本文選題：金簇 + 去鐵蛋白��； 參考：《吉林大學(xué)》2011年碩士論文

【摘要】：有良好生物兼容性,低毒性,和穩(wěn)定性的可以靶向組織或細(xì)胞納米結(jié)構(gòu)在納米生物醫(yī)學(xué)上已經(jīng)引起了很大的興趣。本文成功用“點(diǎn)控制”的技術(shù)在去鐵蛋白內(nèi)部可控合成兩個(gè)協(xié)同作用的可以經(jīng)激發(fā)產(chǎn)生熒光的金簇。并通過(guò)測(cè)定熒光光譜,證實(shí)隨著金簇尺寸的增大和之間距離的減少,兩金簇之間的能量共振轉(zhuǎn)移(Forster resonance energy transfer, FRET)會(huì)使兩個(gè)金簇總的熒光強(qiáng)度逐漸增強(qiáng),并使熒光光譜發(fā)生紅移。通過(guò)冷凍電鏡(Cryo-electron microscopy)和透射電鏡(transmission electron microscopy)的低分辨,高分辨模式和掃描模式分別得到成對(duì)金簇在蛋白內(nèi)部的直觀圖像。在掃描模式下還得到金元素的X射線(xiàn)能量散射圖譜(energy-dispersive-X-ray spectroscopy, EDX)證明確實(shí)是在去鐵蛋白中組裝成功雙金簇。通過(guò)測(cè)定Au-Ft (apoferritin-far-red Au cluster)的X-射線(xiàn)近邊吸收峰,證明是在去鐵蛋白的兩個(gè)重鏈上組裝上的雙金簇。 成功的在有去鐵蛋白受體的Caco-2細(xì)胞及小鼠的腎臟部位得到了特定的生物醫(yī)學(xué)靶向熒光成像。這些實(shí)驗(yàn)結(jié)果表明,利用生物體本身存在的具有靶向功能的蛋白質(zhì)和無(wú)機(jī)納米材料組裝體,有可能成為一種解決包括腫瘤在內(nèi)的各種難以攻克疾病的診斷及治療的潛在方法。相關(guān)文章已經(jīng)被JACS (IF=8.58)接收。
[Abstract]:Good biocompatibility, low toxicity, and stability of targeted tissue or cell nanostructures have attracted considerable interest in nanomedicine. In this paper, the "point control" technique has been successfully used to control the synthesis of two clusters of gold which can excite and produce fluorescence within the deferritin. The fluorescence spectra show that with the increase of the gold cluster size and the decrease of the distance between the two gold clusters, the energy resonance transfer resonance energy transfer, fret between the two gold clusters will gradually increase the total fluorescence intensity of the two gold clusters, and make the fluorescence spectrum red shift. The low resolution, high resolution and scanning modes of cryopreservation electron microscopy (Cryo-electron microscopy) and transmission electron microscopy (electron) were used to obtain the visual images of gold clusters inside the protein, respectively. The X-ray energy scattering spectra of gold elements were also obtained in scanning mode. It was proved that the double gold clusters were successfully assembled in ferritin. By measuring the X- ray absorption peak of Au-Ft apoferritin-far-red gold cluster, it is proved that it is a double gold cluster assembled on two heavy chains of ferritin. Specific biomedical targeted fluorescence imaging was successfully obtained in Caco-2 cells with ferritin receptor and in the kidney of mice. These results indicate that the use of proteins and inorganic nanomaterials with targeted function, which exists in organisms themselves, may become a potential method for the diagnosis and treatment of various difficult diseases, including tumors. Related articles have been received by JACS IFF 8.58).
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類(lèi)號(hào)】：R341

【共引文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前2條

1 張莉;智能靶向納米凝膠的構(gòu)建及其抗腫瘤機(jī)制研究[D];第二軍醫(yī)大學(xué);2013年

2 王靜;尺寸均一的多功能納米粒在惡性黑素瘤成像和治療中的應(yīng)用[D];華中科技大學(xué);2013年

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本文編號(hào)：2014068