本文選題：細(xì)胞芯片 + 細(xì)胞組裝��； 參考：《杭州電子科技大學(xué)》2012年碩士論文

【摘要】：生命科學(xué)、信息科學(xué)與制造科學(xué)相結(jié)合,是二十一世紀(jì)科技發(fā)展的重要趨勢。基于先進(jìn)制造和組織工程理論的細(xì)胞三維受控組裝技術(shù),以及基于微加工技術(shù)和傳感器技術(shù)的細(xì)胞芯片技術(shù),為組織工程、藥物篩選、環(huán)境監(jiān)測等研究領(lǐng)域拓展了新的理論和技術(shù)空間。本文將細(xì)胞三維受控組裝技術(shù)引入細(xì)胞芯片的制作中,探討細(xì)胞三維受控組裝與細(xì)胞芯片技術(shù)結(jié)合的技術(shù)方案。 首先,論文對細(xì)胞可控裝配技術(shù)和細(xì)胞芯片的國內(nèi)外研究現(xiàn)狀進(jìn)行了綜述,系統(tǒng)分析了細(xì)胞組裝技術(shù)和細(xì)胞芯片技術(shù)結(jié)合的理論和技術(shù)可能�；诩�(xì)胞組裝的技術(shù)特征和細(xì)胞傳感器的工作原理,選取了MEA傳感器和細(xì)胞電阻抗分析器,并對其參數(shù)、選材和制作過程進(jìn)行了研究,設(shè)計并制作了適合組裝細(xì)胞的集成型細(xì)胞芯片。對芯片性能的測試顯示,芯片能在低頻段顯示出良好的阻抗性,保證較好的導(dǎo)電性能,在高頻段顯示良好的容性,對測試細(xì)胞電生理活性具有良好的靈敏度。 然后,論文詳細(xì)分析了細(xì)胞三維受控組裝機的構(gòu)架,并對噴頭模塊進(jìn)行擴(kuò)展和改進(jìn)。面向芯片表面細(xì)胞裝配,選取了適合芯片電生理檢測的生物混合材料,并對材料的配比進(jìn)行了系統(tǒng)研究。在此基礎(chǔ)上,研究了基質(zhì)材料與細(xì)胞芯片的結(jié)合特性：成形和固化特性、與芯片基底結(jié)合的穩(wěn)定性、材料對細(xì)胞電化學(xué)檢測的影響。 其次,根據(jù)集成型細(xì)胞芯片的結(jié)構(gòu)特點研究了細(xì)胞在芯片表面的組裝路徑,確定了最佳方案。在此基礎(chǔ)上,研究了成形參數(shù)、噴頭大小和交聯(lián)方式對組裝結(jié)構(gòu)的整體性、基質(zhì)材料連續(xù)性及其粘結(jié)性和微絲直徑大小的影響。結(jié)果顯示,細(xì)胞可以按照設(shè)計的模式準(zhǔn)確組裝到指定傳感器區(qū)域,路徑連貫穩(wěn)定,與芯片的粘結(jié)性良好,微絲直徑符合組裝精度要求。 最后,比較了DMEM、PBS溶液相比氰鐵化鉀溶液作為芯片測試液的差異,結(jié)果顯示DMEM可在高頻段作為芯片測試的電解液。隨后我們在芯片上組裝了多種細(xì)胞,結(jié)果顯示用細(xì)胞三維受控組裝技術(shù)能精確地將不同細(xì)胞組裝到細(xì)胞芯片表面的傳感器區(qū)域,細(xì)胞芯片測得的細(xì)胞增殖情況與標(biāo)準(zhǔn)的生化檢測方法測得的情況一致,細(xì)胞芯片能準(zhǔn)確的測得并分辨不同細(xì)胞增殖情況,芯片在藥物篩選、病理研究等領(lǐng)域顯示出巨大的應(yīng)用價值。
[Abstract]:The combination of life science, information science and manufacturing science is an important trend in the development of science and technology in 21 century. Three-dimensional controlled cell assembly technology based on advanced manufacturing and tissue engineering theory, and cell chip technology based on microprocessing and sensor technology, for tissue engineering, drug screening, Environmental monitoring and other research fields have expanded the new theoretical and technical space. In this paper, the cell three-dimensional controlled assembly technology is introduced into the fabrication of cell chip, and the technical scheme of cell three-dimensional controlled assembly and cell chip technology is discussed. This paper summarizes the research status of cell controlled assembly technology and cell chip at home and abroad, and systematically analyzes the theory and technology possibility of cell assembly technology and cell chip technology combination. Based on the technical characteristics of cell assembly and the working principle of cell sensor, MEA sensor and cell impedance analyzer are selected, and their parameters, material selection and fabrication process are studied. An integrated cell chip suitable for assembly cells was designed and fabricated. The chip performance test shows that the chip can display good impedance in low frequency band, ensure good conductivity, good capacitance in high frequency band, good sensitivity to test cell electrophysiological activity. The architecture of cell three-dimensional controlled assembly machine is analyzed in detail, and the nozzle module is extended and improved. For cell assembly on chip surface, biological mixture materials suitable for chip electrophysiological detection were selected, and the proportion of materials was studied systematically. On the basis of this, the binding properties of matrix material and cell chip are studied: forming and solidifying characteristics, stability of binding to chip substrate, influence of material on cell electrochemical detection. According to the structural characteristics of the integrated cell chip, the cell assembly path on the chip surface was studied, and the optimal solution was determined. On this basis, the effects of forming parameters, nozzle size and crosslinking mode on the integrity of the assembly structure, the matrix material continuity, its adhesion and the diameter of the microfilament were studied. The results show that the cells can be accurately assembled to the designated sensor area according to the designed mode, the path is coherent and stable, the adhesion to the chip is good, and the diameter of the microfilament meets the requirements of the assembly accuracy. The difference between DMEMN PBS solution and potassium cyanide solution as chip test solution was compared. The results showed that DMEM could be used as electrolyte for chip test in high frequency range. We then assembled a variety of cells on the chip, and the results showed that the three dimensional controlled cell assembly technique could accurately assemble different cells into the sensor area on the surface of the cell chip. The cell proliferation measured by the cell chip is consistent with that measured by the standard biochemical method. The cell chip can accurately measure and distinguish the proliferation of different cells, and the microarray is in drug screening. Pathological research and other fields have shown great application value.
【學(xué)位授予單位】：杭州電子科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R329

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 張安兄,呂德龍,鐘偉,程為莊,杜強國;天然生物材料構(gòu)建組織工程支架的研究進(jìn)展[J];北京生物醫(yī)學(xué)工程;2005年05期

2 徐瑩;楊勇;鄒紹芳;王平;;基于微加工技術(shù)的集成細(xì)胞芯片的實驗研究[J];傳感技術(shù)學(xué)報;2008年06期

3 裴瑩;鄭學(xué)晶;湯克勇;;膠原及明膠基天然高分子復(fù)合材料研究進(jìn)展[J];高分子通報;2010年02期

4 劉根起,趙曉鵬;明膠-海藻酸鈉半互穿聚合物網(wǎng)絡(luò)膜及其電刺激響應(yīng)行為的研究[J];功能材料;2003年04期

5 崔俊鋒,尹玉姬,張福江,葉芬,姚康德;明膠基生物材料在組織工程中的應(yīng)用[J];國外醫(yī)學(xué).生物醫(yī)學(xué)工程分冊;2004年01期

6 劉海霞,顏永年,王小紅,熊卓,程捷;肝細(xì)胞的三維受控組裝[J];清華大學(xué)學(xué)報(自然科學(xué)版);2005年08期

7 劉豐;張人佶;顏永年;劉海霞;;明膠/海藻酸鈉組織工程支架的快速成形[J];清華大學(xué)學(xué)報(自然科學(xué)版);2006年08期

8 展義臻;朱平;董朝紅;郭肖青;;海藻酸共混纖維的發(fā)展現(xiàn)狀[J];染整技術(shù);2006年12期

9 韋曉蘭,莫志宏;細(xì)胞傳感器與芯片的研究進(jìn)展[J];生物化學(xué)與生物物理進(jìn)展;2004年09期

10 王秋君;徐銘恩;李艷蕾;袁梅娟;胡金夫;;一種基于混流控制的多細(xì)胞三維受控組裝系統(tǒng)[J];生物醫(yī)學(xué)工程學(xué)雜志;2011年05期

，

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