本文選題：旋毛蟲(chóng) + Hela細(xì)胞��； 參考：《吉林大學(xué)》2011年碩士論文

【摘要】：為了深入研究旋毛蟲(chóng)抗腫瘤的的作用,本試驗(yàn)先在體外利用MTT和細(xì)胞凋亡的檢測(cè)的方法初步確定了旋毛蟲(chóng)可溶性抗原可以使Hela細(xì)胞發(fā)生凋亡,在用ELISA方法初步確定了旋毛蟲(chóng)與Hela細(xì)胞間存在共同抗原,并利用SDS-PAGE和Western-blotting方法測(cè)得了相關(guān)抗原的分子量。運(yùn)用離心超濾裝置及透析等技術(shù)對(duì)相關(guān)抗原進(jìn)行了分離,FITC-免疫組化法對(duì)該抗原進(jìn)行了定位分析。在此基礎(chǔ)上,建立了Hela細(xì)胞裸鼠移植瘤動(dòng)物模型,并對(duì)旋毛蟲(chóng)與Hela細(xì)胞相關(guān)抗原抗體進(jìn)行了抗腫瘤實(shí)驗(yàn)。 實(shí)驗(yàn)結(jié)果顯示:通過(guò)MTT法和細(xì)胞凋亡實(shí)驗(yàn)證明,旋毛蟲(chóng)可溶性抗原可以對(duì)Hela細(xì)胞的增殖具有抑制作用,ELISA法初步證明旋毛蟲(chóng)與Hela細(xì)胞間存在較強(qiáng)的免疫交叉反應(yīng);利用SDS-PAGE和Western-blotting方法獲得了旋毛蟲(chóng)抗原與Hela細(xì)胞相關(guān)抗原的分子量分別是40KDa和55KDa。免疫組化定位分析該相關(guān)抗原位于Hela細(xì)胞表面抗原和旋毛蟲(chóng)的消化道上皮細(xì)胞膜上;應(yīng)用相關(guān)抗原抗體在裸鼠體內(nèi)進(jìn)行抗腫瘤試驗(yàn)表明,相關(guān)抗原抗體具有抗腫瘤作用,抑瘤率為82.3%,且效果略好于可溶性抗原抗體組。首次以旋毛蟲(chóng)與Hela細(xì)胞相關(guān)抗原抗體為切入點(diǎn)研究旋毛蟲(chóng)抗腫瘤的機(jī)理,為旋毛蟲(chóng)抗腫瘤機(jī)制的研究與開(kāi)發(fā)提供了理論依據(jù)。
[Abstract]:In order to study the antitumor effect of Trichinella spiralis in vitro, MTT and apoptosis were used to determine that soluble antigen of Trichinella spiralis could induce apoptosis in Hela cells. The common antigen between Trichinella spiralis and Hela cells was preliminarily determined by Elisa, and the molecular weight of the related antigen was measured by SDS-PAGE and Western-blotting. The antigens were isolated by centrifugation ultrafiltration and dialysis. The antigen was localized by FITC- immunohistochemical method. On this basis, an animal model of Hela cell xenografts in nude mice was established, and the antitumor effects of Trichinella spiralis and Hela cell related antigens were tested. The results showed that the soluble antigen of Trichinella spiralis could inhibit the proliferation of Hela cells by MTT assay and apoptosis assay. Elisa showed that there was a strong immune cross reaction between Trichinella spiralis and Hela cells. The molecular weights of Trichinella spiralis antigen and Hela cell associated antigen were 40 KDa and 55 KDa respectively by SDS-PAGE and Western-blotting. Immunohistochemical analysis showed that the associated antigen was located on the surface antigen of Hela cell and the membrane of digestive tract epithelium of Trichinella spiralis. The inhibition rate was 82.3%, and the effect was slightly better than that of soluble antigen antibody group. The antitumor mechanism of Trichinella spiralis was studied by using the antibody against Hela cells for the first time, which provided a theoretical basis for the research and development of antitumor mechanism of Trichinella spiralis.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R392;R73-35

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 任保彥;旋毛蟲(chóng)與MCF-7乳腺癌細(xì)胞相關(guān)蛋白抗體抗腫瘤效應(yīng)研究[D];吉林大學(xué);2012年

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本文編號(hào)：2026922