs礦化作用的促進(jìn)率明顯高于棒狀納米La_2O_3。 4. CeO_2納米顆粒與成骨細(xì)胞作用24h后會進(jìn)入到細(xì)胞內(nèi)部，并且隨濃度的增大進(jìn)入細(xì)胞的量增多。
[Abstract]:In this paper, three kinds of rare earth nanomaterials, namely, CeO2L _ 2O _ 3 and Ys _ 2O _ 3, were used as research objects. On the basis of fully characterizing their physical and chemical properties, the primary isolated mouse osteoblasts were used as model, the activity of alkaline phosphatase was determined by tetramethyl azolyl blue method. Oil red O staining and alizarin red staining were used to study the effects of CeO2LAS and Y _ 2O _ 3 on the proliferation, differentiation and mineralization of osteoblasts at the cell level, and the uptake of CEO _ 2 nanoparticles by flow cytometry. The results show that: 1. The nanomaterials used in the experiment are: CeO-2 nanoparticles with approximately 30nm and 50nm particle sizes, which are approximately spherical, The morphology of La _ 2O _ 3 nanocrystalline is irregular flake and short rod shape, and the particle size of 30nm is approximately spherical Y _ 2O _ 3. These nanomaterials are agglomerated to different degrees in the medium. 2. The effects of CeO2 nanoparticles on the proliferation, differentiation and mineralization of primary mouse osteoblasts depended on the size, concentration and incubation time of nanoparticles. 50 nm CeO2 could inhibit the proliferation of osteoblasts. But 30nm CEO _ 2 showed no inhibitory effect at the same dose. Both CeO-2 nanoparticles could promote the formation of mineralized nodules of OBs, and the effect was dose-dependent, but the effect increased with the decrease of CeO2 size. 3. The effects of the nano La _ 2O _ 3 and Y _ S _ 2O _ 3 on the proliferation, mineralization and adipogenic differentiation of primary osteoblasts depend on the morphology, dosage, chemical composition and incubation time of the nanoparticles. At the same dose, the inhibitory effect of tippal-like nano-La2O3 on cell proliferation was significantly higher than that of rod-shaped nano-La2O3, and the cytotoxicity of nano-sized La2O3 was lower than that of the same dose of nano-La2O3; Both kinds of nano-La _ 2O _ 3 could promote the formation of mineralized nodules of OBs at concentrations above 0.6 渭 g / mL, and the effect was dose-dependent. However, the promotion rate of nano-La _ 2O _ 3 was significantly higher than that of rod-shaped nano-La2O3.4. After contact with osteoblasts for 24 hours, CeO-2 nanoparticles will enter into the cells and the amount of them will increase with the increase of concentration.
【學(xué)位授予單位】：河北大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R329

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 周國強;谷廣其;王文穎;王書香;張金超;;納米材料在生物醫(yī)學(xué)中的應(yīng)用[J];河北大學(xué)學(xué)報(自然科學(xué)版);2012年02期

2 周國強;王文穎;劉慧玲;王書香;張金超;;CeO_2納米顆粒對過氧化氫所致A549細(xì)胞損傷的保護作用[J];環(huán)境與健康雜志;2012年02期

3 ;The effects of gold nanoparticles on the proliferation, differentiation, and mineralization function of MC3T3-E1 cells in vitro[J];Chinese Science Bulletin;2010年11期

4 常雪靈;祖艷;趙宇亮;;納米毒理學(xué)與安全性中的納米尺寸與納米結(jié)構(gòu)效應(yīng)[J];科學(xué)通報;2011年02期

5 洪廣言;稀土產(chǎn)業(yè)與納米科技[J];稀土信息;2002年05期

6 洪廣言;;稀土納米材料的應(yīng)用研究[J];稀土信息;2007年11期

本文編號：2032443