本文選題：自體血回輸 + 紅細(xì)胞��； 參考：《暨南大學(xué)》2012年碩士論文

【摘要】：目的利用原子力顯微鏡(AFM)觀察自體血回輸對(duì)紅細(xì)胞形貌及膜超微結(jié)構(gòu)的影響。 方法選取擇期行脊柱手術(shù)的患者10例，年齡35~65歲，ASAI~III級(jí)，所有患者均采用靜脈全麻，術(shù)中按常規(guī)方法應(yīng)用自體血液回收機(jī)。切皮前采集外周靜脈血2ml（T1），采集蓄血罐中回收血即洗滌前血液2ml（T2），采集清洗完畢后，，輸血袋內(nèi)紅細(xì)胞懸液即洗滌后血液2ml（T3），以上血液樣本均用肝素抗凝。所有樣本采用熒光正置顯微鏡放大400倍下觀察紅細(xì)胞，并計(jì)算其畸形率。利用AFM觀察紅細(xì)胞膜表面超微結(jié)構(gòu)的變化以及紅細(xì)胞膜表面粗糙度。 結(jié)果 （1）T1時(shí)刻紅細(xì)胞畸形率（3.12±1.75）%低于T2（9.82±2.30）%和T3（9.62±2.67）%（P0.05）,而后兩者之間并無(wú)統(tǒng)計(jì)學(xué)差異（P0.05）。 （2）回收血和靜脈血紅細(xì)胞AFM形貌、紅細(xì)胞膜表面蛋白顆粒分布均存在差異。T2紅細(xì)胞膜表面平均粗糙度Ra（16.40±5.04）、均方根粗糙度Rq（20.24±5.89)高于T1（Ra：4.05±0.53；Rq：5.07±0.65）和T3（Ra：4.51±0.81；Rq：5.73±1.69）時(shí)刻收集的紅細(xì)胞（P0.05），而后兩者紅細(xì)胞膜表面粗糙度并無(wú)統(tǒng)計(jì)學(xué)差異（P0.05）。 結(jié)論自體血液回收可增加紅細(xì)胞畸形率及改變紅細(xì)胞AFM形貌及膜表面超微結(jié)構(gòu)。
[Abstract]:Objective to observe the effect of autologous blood transfusion on erythrocyte morphology and membrane ultrastructure by atomic force microscope (AFM). Methods Ten patients (35 ~ 65 years old with ASAI III grade) undergoing spinal surgery were selected. All patients were treated with intravenous general anesthesia and autologous blood recovery machine was used in the operation. The peripheral venous blood 2ml / T _ 1 was collected before skin incision, and the blood was recovered from the blood storage tank before washing. After the collection and cleaning, the blood was washed with 2ml / T _ (3) of the red blood cell suspension in the blood transfusion bag, and the above blood samples were all treated with heparin anticoagulant. All samples were magnified 400 times by fluorescence positive microscope to observe red blood cells and calculate their deformity rate. The ultrastructure of erythrocyte membrane surface and the surface roughness of erythrocyte membrane were observed by AFM. Results at T1, the erythrocyte malformation rate was 3.12 鹵1.75%, which was lower than that of T2P 9.82 鹵2.30% and T3 9.62 鹵2.67% P0.05, but there was no significant difference between the two groups. The mean surface roughness of erythrocyte membrane was 16.40 鹵5.04, RqN 20.24 鹵5.89), which was higher than that of T1 Raw 4.05 鹵0.53 RQ: 5.07 鹵0.65) and T3 Raw 4.51 鹵0.81; RQ: 5.73 鹵1.69) the erythrocyte membrane surface roughness of RQ: 5.73 鹵1.69 was not significantly different from that of RQ: 5.73 鹵1.69. Conclusion the recovery of autologous blood can increase the rate of erythrocyte malformation and change the morphology of erythrocyte AFM and the ultrastructure of membrane surface.
【學(xué)位授予單位】：暨南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R329

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相關(guān)期刊論文 前9條

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本文編號(hào)：2029107