本文選題：手足口病 + 腸道病毒型　； 參考：《南京醫(yī)科大學(xué)學(xué)報(bào)(自然科學(xué)版)》2017年07期

【摘要】：目的:探討2014—2015年江蘇省腸道病毒71型(enterovirus 71,EV71)分離株的病毒基因特征。方法:用RD細(xì)胞對(duì)RT-PCR初篩EV71陽性的標(biāo)本進(jìn)行病毒分離,成功分離到的EV71分離株進(jìn)行VP1區(qū)的序列測(cè)定和分析。序列分析結(jié)果用DNA Star和MEGA 6.0軟件進(jìn)行分析,并與各型參考株進(jìn)行比較。結(jié)果:共分離到35株EV71型毒株,分離陽性率為29.17%。35株EV71分離株VP1區(qū)的核苷酸和氨基酸序列同源性分別為93.3%~100.0%和96.6%~100.0%,與EV71的C4a亞型代表株安徽阜陽株的核苷酸和氨基酸序列同源性分別為95.6%~97.9%和97.9%~100.0%;在系統(tǒng)進(jìn)化樹上與C4a亞型代表株相聚在一起,并且存在2個(gè)進(jìn)化分支。結(jié)論:2014—2015年江蘇省分離的35株EV71毒株均屬于C4a亞型;江蘇省EV71流行株可能與國內(nèi)其他省市流行的病毒有著共同的祖先,再各自進(jìn)化。
[Abstract]:Objective: to study the gene characteristics of enterovirus 71 (EV71) isolated from Jiangsu Province in 2014-2015. Methods: the EV71 positive specimens screened by RT-PCR were isolated by Rd cells, and the EV71 isolates were sequenced and analyzed. The sequence analysis results were analyzed by DNA Star and Mega 6.0 software, and compared with the reference strains. Results: 35 strains of EV71 were isolated. The nucleotide and amino acid sequence homology of the VP1 region of the isolated strain 29.17.35 was 93.3% and 96.6100.0%, respectively. The nucleotide and amino acid sequence homology with the C4a subtype of EV71 in Fuyang strain of Anhui Province was 95.697. 9% and 97. 997. 0% respectively, and the nucleotide and amino acid sequence homology was 97. 990. 0. 00.The nucleotide and amino acid sequence homology of EV71 C4a subtype strain was 95. 6% and 97. 9% respectively. The chemotypic tree was joined with the representative strain of C4a subtype. And there are two evolutionary branches. Conclusion 35 EV71 strains isolated from Jiangsu Province in 2014-2015 belong to C4a subtype, and the EV71 epidemic strains in Jiangsu Province may share a common ancestor with other provinces and cities in China, and then evolve separately.
【作者單位】： 江蘇省疾病預(yù)防控制中心;蘇州市疾病預(yù)防控制中心;
【基金】：國家自然科學(xué)基金青年基金(81402732) 江蘇省預(yù)防醫(yī)學(xué)課題(Y2012071) 蘇州市科技局應(yīng)用基礎(chǔ)研究課題(SYS201262)
【分類號(hào)】：R373.2

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